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Volumn 516, Issue , 2012, Pages 37-62

Cyclotide isolation and characterization

Author keywords

Cyclic peptides; Cyclotides; Kalata B1; Mass spectrometry; Membrane binding; Structure

Indexed keywords

COMPLEMENTARY DNA; CYCLOTIDE; PRIMER RNA; PROTEIN PRECURSOR; RNA;

EID: 84867021192     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-394291-3.00024-1     Document Type: Chapter
Times cited : (20)

References (63)
  • 2
    • 0038322581 scopus 로고    scopus 로고
    • Linearization of a naturally occurring circular protein maintains structure but eliminates hemolytic activity
    • DOI 10.1021/bi027323n
    • D.G. Barry, N.L. Daly, R.J. Clark, L. Sando, and D.J. Craik Linearization of a naturally occurring circular protein maintains structure but eliminates hemolytic activity Biochemistry 42 2003 6688 6695 (Pubitemid 36666109)
    • (2003) Biochemistry , vol.42 , Issue.22 , pp. 6688-6695
    • Barry, D.G.1    Daly, N.L.2    Clark, R.J.3    Sando, L.4    Craik, D.J.5
  • 3
    • 0026953522 scopus 로고
    • Identification and characterization of a full-length cDNA encoding for an auxin-induced 1-aminocyclopropane-1-carboxylate synthase from etiolated mung bean hypocotyl segments and expression of its mRNA in response to indole-3-acetic acid
    • J.R. Botella, J.M. Arteca, C.D. Schlagnhaufer, R.N. Arteca, and A.T. Phillips Identification and characterization of a full-length cDNA encoding for an auxin-induced 1-aminocyclopropane-1-carboxylate synthase from etiolated mung bean hypocotyl segments and expression of its mRNA in response to indole-3-acetic acid Plant Molecular Biology 20 1992 425 436
    • (1992) Plant Molecular Biology , vol.20 , pp. 425-436
    • Botella, J.R.1    Arteca, J.M.2    Schlagnhaufer, C.D.3    Arteca, R.N.4    Phillips, A.T.5
  • 4
    • 79952477224 scopus 로고    scopus 로고
    • Protocol: A simple phenol-based method for 96-well extraction of high quality RNA from Arabidopsis
    • M.S. Box, V. Coustham, C. Dean, and J.S. Mylne Protocol: A simple phenol-based method for 96-well extraction of high quality RNA from Arabidopsis Plant Methods 7 2011 7
    • (2011) Plant Methods , vol.7 , pp. 7
    • Box, M.S.1    Coustham, V.2    Dean, C.3    Mylne, J.S.4
  • 5
    • 71849113184 scopus 로고    scopus 로고
    • Cyclotide proteins and precursors from the genus Gloeospermum: Filling a blank spot in the cyclotide map of Violaceae
    • R. Burman, C.W. Gruber, K. Rizzardi, A. Herrmann, D.J. Craik, and M.P. Gupta Cyclotide proteins and precursors from the genus Gloeospermum: Filling a blank spot in the cyclotide map of Violaceae Phytochemistry 71 2009 13 20
    • (2009) Phytochemistry , vol.71 , pp. 13-20
    • Burman, R.1    Gruber, C.W.2    Rizzardi, K.3    Herrmann, A.4    Craik, D.J.5    Gupta, M.P.6
  • 7
  • 8
    • 2442715239 scopus 로고    scopus 로고
    • Thermal, chemical, and enzymatic stability of the cyclotide kalata B1: The importance of the cyclic cystine knot
    • DOI 10.1021/bi049711q
    • M.L. Colgrave, and D.J. Craik Thermal, chemical, and enzymatic stability of the cyclotide kalata B1: The importance of the cyclic cystine knot Biochemistry 43 2004 5965 5975 (Pubitemid 38669464)
    • (2004) Biochemistry , vol.43 , Issue.20 , pp. 5965-5975
    • Colgrave, M.L.1    Craik, D.J.2
  • 9
    • 54349090107 scopus 로고    scopus 로고
    • The anthelmintic activity of the cyclotides: Natural variants with enhanced activity
