Simultaneous high-performance liquid chromatography determination of coenzyme A, dephospho-coenzyme A, and acetyl-coenzyme A in normal and pantothenic acid-deficient rats

Analytical Biochemistry

Volumn 430, Issue 2, 2012, Pages 151-155

  Shibata, Katsumi ^a   Nakai, Takumi ^a   Fukuwatari, Tsutomu ^a  

^a UNIVERSITY OF SHIGA PREFECTURE   (Japan)

Author keywords

Acetyl coenzyme A; Coenzyme A; Deficiency; Dephospho coenzyme A; HPLC; Pantothenic acid

Indexed keywords

FATTY ACIDS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HISTOLOGY; METABOLISM; PARTICLE SIZE; PARTICLE SIZE ANALYSIS; RATS; SODIUM COMPOUNDS; TISSUE; TISSUE ENGINEERING;

ACETYL COENZYME A; COENZYME A; DEFICIENCY; HPLC; PANTOTHENIC ACID;

COENZYMES;

ACETONITRILE; ACETYL COENZYME A; COENZYME A; DEPHOSPHO COENZYME A; PANTOTHENIC ACID; UNCLASSIFIED DRUG;

AMINO ACID METABOLISM; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BODY WEIGHT; COLON; CONTROLLED STUDY; DIETARY INTAKE; ENZYME ASSAY; ENZYME BLOOD LEVEL; ENZYME ISOLATION; ENZYME STABILITY; ENZYME SYNTHESIS; FATTY ACID METABOLISM; FLOW RATE; HEART; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; KIDNEY PARENCHYMA; LIVER; MALE; MUSCLE TISSUE; NONHUMAN; NUTRIENT; ORGAN WEIGHT; PAPER CHROMATOGRAPHY; PARTICLE SIZE; PRIORITY JOURNAL; PROTEIN URINE LEVEL; RADIATION DETECTOR; RAT; SPLEEN; TESTIS; TISSUE LEVEL; VITAMIN DEFICIENCY;

PAA; RATTUS;

EID: 84866566068     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.08.010     Document Type: Article

References (10)

1. K. Shibata, C.J. Gross, and L.M. Henderson Hydrolysis and absorption of pantothenate and its coenzymes in the rat small intestine J. Nutr. 113 1983 247 265
2. K. Takahashi, T. Fukuwatari, and K. Shibata Fluorometric determination of pantothenic acid in human urine by isocratic reversed-phase ion-pair high-performance liquid chromatography with post-column derivatization J. Chromatogr. 877 2009 2168 2172
3. H.R. Skeggs, and L.D. Wright The use of Lactobacillus arabinosus in the microbiological determination of pantothenic acid J. Biol. Chem. 156 1944 21 26
4. B.E. Corkey, M. Brandt, R.J. Williams, and J.R. Williamson Assay of short-chain acyl coenzyme A intermediates in tissue extracts by high-pressure liquid chromatography Anal. Biochem. 118 1981 30 41
5. K. Bartlett, and A.G. Causey Radiochemical high-performance liquid chromatography methods for the study of branched-chain amino acid metabolism Methods Enzymol. 166 1988 79 92
6. A. Demoz, A. Garras, D.K. Asiedu, B. Netteland, and R.K. Berge Rapid method for the separation and detection of tissue short-chain coenzyme A esters by reversed-phase high-performance liquid chromatography J. Chromatogr. 667 1995 148 152
7. P.G. Reeves Components of the AIN-93 diets as improvements in the AIN-76A diet J. Nutr. 127 1997 838S 841S
8. T. Nakamura, T. Kusunoki, H. Yoyama, and N. Kuwagata Studies on the distribution of pantothenic acid, coenzyme A, and their intermediates in rat liver: III. The isolation of pantothenic acid, 4′-phosphopantetheine, and coenzyme A by using column chromatography Vitamins 40 1969 412 415 (in Japanese)
9. T. Nakamura, T. Kusunoki, H. Yoyama, K. Tsuji, and K. Tanaka Studies on the distribution of pantothenic acid, coenzyme A, and their intermediates in rat liver II: the effect of various amounts of dietary pantothenic acid upon the distribution of pantothenic acid, coenzyme A, and their intermediates and the activity of 4′-phosphopantothenoylcysteine decarboxylase in rat liver Vitamins 40 1969 354 357 (in Japanese)
10. D.K. Reibel, B.W. Wyse, D.A. Berkich, and J.B. Neely Coenzyme A metabolism in pantothenic acid-deficient rats J. Nutr. 112 1982 1144 1150