Production of polyhydroxybutyrate (PHB) by Alcaligenes latus using sugarbeet juice

Industrial Crops and Products

Volumn 43, Issue 1, 2013, Pages 802-811

  Wang, Bingqing ^a   Sharma Shivappa, Ratna R ^a   Olson, Jonathan W ^b   Khan, Saad A ^c  

^a North Carolina State University   (United States)

^b NORTH CAROLINA STATE UNIVERSITY   (United States)

^c North Carolina State University   (United States)

Author keywords

Biodegradable plastics; Polyhydroxyalkanoate (PHA); Sucrose; Two stage batch fermentation

Indexed keywords

ALCALIGENES LATUS; BATCH FERMENTATION; BIODEGRADABLE PLASTICS; CRYSTALLINITIES; CULTURE MEDIA; DRY CELLS; NUTRIENT ADDITION; PHB PRODUCTION; POLYHYDROXYALKANOATES; POLYHYDROXYBUTYRATE;

BIODEGRADABLE POLYMERS; BIOLOGICAL MATERIALS; CELL CULTURE; SUGAR (SUCROSE);

NUTRIENTS;

BACTERIUM; BIODEGRADATION; CRYSTALLINITY; EXPERIMENTAL STUDY; FERMENTATION; PLASTIC; SUGAR; SUGAR BEET; TEMPERATURE EFFECT;

ALCALIGENES LATUS;

EID: 84866491809     PISSN: 09266690     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.indcrop.2012.08.011     Document Type: Article

References (45)

