Analysis of protein ubiquitination

Current Protocols in Protein Science

Volumn 1, Issue SUPPL.66, 2011, Pages

  Laney, Jeffrey D ^a   Hochstrasser, Mark ^b  

^a BROWN UNIVERSITY   (United States)

^b YALE UNIVERSITY   (United States)

Author keywords

Immunoprecipitation; Ubiquitin; Ubiquitin protein ligase

Indexed keywords

BUFFER; GUANIDINE; HEXAHISTIDINE; PROTEIN ANTIBODY; PROTEINASE INHIBITOR; REAGENT; SODIUM; TYLOXAPOL; UBIQUITIN; UBIQUITIN ANTIBODY; UBIQUITIN PROTEIN LIGASE; UNCLASSIFIED DRUG; UREA;

ARTICLE; BINDING AFFINITY; CHEMICAL MODEL; CLINICAL PROTOCOL; ESCHERICHIA COLI; EXPERIMENTAL DESIGN; IMMUNOBLOTTING; IMMUNOPRECIPITATION; IN VITRO STUDY; LYSIS; MAMMAL CELL; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROCESS DESIGN; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN TARGETING; PROTEIN TRANSPORT; UBIQUITINATION; YEAST CELL;

EUKARYOTA; MAMMALIA;

EID: 84866565589     PISSN: 19343655     EISSN: 19343663     Source Type: Journal    
DOI: 10.1002/0471140864.ps1405s66     Document Type: Article

References (26)

1. Al-Hakim, A., Escribano-Diaz, C., Landry, M.C., ODonnell, L., Panier, S., Szilard, R.K., and Durocher, D. 2010. The ubiquitous role of ubiquitin in the DNA damage response. DNA Repair 9:1229-1240.
2. Ardley, H.C. and Robinson, P.A. 2005. E3 ubiquitin ligases. Essays Biochem. 41:15-30.
3. Bhat, K.P. and Greer, S.F. 2011. Proteolytic and non-proteolytic roles of ubiquitin and the ubiquitin proteasome system in transcriptional regulation. Biochim. Biophys. Acta 1809:150-155.
4. Chau, V., Tobias, J.W., Bachmair, A., Marriott, D., Ecker, D.J., Gonda, D.K., and Varshavsky, A. 1989. A multiubiquitin chain is confined to specific lysine in a targeted short-lived protein. Science 243:1576-1583.
5. Ciechanover, A., Finley, D., and Varshavsky, A. 1984. Ubiquitin dependence of selective protein degradation demonstrated in the mammalian cell cycle mutant ts85. Cell 37:57-66.
6. Clague, M.J. and Urbe, S. 2010. Ubiquitin: same molecule, different degradation pathways. Cell 143:682-685.
7. Deshaies, R.J. and Joazeiro, C.A. 2009. RING domain E3 ubiquitin ligases. Annu. Rev. Biochem. 78:399-434.
8. Finley, D., Sadis, S., Monia, B., Boucher, P., Ecker, D., Crooke, S., and Chau, V. 1994. Inhibition of proteolysis and cell cycle progression in a multiubiquitination-deficient yeast mutant. Mol. Cell Biol. 14:5501-5509.
9. Ghaboosi, N. and Deshaies, R.J. 2007. A conditional yeast E1 mutant blocks the ubiquitinproteasome pathway and reveals a role for ubiquitin conjugates in targeting Rad23 to the proteasome. Mol. Biol. Cell 18:1953-1963.
10. Hershko, A. and Ciechanover, A. 1998. The ubiquitin system. Annu. Rev. Biochem. 67:425-479.
11. Hershko, A., Heller, H., Elias, S., and Ciechanover, A. 1983. Components of ubiquitin-protein ligase system: resolution, affinity purification, and role in protein breakdown. J. Biol. Chem. 258:8206-8214.
12. Hislop, J.N. and von Zastrow, M. 2011. Role of ubiquitination in endocytic trafficking of G-protein-coupled receptors. Traffic 12:137-148.
13. Hodgins, R.R.W., Ellison, K.S., and Ellison, M.J. 1992. Expression of a ubiquitin derivative that conjugates to protein irreversibly produces phenotypes consistent with a ubiquitin deficiency. J. Biol. Chem. 267:8807-8812.
14. Ikeda, F. and Dikic, I. 2008. Atypical ubiquitin chains: New molecular signals. Protein Modifications: Beyond the usual suspects review series. EMBO Rep. 9:536-542.
15. Iwai, K. 2010. Functions of linear ubiquitin chains in the NF-κB pathway: Linear polyubiquitin in NF-κB signaling. Subcell. Biochem. 54:100-106.
16. Kaiser, P., Flick, K., Wittenberg, C., and Reed, S.I. 2000. Regulation of transcription by ubiquitination without proteolysis: Cdc34/SCF(Met30)-mediated inactivation of the transcription factor Met4. Cell 102:303-314.
17. King, R.W., Peters, J.M., Tugendreich, S., Rolfe, M., Hieter, P., and Kirschner, M.W. 1995. A 20S complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B. Cell 81:279-288.
18. Petroski, M.D. and Deshaies, R.J. 2005. Mechanism of lysine 48-linked ubiquitin-chain synthesis by the cullin-RING ubiquitin-ligase complex SCFCdc34. Cell 123:1107-1120.
19. Pierce, N.W., Kleiger, G., Shan, S.O., and Deshaies, R.J. 2009. Detection of sequential polyubiquitylation on a millisecond timescale. Nature 462:615-619.
20. Scheffner, M., Huibregste, J.M., Vierstra, R.D., and Howley, P.M. 1993. The HPV E6 and E6-AP complex functions as a ubiquitin-protein ligase in the ubiquitination of p53. Cell 75:495-505.
21. Spencer, E., Jiang, J., and Chen, Z.J. 1999. Signalinduced ubiquitination of IkappaBalpha by the F-box protein Slimb/beta-TrCP. Genes Dev. 13:284-294.
22. Swaminathan, S., Amerik, A.Y., and Hochstrasser, M. 1999. The Doa4 deubiquitinating enzyme is required for ubiquitin homeostasis in yeast. Mol. Biol. Cell 10:2583-2594.
23. Treier, M., Staszewski, L.M., and Bohmann, D. 1994. Ubiquitin-dependent c-jun degradation in vivo is mediated by the δ domain. Cell 78:787-798.
24. Vembar, S.S. and Brodsky, J.L. 2008. One step at a time: endoplasmic reticulum-associated degradation. Nat. Rev. Mol. Cell. Biol. 9:944-957.
25. Verma, R., Chi, Y., and Deshaies, R.J. 1997. Cell-free ubiquitination of cell cycle regulators in budding yeast extracts. Methods Enzymol. 283:366-376.
26. Xu, P., Duong, D.M., Seyfried, N.T., Cheng, D., Xie, Y., Robert, J., Rush, J., Hochstrasser, M., Finley, D., and Peng, J. 2009. Quantitative proteomics reveals the function of unconventional ubiquitin chains in proteasomal degradation. Cell 137:133-145