Investigation of coculture of human adipose-derived stem cells and mature adipocytes

Applied Biochemistry and Biotechnology

Volumn 167, Issue 8, 2012, Pages 2381-2387

  Song, Kedong ^a   Li, Wenfang ^a   Wang, Hong ^a   Wang, Hai ^b   Liu, Tianqing ^a   Ning, Ruiming ^a   Wang, Ling ^b  

^a DALIAN UNIVERSITY OF TECHNOLOGY   (China)

^b DALIAN MEDICAL UNIVERSITY   (China)

Author keywords

Adipocytes; Adipogenic differentiation; Adipose derived stem cells; Coculture; Oil Red O staining

Indexed keywords

ADIPOCYTES; ADIPOGENIC; ADIPOSE DERIVED STEM CELLS; COCULTURE; OIL RED O STAINING;

STEM CELLS;

SELF ASSEMBLY;

DYE; ENDOGLIN; HOE 33342; OIL RED O; TRYPAN BLUE; UNCLASSIFIED DRUG;

ADIPOCYTE; ADIPOSE DERIVED STEM CELL; ANTIGEN EXPRESSION; ARTICLE; CELL DIFFERENTIATION; CELL VIABILITY; COCULTURE; CONTROLLED STUDY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; HUMAN TISSUE; STAINING;

ADIPOCYTES; ANTIGENS, CD15; CELL DIFFERENTIATION; CELLS, CULTURED; COCULTURE TECHNIQUES; HUMANS; STEM CELLS;

EID: 84866378499     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-012-9764-y     Document Type: Article

References (20)

1. Duan, X., Duan, Q. Y., & Zhang, H. X. (2007). Application of stem cells in adipose tissue engineering. Journal of Clinical Rehabilitative Tissue Engineering Research, 11, 7433-7437.
2. Zuk, P. A., Zhu, M., Mizuno, H., Huang, J., Futrell, J. W., Katz, A. J., Benhaim, P., Lorenz, H. P., & Hedrick, M. H. (2001). Multilineage cells from human adipose tissue: Implications for cell-based therapies. Tissue Engineering, 7, 211-228.
3. Zuk, P. A., Zhu, M., Ashjian, P., De Ugarte, D. A., Huang, J. I., Mizuno, H., Alfonso, Z. C., Fraser, J. K., Benhaim, P., & Hedrick, M. H. (2002). Human adipose tissue is a source of multipotent stem cells. Molecular Biology of the Cell, 13(12), 4279-4295.
4. Schäffler, A., & Büchler, C. (2007). Concise review: Adipose tissue-derived stromal cells-basic and clinical implications for novel cell-based therapies. Stem Cells, 25, 818-827.
5. Lei, L., Liao, W. M., Sheng, P. Y., et al. (2003). Biological characteristics of human adipose-derived stem cells in vitro and effect of donor age on the proliferation. Science in China, 37, 163-170.
6. Yamashiro, H., Inamoto, T., Yagi, M., et al. (2003). Efficient proliferation and adipose differentiation of human adipose tissue-derived vascular stromal cells transfected with basic fibroblast growth factor gene. Tissue Engineering, 9, 881-892.
7. Wang, S. Q., & Xu, J. W. (2007). Methods of co-culture stem cells and differentiation. Implications for cell-based therapies. Tissue Engineering, 11, 2122-2125.
8. Maurin, A. C., Chavassieux, P. M., Frappart, L., et al. (2000). Influence of mature adipocytes on osteoblast proliferation in human primary cocultures. Bone, 26, 485-489.
9. Aoki, S., Toda, S., Sakemi, T., et al. (2003). Coculture of endothelial cells and mature adipocytes actively promotes immature preadipocyte development in vitro. Cell Structure and Function, 28, 55-60.
10. Wang, B., Han, J., Gao, Y., et al. (2007). The differentiation of rat adipose-derived stem cells into OEClike cells on collagen scaffolds by co-culturing with OECs. Neuroscience Letters, 421, 191-196.
11. Zhu, Y. X., Liu, T. Q., Song, K. D., et al. (2009). Adipose tissue-derived stem cells differentiated into cardiomyocytes in cardiac microenvironment. Progress in Biochemistry and Biophysics, 36, 624-632.
12. Jiang, L., Song, K., Jiang, B., Liu, T., Zhu, Y., Ma, X., & Cui, Z. (2008). Expansion of human adiposederived stem cells in suspension culture in spinner flasks. Tissue Engineering. Part A, 14(5), 734.
13. Zhu, Y., Liu, T., Song, K., Fan, X., Ma, X., & Cui, Z. (2009). Ex vivo expansion of adipose tissue-derived stem cells in spinner flasks. Biotechnology Journal, 4(8), 1198-1209.
14. Zhu, Y., Liu, T., Song, K., Ning, R., Ma, X., & Cui, Z. (2009). ADSCs differentiated into cardiomyocytes in cardiac microenvironment. Molecular and Cellular Biochemistry, 324(1-2), 117-129.
15. Zhu, Y., Liu, T., Song, K., Jiang, B., Ma, X., & Cui, Z. (2009). Collagen-chitosan polymer as a scaffold for the proliferation of human adipose tissue-derived stem cells. Journal of Materials Science. Materials in Medicine, 20(3), 799-808.
16. Zhu, Y., Liu, T., Ye, H., Song, K., Ma, X., & Cui, Z. (2010). Enhancement of adipose-derived stem cell differentiation in scaffolds with IGF-I gene impregnation under dynamic microenvironment. Stem Cells and Development, 19(10), 1547-1556.
17. Song, K., Qiao, M., Liu, T., Jiang, B., Macedo, H. M., Ma, X., & Cui, Z. (2010). Preparation, fabrication and biocompatibility of novel injectable temperature-sensitive chitosan/glycerophosphate/collagen hydrogels. Journal of Materials Science. Materials in Medicine, 21(10), 2835-2842.
18. Zhu, Y., Liu, T., Song, K., Fan, X., Ma, X., & Cui, Z. (2008). Adipose-derived stem cell: A better stem cell than BMSC. Cell Biochemistry and Function, 26, 664-675.
19. Rodbell, M. (1964). Metabolism of isolated fat cells: I. Effects of hormones on glucose metabolism and lipolysis. Biological Chemistry, 239, 375-380.
20. Zhang, H. H., Kumar, S., Barnett, A. H., & Eggo, M. C. (2000). Ceiling culture of mature human adipocytes: Use in studies of adipocyte functions. Endocrinology, 164, 119-128.