    • M.L. Colgrave, A.C. Kotze, D.C. Ireland, C.K. Wang, and D.J. Craik The anthelmintic activity of the cyclotides: Natural variants with enhanced activity Chembiochem 9 2008 1939 1945
    • (2008) Chembiochem , vol.9 , pp. 1939-1945
    • Colgrave, M.L.1    Kotze, A.C.2    Ireland, D.C.3    Wang, C.K.4    Craik, D.J.5
  • 10
    • 0034650303 scopus 로고    scopus 로고
    • A vesicle capture sensor chip for kinetic analysis of interactions with membrane-bound receptors
    • DOI 10.1006/abio.1999.4389
    • M.A. Cooper, A. Hansson, S. Lofas, and D.H. Williams A vesicle capture sensor chip for kinetic analysis of interactions with membrane-bound receptors Analytical Biochemistry 277 2000 196 205 (Pubitemid 30055867)
    • (2000) Analytical Biochemistry , vol.277 , Issue.2 , pp. 196-205
    • Cooper, M.A.1    Hansson, A.2    Lofas, S.3    Williams, D.H.4
  • 12
    • 0033579483 scopus 로고    scopus 로고
    • Plant cyclotides: A unique family of cyclic and knotted proteins that defines the cyclic cystine knot structural motif
    • DOI 10.1006/jmbi.1999.3383
    • D.J. Craik, N.L. Daly, T. Bond, and C. Waine Plant cyclotides: A unique family of cyclic and knotted proteins that defines the cyclic cystine knot structural motif Journal of Molecular Biology 294 1999 1327 1336 (Pubitemid 30008803)
    • (1999) Journal of Molecular Biology , vol.294 , Issue.5 , pp. 1327-1336
    • Craik, D.J.1    Daly, N.L.2    Bond, T.3    Waine, C.4
  • 14
    • 4444331457 scopus 로고    scopus 로고
    • The role of the cyclic peptide backbone in the anti-HIV activity of the cyclotide kalata B1
    • DOI 10.1016/j.febslet.2004.08.007, PII S001457930400986X
    • N.L. Daly, K.R. Gustafson, and D.J. Craik The role of the cyclic peptide backbone in the anti-HIV activity of the cyclotide kalata B1 FEBS Letters 574 2004 69 72 (Pubitemid 39200896)
    • (2004) FEBS Letters , vol.574 , Issue.1-3 , pp. 69-72
    • Daly, N.L.1    Gustafson, K.R.2    Craik, D.J.3
  • 18
    • 0038700801 scopus 로고    scopus 로고
    • Expression of Viola cyclotides by liquid chromatography-mass spectrometry and tandem mass spectrometry sequencing of intercysteine loops after introduction of charges and cleavage sites by aminoethylation
    • DOI 10.1016/S0003-2697(03)00114-3
    • U. Göransson, A.M. Broussalis, and P. Claeson Expression of Viola cyclotides by liquid chromatography-mass spectrometry and tandem mass spectrometry sequencing of intercysteine loops after introduction of charges and cleavage sites by aminoethylation Analytical Biochemistry 318 2003 107 117 (Pubitemid 36627489)
    • (2003) Analytical Biochemistry , vol.318 , Issue.1 , pp. 107-117
    • Goransson, U.1    Broussalis, A.M.2    Claeson, P.3
  • 19
    • 0346220263 scopus 로고    scopus 로고
    • Disulfide mapping of the cyclotide kalata B1. Chemical proof of the cyclic cystine knot motif
    • DOI 10.1074/jbc.M308771200
    • U. Göransson, and D.J. Craik Disulfide mapping of the cyclotide kalata B1. Chemical proof of the cystic cystine knot motif The Journal of Biological Chemistry 278 2003 48188 48196 (Pubitemid 37523272)
    • (2003) Journal of Biological Chemistry , vol.278 , Issue.48 , pp. 48188-48196
    • Goransson, U.1    Craik, D.J.2
  • 21
    • 84861322262 scopus 로고    scopus 로고
    • Cyclotides, a novel ultrastable polypeptide scaffold for drug discovery
    • A. Gould, Y. Ji, T.L. Aboye, and J.A. Camarero Cyclotides, a novel ultrastable polypeptide scaffold for drug discovery Current Pharmaceutical Design 17 2011 4294 4307
    • (2011) Current Pharmaceutical Design , vol.17 , pp. 4294-4307
