Optimized magnetic bead-based immunoassay for automated detection of protein toxins

Biochip Journal

Volumn 6, Issue 3, 2012, Pages 293-298

  Jung, Deok Ho ^a   Min, Kyoungmi ^a   Jeon, Yongkwon ^a   Jang, Wonhee ^a   Kwon, Youngeun ^a  

^a Dongguk University   (South Korea)

Author keywords

Autonomous detection; Fluorescent tag; Immunoassay; Magnetic bead; Toxins

Indexed keywords

ANTIBODIES; AUTOMATION; FLUORESCENCE; IMMUNOLOGY; TOXIC MATERIALS;

AUTONOMOUS DETECTION; FLUORESCENT TAG; IMMUNOASSAY; MAGNETIC BEADS; TOXINS;

CHEMICAL DETECTION;

IMMUNOGLOBULIN G ANTIBODY; OVALBUMIN; OVALBUMIN ANTIBODY; POLYCLONAL ANTIBODY; TOXIN; UNCLASSIFIED DRUG;

ARTICLE; AUTOMATION; CONCENTRATION (PARAMETERS); IMMUNOASSAY; LIMIT OF DETECTION; MAGNETIC AND ELECTROMAGNETIC EQUIPMENT; MAGNETIC BEAD; PROCESS OPTIMIZATION; REACTION TIME; SENSITIVITY ANALYSIS; TOXIN ANALYSIS;

EID: 84866975687     PISSN: 19760280     EISSN: 20927843     Source Type: Journal    
DOI: 10.1007/s13206-012-6312-3     Document Type: Article

References (19)

1. An, Y. R. et al. Analysis of toxicity of tetrabutyltin: comparing with EDC chemicals. Mol. Cell. Toxicol. 7, 95-101 (2011).
2. Yim, W. C. et al. Identification of novel 17β-estradiol (E2) target genes using cross-experiment gene expression datasets. Toxicol. Environ. Health Sci. 2, 25-38 (2010).
3. Ko, Y. J. et al. Field application of a recombinant protein-based ELISA during the 2010 outbreak of footand-mouth disease type A in South Korea. J. Virol. Methods179, 265-268 (2012).
4. Park, T. J. et al. Development of label-free optical diagnosis for sensitive detection of influenza virus with genetically engineered fusion protein. Talanta30, 246-252 (2012).
5. Kwon, Y. et al. Magnetic bead based immunoassay for autonomous detection of toxins. Anal. Chem. 80, 8416-8423 (2008).
6. Kang, J., Kim, S. & Kwon, Y. Antibody based biosensors for environmental monitoring. Toxicol. Environ. Health Sci. 1, 145-150 (2009).
7. McBride, M. T. et al. Autonomous detection of aerosolized Bacillus anthracis and Yersinia pestis. Anal. Chem. 75, 5293-5299 (2003).
8. Hindson, B. J. et al. Development of an automated sample preparation module for environmental monitoring of biowarfare agents. Anal. Chem. 76, 3492-3497 (2004).
9. Hindson, B. J. et al. Autonomous detection of aerosolized biological agents by multiplexed immunoassay with polymerase chain reaction confirmation. Anal. Chem. 77, 284-289 (2005).
10. Yim, W. C., Min, K., Jung, D., Lee, B. M. & Kwon, Y. Cross experimental analysis of microarray gene expression data from volatile organic compounds treated targets. Mol. Cell. Toxicol. 7, 233-241 (2011).
11. Chan-Hui, P. Y., Stephens, K., Warnock, R. A. & Singh, S. Applications of eTag trade mark assay platform to systems biology approaches in molecular oncology and toxicology studies. Clin. Immunol. 111, 162-174 (2004).
12. 2+ and bovine serum albumin. Dalton Trans14, 9737-9745 (2011).
13. Zeni, O. & Scarf, M. R. DNA damage by carbon nanotubes using the single cell gel electrophoresis technique. Methods Mol. Biol. 625, 109-119 (2010).
14. Walczak, R. et al. Fluorescence detection by miniaturized instrumentation based on non-cooled CCD minicamera and dedicated for lab-on-a-chip applications. BioChip J. 5, 271-279 (2011).
15. Kwon, Y., Han, Z., Karatan, E., Mrksich, M. & Kay, B. Antibody arrays prepared by cutinase-mediated immobilization on self-assembled monolayers. Anal. Chem. 76, 5713-5720 (2004).
16. Lionnet, T. et al. Magnetic trap construction. Cold Spring Harb. Protoc. 1, 133-138 (2012).
17. Pai, N. P. & Pai, M. Point-of-care diagnostics for HIV and tuberculosis: landscape, pipeline, and unmet needs. Discov. Med. 13, 35-45 (2012).
18. Friedman, E. S. et al. A cost-effective and field-ready potentiostat that poises subsurface electrodes to monitor bacterial respiration. Biosens. Bioelectron. 32, 309-313 (2012).
19. Inglis, T. J. et al. Deployable laboratory response to influenza pandemic; PCR assay field trials and comparison with reference methods. PLoS One. 6, e25526 (2011).