Human UDP-glucuronosyltransferase expression in insect cells: Ratio of active to inactive recombinant proteins and the effects of a C-terminal his-tag on glucuronidation kinetics

Drug Metabolism and Disposition

Volumn 40, Issue 10, 2012, Pages 1935-1944

  Zhang, Hongbo ^a   Patana, Anne Sisko ^b,e   Mackenzie, Peter I ^c   Ikushiro, Shinichi ^d   Goldman, Adrian ^b   Finel, Moshe ^a  

^a UNIVERSITY OF HELSINKI   (Finland)

^b UNIVERSITY OF HELSINKI   (Finland)

^c FLINDERS UNIVERSITY   (Australia)

^d TOYAMA PREFECTURAL UNIVERSITY   (Japan)

^e AALTO UNIVERSITY   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

GLUCURONOSYLTRANSFERASE; GLUCURONOSYLTRANSFERASE 1A1; GLUCURONOSYLTRANSFERASE 1A9; GLUCURONOSYLTRANSFERASE 2B7; RECOMBINANT ENZYME;

ANIMAL CELL; ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME KINETICS; FEMALE; GLUCURONIDATION; IMMUNODETECTION; INSECT CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ANIMALS; BACULOVIRIDAE; BLOTTING, WESTERN; CATALYTIC DOMAIN; ENZYME ACTIVATION; GLUCURONIDES; GLUCURONOSYLTRANSFERASE; HISTIDINE; HUMANS; KINETICS; MODELS, BIOLOGICAL; MUTATION; RECOMBINANT FUSION PROTEINS; SF9 CELLS; SPODOPTERA; SUBSTRATE SPECIFICITY; TRANSFECTION;

HEXAPODA;

EID: 84866467905     PISSN: 00909556     EISSN: 1521009X     Source Type: Journal    
DOI: 10.1124/dmd.112.046086     Document Type: Article

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