    • Gould, A.1    Ji, Y.2    Aboye, T.L.3    Camarero, J.A.4
  • 22
    • 58149347521 scopus 로고    scopus 로고
    • Cyclic peptides from Oldenlandia affinis DC. Molecular and biological properties
    • L. Gran, K. Sletten, and L. Skjeldal Cyclic peptides from Oldenlandia affinis DC. Molecular and biological properties Chemistry & Biodiversity 5 2008 2014 2022
    • (2008) Chemistry & Biodiversity , vol.5 , pp. 2014-2022
    • Gran, L.1    Sletten, K.2    Skjeldal, L.3
  • 26
  • 27
    • 84860123359 scopus 로고    scopus 로고
    • The importance of the cell membrane on the mechanism of action of cyclotides
    • S.T. Henriques, and D.J. Craik The importance of the cell membrane on the mechanism of action of cyclotides ACS Chemical Biology 7 2012 626 636
    • (2012) ACS Chemical Biology , vol.7 , pp. 626-636
    • Henriques, S.T.1    Craik, D.J.2
  • 28
    • 79959914450 scopus 로고    scopus 로고
    • Decoding the membrane activity of the cyclotide kalata B1: The importance of phosphatidylethanolamine phospholipids and lipid organization on hemolytic and anti-HIV activities
    • S.T. Henriques, Y.H. Huang, K.J. Rosengren, H.G. Franquelim, F.A. Carvalho, and A. Johnson Decoding the membrane activity of the cyclotide kalata B1: The importance of phosphatidylethanolamine phospholipids and lipid organization on hemolytic and anti-HIV activities The Journal of Biological Chemistry 286 2011 24231 24241
    • (2011) The Journal of Biological Chemistry , vol.286 , pp. 24231-24241
    • Henriques, S.T.1    Huang, Y.H.2    Rosengren, K.J.3    Franquelim, H.G.4    Carvalho, F.A.5    Johnson, A.6
  • 29
  • 30
    • 39149141320 scopus 로고    scopus 로고
    • The alpine violet, Viola biflora, is a rich source of cyclotides with potent cytotoxicity
    • A. Herrmann, R. Burman, J. Mylne, G. Karlsson, J. Gullbo, and D. Craik The alpine violet, Viola biflora, is a rich source of cyclotides with potent cytotoxicity Phytochemistry 69 2008 939 952
    • (2008) Phytochemistry , vol.69 , pp. 939-952
    • Herrmann, A.1    Burman, R.2    Mylne, J.3    Karlsson, G.4    Gullbo, J.5    Craik, D.6
  • 32
    • 33751073415 scopus 로고    scopus 로고
    • A novel suite of cyclotides from Viola odorata: Sequence variation and the implications for structure, function and stability
    • DOI 10.1042/BJ20060627
    • D.C. Ireland, M.L. Colgrave, and D.J. Craik A novel suite of cyclotides from Viola odorata: Sequence variation and the implications for structure, function and stability The Biochemical Journal 400 2006 1 12 (Pubitemid 44763814)
    • (2006) Biochemical Journal , vol.400 , Issue.1 , pp. 1-12
    • Ireland, D.C.1    Colgrave, M.L.2    Craik, D.J.3
  • 33
    • 33645047649 scopus 로고    scopus 로고
    • Discovery and characterization of a linear cyclotide from Viola odorata: Implications for the processing of circular proteins
    • D.C. Ireland, M.L. Colgrave, P. Nguyencong, N.L. Daly, and D.J. Craik Discovery and characterization of a linear cyclotide from Viola odorata: implications for the processing of circular proteins Journal of Molecular Biology 357 2006 1522 1535
    • (2006) Journal of Molecular Biology , vol.357 , pp. 1522-1535
    • Ireland, D.C.1    Colgrave, M.L.2    Nguyencong, P.3    Daly, N.L.4    Craik, D.J.5
  • 35
    • 79954580823 scopus 로고    scopus 로고
    • Analysis and classification of circular proteins in CyBase
    • Q. Kaas, and D.J. Craik Analysis and classification of circular proteins in CyBase Biopolymers 94 2010 584 591
    • (2010) Biopolymers , vol.94 , pp. 584-591
