Standardization of the Teratoma Assay for Analysis of Pluripotency of Human ES Cells and Biosafety of Their Differentiated Progeny

PLoS ONE

Volumn 7, Issue 9, 2012, Pages

  Gropp, Michal ^a   Shilo, Vitali ^b   Vainer, Gilad ^a   Gov, Miri ^b   Gil, Yaniv ^a   Khaner, Hanita ^a   Matzrafi, Limor ^b   Idelson, Maria ^a   Kopolovic, Juri ^a   Zak, Naomi B ^b   Reubinoff, Benjamin E ^a  

^a HADASSAH HEBREW UNIVERSITY MEDICAL CENTER   (Israel)

^b CellCure Neurosciences Ltd   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BIOSAFETY; CARCINOGENESIS; CELL ASSAY; CELL DIFFERENTIATION; CELL POPULATION; CONTROLLED STUDY; EMBRYO; EMBRYONIC STEM CELL; FEEDER CELL; HUMAN; HUMAN CELL; HUMAN TISSUE; MOUSE; NONHUMAN; PLURIPOTENT STEM CELL; PROGENY; QUANTITATIVE ASSAY; REPRODUCIBILITY; SENSITIVITY ANALYSIS; STANDARDIZATION; STEM CELL TRANSPLANTATION; TERATOMA;

ANIMALS; BIOLOGICAL ASSAY; BIOLOGICAL MARKERS; CELL COUNT; CELL DIFFERENTIATION; COLLAGEN; DRUG COMBINATIONS; EMBRYONIC STEM CELLS; FEEDER CELLS; FIBROBLASTS; HUMANS; INJECTIONS, SUBCUTANEOUS; KARYOTYPING; LAMININ; MICE; MICE, INBRED NOD; MICE, SCID; PLURIPOTENT STEM CELLS; PROTEOGLYCANS; SENSITIVITY AND SPECIFICITY; SURVIVAL RATE; TERATOMA;

ANIMALIA; MUS;

EID: 84866678032     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0045532     Document Type: Article

References (29)

1. Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, et al. (1998) Embryonic stem cell lines derived from human blastocysts. Science 282: 1145-1147.
2. Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A, (2000) Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro. Nat Biotechnol 18: 399-404.
3. Lensch MW, Ince TA, (2007) The terminology of teratocarcinomas and teratomas. Nat Biotechnol 25: 1211.
4. Blum B, Benvenisty N, (2008) The tumorigenicity of human embryonic stem cells. Adv Cancer Res 100: 133-158.
5. Brivanlou AH, Gage FH, Jaenisch R, Jessell T, Melton D, et al. (2003) Stem cells. Setting standards for human embryonic stem cells. Science 300: 913-916.
6. Consensus guidance for banking and supply of human embryonic stem cell lines for research purposes. Stem Cell Rev 5: 301-314.
7. Maherali N, Hochedlinger K, (2008) Guidelines and techniques for the generation of induced pluripotent stem cells. Cell Stem Cell 3: 595-605.
8. Muller FJ, Goldmann J, Loser P, Loring JF, (2010) A call to standardize teratoma assays used to define human pluripotent cell lines. Cell Stem Cell 6: 412-414.
9. Mayor S, (2010) First patient enters trial to test safety of stem cells in spinal injury. Bmj 341: c5724.
10. Werbowetski-Ogilvie TE, Bosse M, Stewart M, Schnerch A, Ramos-Mejia V, et al. (2009) Characterization of human embryonic stem cells with features of neoplastic progression. Nat Biotechnol 27: 91-97.
11. Lee AS, Tang C, Cao F, Xie X, van der Bogt K, et al. (2009) Effects of cell number on teratoma formation by human embryonic stem cells. Cell Cycle 8: 2608-2612.
12. Shih CC, Forman SJ, Chu P, Slovak M, (2007) Human embryonic stem cells are prone to generate primitive, undifferentiated tumors in engrafted human fetal tissues in severe combined immunodeficient mice. Stem Cells Dev 16: 893-902.
13. Hentze H, Soong PL, Wang ST, Phillips BW, Putti TC, et al. (2009) Teratoma formation by human embryonic stem cells: Evaluation of essential parameters for future safety studies. Stem Cell Res 2: 198-210.
14. Prokhorova TA, Harkness LM, Frandsen U, Ditzel N, Burns JS, et al. (2008) Teratoma Formation by Human Embryonic Stem Cells is site-dependent and enhanced by the presence of Matrigel. Stem Cells Dev 18: 47-54.
15. Drukker M, Katchman H, Katz G, Even-Tov Friedman S, Shezen E, et al. (2006) Human embryonic stem cells and their differentiated derivatives are less susceptible to immune rejection than adult cells. Stem Cells 24: 221-229.
16. Watanabe K, Ueno M, Kamiya D, Nishiyama A, Matsumura M, et al. (2007) A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotechnol 25: 681-686.
17. Cohen MA, Itsykson P, Reubinoff BE (2007) Neural differentiation of human ES cells. Curr Protoc Cell Biol Chapter 23: Unit 23 27.
18. Idelson M, Alper R, Obolensky A, Ben-Shushan E, Hemo I, et al. (2009) Directed differentiation of human embryonic stem cells into functional retinal pigment epithelium cells. Cell Stem Cell 5: 396-408.
19. Chang Y, Finnemann SC, (2007) Tetraspanin CD81 is required for the alpha v beta5-integrin-dependent particle-binding step of RPE phagocytosis. J Cell Sci 120: 3053-3063.
20. Daley GQ, Lensch MW, Jaenisch R, Meissner A, Plath K, et al. (2009) Broader implications of defining standards for the pluripotency of iPSCs. Cell Stem Cell 4: 200-201; author reply 202.
21. Draper JS, Smith K, Gokhale P, Moore HD, Maltby E, et al. (2004) Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells. Nat Biotechnol 22: 53-54.
22. Baker DE, Harrison NJ, Maltby E, Smith K, Moore HD, et al. (2007) Adaptation to culture of human embryonic stem cells and oncogenesis in vivo. Nat Biotechnol 25: 207-215.
23. Spits C, Mateizel I, Geens M, Mertzanidou A, Staessen C, et al. (2008) Recurrent chromosomal abnormalities in human embryonic stem cells. Nat Biotechnol 26: 1361-1363.
24. Blum B, Benvenisty N, (2009) The tumorigenicity of diploid and aneuploid human pluripotent stem cells. Cell Cycle 8: 3822-3830.
25. Andrews PW, Matin MM, Bahrami AR, Damjanov I, Gokhale P, et al. (2005) Embryonic stem (ES) cells and embryonal carcinoma (EC) cells: opposite sides of the same coin. Biochem Soc Trans 33: 1526-1530.
26. Lawrenz B, Schiller H, Willbold E, Ruediger M, Muhs A, et al. (2004) Highly sensitive biosafety model for stem-cell-derived grafts. Cytotherapy 6: 212-222.
27. Fink DW Jr, (2009) FDA regulation of stem cell-based products. Science 324: 1662-1663.
28. Goldring CE, Duffy PA, Benvenisty N, Andrews PW, Ben-David U,et al. Assessing the safety of stem cell therapeutics. Cell Stem Cell 8: 618-628.
29. Ben-Dor I, Itsykson P, Goldenberg D, Galun E, Reubinoff BE, (2006) Lentiviral vectors harboring a dual-gene system allow high and homogeneous transgene expression in selected polyclonal human embryonic stem cells. Mol Ther 14: 255-267.