Evaluation of in-house mpt64 real-time PCR for rapid detection of Mycobacterium tuberculosis in pulmonary and extra-pulmonary specimens

Brazilian Journal of Infectious Diseases

Volumn 16, Issue 5, 2012, Pages 493-494

  Sethi, Sunil ^a   Yadav, Rakesh ^a   Mewara, Abhishek ^a   Dhatwalia, Sunil Kumar ^a   Sharma, Meera ^a   Gupta, Dheeraj ^a  

^a POSTGRADUATE INSTITUTE OF MEDICAL EDUCATION AND RESEARCH   (India)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN;

ACCURACY; BACTERIAL GENE; BACTERIUM CULTURE; BACTERIUM DETECTION; CLINICAL EVALUATION; CONTROLLED STUDY; EARLY DIAGNOSIS; EXTRAPULMONARY TUBERCULOSIS; GENE TARGETING; HUMAN; HUMAN TISSUE; LETTER; LUNG PARENCHYMA; MAJOR CLINICAL STUDY; MOLECULAR DIAGNOSIS; MPT64 GENE; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PREDICTIVE VALUE; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TISSUE SECTION;

CASE-CONTROL STUDIES; DNA, BACTERIAL; HUMANS; MYCOBACTERIUM TUBERCULOSIS; PREDICTIVE VALUE OF TESTS; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; TUBERCULOSIS;

EID: 84866882851     PISSN: 14138670     EISSN: 16784391     Source Type: Journal    
DOI: 10.1016/j.bjid.2012.08.010     Document Type: Letter

References (7)

1. Moatter T., Mirza S., Siddiqui M.S., Soomro I.N. Detection of Mycobacterium tuberculosis in paraffin embedded intestinal tissue specimens by polymerase chain reaction: characterization of IS6110 element negative strains. J Pak Med Assoc 1998, 48:174-178.
2. Purohit M.R., Mustafa T., Wiker G.H., Morkve O., Sviland L. Immunohistochemical diagnosis of abdominal and lymph node tuberculosis by detecting Mycobacterium tuberculosis complex specific antigen MPT64. Diagn Pathol 2007, 2:36.
3. Bhanu N.V., Singh U.B., Chakraborty M., et al. Improved diagnostic value of PCR in the diagnosis of female genital tuberculosis leading to infertility. J Med Microbiol 2005, 54:927-931.
4. Singh U.B., Bhanu N.V., Suresh V.N., Arora J., Rana T., Seth P. Utility of polymerase chain reaction in diagnosis of tuberculosis from samples of bone marrow aspirate. Am J Trop Med Hyg 2006, 75:960-963.
5. Eisenach K.D., Cave M.D., Bates J.H., Crawford J.T. Polymerase chain reaction amplification of repetitive DNA sequence specific for Mycobacterium tuberculosis. J Infect Dis 1990, 161:977-981.
6. Das S.D., Narayanan S., Hari L., et al. Differentiation of highly prevalent IS6110 single-copy strains of Mycobacterium tuberculosis from rural community in South India with an ongoing DOTS programme. Infect Genet Evol 2005, 5:67-77.
7. Siddiqi N., Shamim M., Jain N.K., et al. Molecular genetic analysis of multi-drug resistance in Indian isolates of Mycobacterium tuberculosis. Mem Inst Oswaldo Cruz 1998, 93:589-594.