    • Kaas, Q.1    Craik, D.J.2
  • 36
    • 11844280405 scopus 로고    scopus 로고
    • Studies on the membrane interactions of the cyclotides kalata B1 and kalata B6 on model membrane systems by surface plasmon resonance
    • DOI 10.1016/j.ab.2004.10.028, PII S0003269704008462
    • H. Kamimori, K. Hall, D.J. Craik, and M.I. Aguilar Studies on the membrane interactions of the cyclotides kalata B1 and kalata B6 on model membrane systems by surface plasmon resonance Analytical Biochemistry 337 2005 149 153 (Pubitemid 40092791)
    • (2005) Analytical Biochemistry , vol.337 , Issue.1 , pp. 149-153
    • Kamimori, H.1    Hall, K.2    Craik, D.J.3    Aguilar, M.-I.4
  • 37
    • 0023047980 scopus 로고
    • Vesicles of variable sizes produced by a rapid extrusion procedure
    • L.D. Mayer, M.J. Hope, and P.R. Cullis Vesicles of variable sizes produced by a rapid extrusion procedure Biochimica et Biophysica Acta 858 1986 161 168
    • (1986) Biochimica et Biophysica Acta , vol.858 , pp. 161-168
    • Mayer, L.D.1    Hope, M.J.2    Cullis, P.R.3
  • 38
    • 30544439962 scopus 로고    scopus 로고
    • In-solution digestion of proteins for mass spectrometry
    • K.F. Medzihradszky In-solution digestion of proteins for mass spectrometry Methods in Enzymology 405 2005 50 65
    • (2005) Methods in Enzymology , vol.405 , pp. 50-65
    • Medzihradszky, K.F.1
  • 39
    • 30144443840 scopus 로고    scopus 로고
    • Peptide sequence analysis
    • DOI 10.1016/S0076-6879(05)02007-0, PII S0076687905020070, 7, Biological Mass Spectrometry
    • K.F. Medzihradszky Peptide sequence analysis Methods in Enzymology 402 2005 209 244 (Pubitemid 43053129)
    • (2005) Methods in Enzymology , vol.402 , pp. 209-244
    • Medzihradszky, K.F.1
  • 42
    • 84862833381 scopus 로고    scopus 로고
    • Cyclotides are a component of the innate defense of Oldenlandia affinis
    • J.S. Mylne, C.K. Wang, N.L. van der Weerden, and D.J. Craik Cyclotides are a component of the innate defense of Oldenlandia affinis Biopolymers 94 2010 635 646
    • (2010) Biopolymers , vol.94 , pp. 635-646
    • Mylne, J.S.1    Wang, C.K.2    Van Der Weerden, N.L.3    Craik, D.J.4
  • 43
    • 79959864353 scopus 로고    scopus 로고
    • Discovery and characterization of novel cyclotides originated from chimeric precursors consisting of albumin-1 chain a and cyclotide domains in the Fabaceae family
    • G.K. Nguyen, S. Zhang, N.T. Nguyen, P.Q. Nguyen, M.S. Chiu, and A. Hardjojo Discovery and characterization of novel cyclotides originated from chimeric precursors consisting of albumin-1 chain a and cyclotide domains in the Fabaceae family The Journal of Biological Chemistry 286 2011 24275 24287
    • (2011) The Journal of Biological Chemistry , vol.286 , pp. 24275-24287
    • Nguyen, G.K.1    Zhang, S.2    Nguyen, N.T.3    Nguyen, P.Q.4    Chiu, M.S.5    Hardjojo, A.6
  • 44
    • 34547511527 scopus 로고    scopus 로고
    • The cyclotide fingerprint in Oldenlandia affinis: Elucidation of chemically modified, linear and novel macrocyclic peptides
    • DOI 10.1002/cbic.200700097
    • M.R.R. Plan, U. Göransson, R.J. Clark, N.L. Daly, M.L. Colgrave, and D.J. Craik The cyclotide fingerprint in Oldenlandia affinis: Elucidation of chemically modified, linear and novel macrocyclic peptides Chembiochem 8 2007 1001 1011 (Pubitemid 47183617)
    • (2007) ChemBioChem , vol.8 , Issue.9 , pp. 1001-1011
    • Plan, M.R.R.1    Goransson, U.2    Clark, R.J.3    Daly, N.L.4    Colgrave, M.L.5    Craik, D.J.6
  • 45
