A coincidence reporter-gene system for high-throughput screening

Nature Methods

Volumn 9, Issue 10, 2012, Pages 937-

  Cheng, Ken C C ^a   Inglese, James ^a,b  

^a NATIONAL INSTITUTES OF HEALTH   (United States)

^b NATIONAL HUMAN GENOME RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYCLIC AMP; FIREFLY LUCIFERASE; FORSKOLIN;

BIOLOGICAL ACTIVITY; CATALYSIS; CELL STRAIN HEK293; CONCENTRATION (PARAMETERS); ENZYME ACTIVITY; GENE EXPRESSION; HIGH THROUGHPUT SCREENING; INHIBITION KINETICS; LETTER; PRIORITY JOURNAL; REPORTER GENE; SIGNAL TRANSDUCTION; STOICHIOMETRY;

GENES, REPORTER; HEK293 CELLS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; LUCIFERASES;

EID: 84866974569     PISSN: 15487091     EISSN: 15487105     Source Type: Journal    
DOI: 10.1038/nmeth.2170     Document Type: Letter

References (5)

1. Michelini, E., Cevenini, L., Mezzanotte, L., Coppa, A. & Roda. A. Anal. Bioanal. Chem. 398, 227-238 (2010).
2. Lyssiotis, C.A. et al. Proc. Natl. Acad. Sci. USA 106, 8912-8917 (2009).
3. de Felipe, P. et al. Trends Biotechnol. 24, 68-75 (2006).
4. Thorne, N., Inglese, J. & Auld, D.S. Chem. Biol. 17, 646-657 (2010).
5. Mundell, S.J. & Benovic, J.L. J. Biol. Chem. 275, 12900-12908 (2000).