1. Aremu M.O., Olu-Arotiowa O.A., Layokun S.K., Solomon B.O. Growth of Pseudomonas Fluorescens on cassava starch hydroltsate for polyhydroxybutyrate production. J. Appl. Sci. Environ. Manage. 2010, 14(4):61-66.
2. Barham P.G., Keller A., Otum E.L., Holmes A. Crystallization and morphology of a bacterial thermoplastic: poly-3-hydroxybutyrate. J. Mater. Sci. 1984, 19:2781-2794.
3. Braunegg G., Bogensberger B. Zur Kinetik des Wachstums und der Speicherungvon poly-d(-)-3-hydroxybuttersaure by Alcaligenes latus. Acta Biotechnol. 1985, 5:339-345.
4. Bonino C.A., Samorezov J.E., Jeon O., Alsberg E., Khan S.A. Real-time in situ rheology of alginate hydrogel photocrosslinking. Soft Matter 2011, 7:11510-11517.
5. Bruce W.Z. Wine Analysis and Production 1995, Chapman and Hall, p. 72.
6. Choi J., Lee S.Y. Factors affecting the economics of polyhydroxyalkanoate production by bacterial fermentation. Appl. Microbiol. Biotechnol. 1999, 51:13-21.
7. Clesceri L.S., Greenberg A.E., Eaton A.D. Standard Methods for the Examination of Water and Wastewater 1998, American Public Health Association, American Water Works Association, Water Environment Federation Press, Baltimore, MD. 20th ed.
8. El-Hadi A., Schnabel R., Straube E., Muller G., Riemschneider M. Effect of melt processing on crytallization behavior and rheology of poly(3-hydroxybutyrate) (PHB) and its blends. Macromol. Mater. Eng. 2002, 287:363-372.
9. El-Sayed A.A., Abdel Hafez A.M., Hemmat Abdelhady M., Khodair T.A. Production of polyhydroxybutyrate (PHB) using batch and two-stage batch culture strategies. Aust. J. Basic Appl. Sci. 2009, 3(2):617-627.
10. FAO Statistics, 2009. The Agricultural Production Domain. Available at: (accessed 01.11.11). http://faostat.fao.org/site/567/default.aspx.
11. Gomez J.G.C., Rodrigues M.F.A., Alli R.C.P., Torres B.B., Netto C.L.B., Oliveira M.S., Silva L.F. Evaluation of soil gram-negative bacteria yielding polyhydroxyalkanoic acids from carbohydrates and propionic acid. Appl. Microbiol. Biotechnol. 1996, 45:785-791.
12. Gunasekaran S., Mehmet A.K.M. Cheese Rheology and Texture 2003, CRC Press LLC, USA, pp. 226-228.
13. Greenberg A.E., Clesceri L.S., Eaton A.D. Standard Methods for the Examination of Water and Wastewater 2005, APHA, AWWA and WEF. 21st ed.
14. Grothe E., Moo-Young M., Chisti Y. Fermentation optimization for production of poly(β-hydroxybutyric acid) microbial thermoplastic. Enzyme Microb. Technol. 1999, 25:132-141.
15. Holt J., Krieg N., Sneath P., Stealey J., Williams S. Bergey's Manual of Determinative Bacteriology 1994, Williams & Wilkins, Baltimore, MD. 9th ed.
16. Huang T.Y., Duan K.J., Huang S.Y. Production of polyhydroxyalkanoates from inexpensive extruded rice bran and starch by Haloferax mediterranei. J. Microbiol. Biotechnol. 2006, 33:701-706.
17. Khanna S., Srivastava A.K. Statistical media optimization studies for growth and PHB production by Ralstonia eutropha. Process Biochem. 2005, 40:2173-2182.
18. Khardenavis A.A., Kumar M.S., Mudliar S.N., Chakrabarti T. Biotechnological conversion of agro-industrial wastewaters into biodegradable plastic, poly-β-hydroxybutyrate. Bioresour. Technol. 2007, 98:3579-3584.
19. Kim B.S., Lee S.C., Le S.Y., Chang H.N., Chang Y.K., Woo S.I. Production of poly(3-hydroxybutyric acid) by fed-batch culture of Alcaligenes eutrophus with glucose concentration control. Biotechnol. Bioeng. 1994, 43:892-898.
20. Lee S.Y. Plastic bacteria-progress and prospects for polyhydroxyalkanoate production in bacteria. Trends Biotechnol. 1996, 14:431-438.
21. Lunt J. Polylactic acid polymers from corn. Applications in the textiles industry. J. Ind. Text. 2000, 29(3):191-198.
22. NASS - Statistics by Subject - Crops and Plants - Field Crops - Sugarbeets. Available at: (accessed 18.07.10). http://www.nass.usda.gov/QuickStats/index2.jsp.
23. Organisation for Economic Co-operation and Development (OECD) Consensus Document on Compositional Considerations for New Varieties of Sugarbeet: Key Food and Feed Nutrients and Anti-Nutrients 2002, OECD Environmental Health and Safety Publications, Paris, Frankreich.
24. Ostle A.G., Holt J.G. Nile blue A as a fluorescent stain for poly-β-hydroxybuytrate. Appl. Environ. Microbiol. 1982, 44(1):238-241.
25. Page W.J. Production of poly-p-hydroxybutyrate by Azotobacter vinelandii strain UWD during growth on molasses and other complex carbon sources. Appl. Microbiol. Biotechnol. 1989, 31:329-333.
26. Page W.J. Production of polyhydroxyalkanoates by Azotobacter vinelandii UWD in beet molasses culture. FEMS Microbiol. Rev. 1992, 103:149-158.
27. Palleroni N.J., Palleroni A.V. Alcaligenes latus, a new species of hydrogen-utilizing bacteria. Int. J. Syst. Bacteriol. 1978, 28:416-424.
28. Patwardhan P.R., Srivastava A.K. Model-based fed-batch cultivation of R. eutropha for enhanced biopolymer production. Biochem. Eng. J. 2004, 20:21-28.
29. Raghavan S.R., Walls H.J., Khan S.A. Rheology of silica dispersions in organic liquids: new evidence for solvation forces dictated by hydrogen bonding. Langmuir 2000, 16(21):7920-7930.
30. Riis V., Mai W. Gas chromatographic determination of poly-β-hydroxybutyric acid in microbial biomass after hydrochloric acid propanolysis. J. Chromatogr. 1988, 445:285-289.
31. Santhanam A., Sasidharan S. Microbial production of polyhydroxy alkanotes (PHA) from Alcaligens spp. and Pseudomonas oleovorans using different carbon sources. Afr. J. Biotechnol. 2010, 9(21):3144-3150.
32. Tanamool V., Danvirutai P., Thanonkeo P., Imai T., Kaewkannetra P. Production of poly-β-hydroxybutyric acid (PHB) from sweet sorghum juice by Alcaligenes eutrophus TISTR 1095 and Alcaligenes latus ATCC 29714 via batch fermentation. The 3th International Conference on Fermentation Technology for Value Added Agroculture Products 2009.
33. USDA, 1981. Sucrose conversion table. File code 135-A-50.
34. USDA, 2009. Sugar and Sweeteners: Background Economic Research Service (ERS). Available at (accessed 10.11.10). http://www.ers.usda.gov/briefing/sugar/background.htm.
35. USDA, 2011. Agricultural Prices. Available at: (accessed 10.11.10). http://usda01.library.cornell.edu/usda/current/AgriPric/AgriPric-10-31-2011.txt.
36. USDA, 2011. Sugar and Sweetners: Yearbook Tables. Table 12. Available at: (accessed 01.11.11). http://www.ers.usda.gov/briefing/sugar/data.htm%23yearbook.
37. Wang B., Sharma-Shivappa R.R., Olson J.W., Khan S.A. Upstream process optimization of polyhydroxybutyrate (PHB) by Alcaligenes latus using two-stage batch and fed-batch fermentation strategies. Bioprocess Biosyst. Eng. 2012, 10.1007/s00449-012-0749-6.
38. Wang F., Lee S.Y. Poly(3-hydroxybutyrate) production with high productivity and high polymer content by a fed-batch culture of Alcaligenes latus under nitrogen limitation. Appl. Environ. Microbiol. 1997, 63(9):3703-3706.
39. Wegen R.J., Ling Y., Middelberg A.P.J. Industrial production of polyhydroxyalkanoates using Escherichia coli: an economic analysis. Trans. IChemE 1998, 76:417.
40. Yamane T., Fukunage M., Lee Y.M. Increased PHB productivity by high-cell-density fed-batch culture of Alcaligenes latus, a growth-associated PHB producer. Biotechnol. Bioeng. 1996, 50:197-202.
41. Yang S.T., Zhu H., Li Y., Hong G. Continuous propionate production from whey permeate using a novel fibrous bed bioreactor. Biotechnol. Bioeng. 1994, 43:1124-1130.
42. Yezza A., Halasz A., Levadoux W., Hawari J. Production of poly-β-hydroxybutyrate (PHB) by Alcaligenes latus from maple sap. Appl. Microbiol. Biotechnol. 2007, 77:269-274.
43. Yu P.H., Chua H., Huang A.L., Lo W., Chen G.Q. Conversion of food industrial wastes into bio-plastics. Appl. Microbiol. Biotechnol. 1998, 70-72:603-614.
44. Yu P.H., Chua H., Huang A.L., Ho K.P. Conversion of food industrial wastes by Alcaligenes latus into polyhydroxyalkanoates. Appl. Biochem. Biotechnol. 1999, 77-79:445-454.
45. Yu J. Production of PHA from starchy waste water via organic acids. J. Biotechnol. 2001, 86:105-112.