    • 47849105966 scopus 로고    scopus 로고
    • Backbone cyclised peptides from plants show molluscicidal activity against the rice pest Pomacea canaliculate (golden apple snail)
    • DOI 10.1021/jf800302f
    • M.R. Plan, I. Saska, A.G. Cagauan, and D.J. Craik Backbone cyclised peptides from plants show molluscicidal activity against the rice pest Pomacea canaliculata (golden apple snail) Journal of Agricultural and Food Chemistry 56 2008 5237 5241 (Pubitemid 352039129)
    • (2008) Journal of Agricultural and Food Chemistry , vol.56 , Issue.13 , pp. 5237-5241
    • Plan, M.R.R.1    Saska, I.2    Cagauan, A.G.3    Craik, D.J.4
  • 47
    • 77949469889 scopus 로고    scopus 로고
    • Identification of candidates for cyclotide biosynthesis and cyclisation by expressed sequence tag analysis of Oldenlandia affinis
    • Q. Qin, E.J. McCallum, Q. Kaas, J. Suda, I. Saska, and D.J. Craik Identification of candidates for cyclotide biosynthesis and cyclisation by expressed sequence tag analysis of Oldenlandia affinis BMC Genomics 11 2010 111
    • (2010) BMC Genomics , vol.11 , pp. 111
    • Qin, Q.1    McCallum, E.J.2    Kaas, Q.3    Suda, J.4    Saska, I.5    Craik, D.J.6
  • 48
    • 0037424506 scopus 로고    scopus 로고
    • Twists, knots, and rings in proteins: Structural definition of the cyclotide framework
    • DOI 10.1074/jbc.M211147200
    • K.J. Rosengren, N.L. Daly, M.R. Plan, C. Waine, and D.J. Craik Twists, knots, and rings in proteins. Structural definition of the cyclotide framework The Journal of Biological Chemistry 278 2003 8606 8616 (Pubitemid 36800615)
    • (2003) Journal of Biological Chemistry , vol.278 , Issue.10 , pp. 8606-8616
    • Rosengren, K.J.1    Daly, N.L.2    Plan, M.R.3    Waine, C.4    Craik, D.J.5
  • 49
    • 0033990048 scopus 로고    scopus 로고
    • Primer3 on the WWW for general users and for biologist programmers
    • S. Misener, S.A. Krawetz, Humana Press Clifton
    • S. Rozen, and H. Skaletsky Primer3 on the WWW for general users and for biologist programmers S. Misener, S.A. Krawetz, Bioinformatics methods and protocols 132 1999 Humana Press Clifton 365 386
    • (1999) Bioinformatics Methods and Protocols , vol.132 , pp. 365-386
    • Rozen, S.1    Skaletsky, H.2
  • 50
    • 0028927655 scopus 로고
    • Elucidation of the primary and three-dimensional structure of the uterotonic polypeptide kalata B1
    • O. Saether, D.J. Craik, I.D. Campbell, K. Sletten, J. Juul, and D.G. Norman Elucidation of the primary and three-dimensional structure of the uterotonic polypeptide kalata B1 Biochemistry 34 1995 4147 4158
    • (1995) Biochemistry , vol.34 , pp. 4147-4158
    • Saether, O.1    Craik, D.J.2    Campbell, I.D.3    Sletten, K.4    Juul, J.5    Norman, D.G.6
  • 51
    • 84857388366 scopus 로고    scopus 로고
    • A synthetic mirror image of kalata B1 reveals that cyclotide activity is independent of a protein receptor
    • L. Sando, S.T. Henriques, F. Foley, S.M. Simonsen, N.L. Daly, and K.N. Hall A synthetic mirror image of kalata B1 reveals that cyclotide activity is independent of a protein receptor Chembiochem 12 2011 2456 2462
    • (2011) Chembiochem , vol.12 , pp. 2456-2462
    • Sando, L.1    Henriques, S.T.2    Foley, F.3    Simonsen, S.M.4    Daly, N.L.5    Hall, K.N.6
  • 53
    • 77953263435 scopus 로고    scopus 로고
    • The role of mass transport limitation and surface heterogeneity in the biophysical characterization of macromolecular binding processes by SPR biosensing
    • P. Schuck, and H. Zhao The role of mass transport limitation and surface heterogeneity in the biophysical characterization of macromolecular binding processes by SPR biosensing Methods in Molecular Biology 627 2010 15 54
    • (2010) Methods in Molecular Biology , vol.627 , pp. 15-54
    • Schuck, P.1    Zhao, H.2
  • 54
    • 33745227238 scopus 로고    scopus 로고
    • Conformation and mode of membrane interaction in cyclotides. Spatial structure of kalata B1 bound to a dodecylphosphocholine micelle
    • Z.O. Shenkarev, K.D. Nadezhdin, V.A. Sobol, A.G. Sobol, L. Skjeldal, and A.S. Arseniev Conformation and mode of membrane interaction in cyclotides. Spatial structure of kalata B1 bound to a dodecylphosphocholine micelle The FEBS Journal 273 2006 2658 2672
    • (2006) The FEBS Journal , vol.273 , pp. 2658-2672
    • Shenkarev, Z.O.1    Nadezhdin, K.D.2    Sobol, V.A.3    Sobol, A.G.4    Skjeldal, L.5    Arseniev, A.S.6
  • 55
    • 33645033202 scopus 로고    scopus 로고
    • A continent of plant defense peptide diversity: Cyclotides in Australian hybanthus (Violaceae)
    • S.M. Simonsen, L. Sando, D.C. Ireland, M.L. Colgrave, R. Bharathi, and U. Goransson A continent of plant defense peptide diversity: Cyclotides in Australian hybanthus (Violaceae) The Plant Cell 17 2005 3176 3189
    • (2005) The Plant Cell , vol.17 , pp. 3176-3189
    • Simonsen, S.M.1    Sando, L.2    Ireland, D.C.3    Colgrave, M.L.4    Bharathi, R.5    Goransson, U.6
  • 57
    • 0037088544 scopus 로고    scopus 로고
    • Refined structure and metal binding site of the kalata B1 peptide
    • DOI 10.1006/abbi.2002.2769
    • L. Skjeldal, L. Gran, K. Sletten, and B.F. Volkman Refined structure and metal binding site of the kalata B1 peptide Archives of Biochemistry and Biophysics 399 2002 142 148 (Pubitemid 34848600)
    • (2002) Archives of Biochemistry and Biophysics , vol.399 , Issue.2 , pp. 142-148
    • Skjeldal, L.1    Gran, L.2    Sletten, K.3    Volkman, B.F.4
  • 58
    • 0019304939 scopus 로고
    • Colorimetric determination of phospholipids with ammonium ferrothiocyanate
    • DOI 10.1016/0003-2697(80)90269-9
    • J.C. Stewart Colorimetric determination of phospholipids with ammonium ferrothiocyanate Analytical Biochemistry 104 1980 10 14 (Pubitemid 10045896)
    • (1980) Analytical Biochemistry , vol.104 , Issue.1 , pp. 10-14
    • Stewart, J.C.M.1
  • 60
    • 65949100618 scopus 로고    scopus 로고
    • Circular proteins from Melicytus (Violaceae) refine the conserved protein and gene architecture of cyclotides
    • M. Trabi, J.S. Mylne, L. Sando, and D.J. Craik Circular proteins from Melicytus (Violaceae) refine the conserved protein and gene architecture of cyclotides Organic & Biomolecular Chemistry 7 2009 2378 2388
    • (2009) Organic & Biomolecular Chemistry , vol.7 , pp. 2378-2388
    • Trabi, M.1    Mylne, J.S.2    Sando, L.3    Craik, D.J.4
  • 63
    • 58149291681 scopus 로고    scopus 로고
    • Identification of two suites of cyclotide precursor genes from metallophyte Viola baoshanensis: CDNA sequence variation, alternative RNA splicing and potential cyclotide diversity
    • J. Zhang, B. Liao, D.J. Craik, J.-T. Li, M. Hu, and W.-S. Shu Identification of two suites of cyclotide precursor genes from metallophyte Viola baoshanensis: cDNA sequence variation, alternative RNA splicing and potential cyclotide diversity Gene 431 2009 23 32
    • (2009) Gene , vol.431 , pp. 23-32
    • Zhang, J.1    Liao, B.2    Craik, D.J.3    Li, J.-T.4    Hu, M.5    Shu, W.-S.6


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