Accumulation of pre-let-7g and downregulation of mature let-7g with the depletion of EWS

Biochemical and Biophysical Research Communications

Volumn 426, Issue 1, 2012, Pages 89-93

  Sohn, Eun Jung ^a,b   Park, Junhong ^a   Kang, Soo Im ^a   Wu, Yun Ping ^a  

^a NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

^b Pusan National University School of Medicine   (South Korea)

Author keywords

Ewing sarcoma gen; Precursor microRNA; Primary microRNA

Indexed keywords

CYCLIN D1; DICER; PEPTIDES AND PROTEINS; PROTEIN LET 7G; RNA BINDING PROTEIN EWS; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ARTICLE; CANCER CELL CULTURE; CELL MATURATION; CONTROLLED STUDY; DOWN REGULATION; EWING SARCOMA; GENE INACTIVATION; GENE TARGETING; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEPLETION; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; UPREGULATION; WESTERN BLOTTING;

ANIMALS; BONE NEOPLASMS; CELL LINE, TUMOR; DOWN-REGULATION; HUMANS; MICE; MICE, KNOCKOUT; MICRORNAS; RIBONUCLEASE III; RNA PRECURSORS; RNA SPLICING; RNA-BINDING PROTEIN EWS; SARCOMA, EWING;

EID: 84866336448     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2012.08.041     Document Type: Article

References (32)

1. Bertolotti A., Lutz Y., Heard D.J., Chambon P., Tora L. hTAF(II)68, a novel RNA/ssDNA-binding protein with homology to the pro-oncoproteins TLS/FUS and EWS is associated with both TFIID and RNA polymerase II. The EMBO Journal 1996, 15:5022-5031.
2. Morohoshi F., Ootsuka Y., Arai K., Ichikawa H., Mitani S., Munakata N., Ohki M. Genomic structure of the human RBP56/hTAFII68 and FUS/TLS genes. Gene 1998, 221:191-198.
3. Bertolotti A., Melot T., Acker J., Vigneron M., Delattre O., Tora L. EWS, but not EWS-FLI-1, is associated with both TFIID and RNA polymerase II: interactions between two members of the TET family, EWS and hTAFII68, and subunits of TFIID and RNA polymerase II complexes. Molecular and Cellular Biology 1998, 18:1489-1497.
4. Knoop L.L., Baker S.J. The splicing factor U1C represses EWS/FLI-mediated transactivation. The Journal of Biological Chemistry 2000, 275:24865-24871.
5. Chansky H.A., Hu M., Hickstein D.D., Yang L. Oncogenic TLS/ERG and EWS/Fli-1 fusion proteins inhibit RNA splicing mediated by YB-1 protein. Cancer Research 2001, 61:3586-3590.
6. Paronetto M.P., Minana B., Valcarcel J. The Ewing sarcoma protein regulates DNA damage-induced alternative splicing. Molecular Cell 2011, 43:353-368.
7. Li H., Watford W., Li C., Parmelee A., Bryant M.A., Deng C., O'Shea J., Lee S.B. Ewing sarcoma gene EWS is essential for meiosis and B lymphocyte development. The Journal of Clinical Investigation 2007, 117:1314-1323.
8. Cho J., Shen H., Yu H., Li H., Cheng T., Lee S.B., Lee B.C. Ewing sarcoma gene Ews regulates hematopoietic stem cell senescence. Blood 2011, 117:1156-1166.
9. Gregory R.I., Yan K.P., Amuthan G., Chendrimada T., Doratotaj B., Cooch N., Shiekhattar R. The microprocessor complex mediates the genesis of microRNAs. Nature 2004, 432:235-240.
10. Han J., Lee Y., Yeom K.H., Kim Y.K., Jin H., Kim V.N. The Drosha-DGCR8 complex in primary microRNA processing. Genes & Development 2004, 18:3016-3027.
11. Hutvagner G., McLachlan J., Pasquinelli A.E., Balint E., Tuschl T., Zamore P.D. A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA. Science 2001, 293:834-838.
12. Ketting R.F., Fischer S.E., Bernstein E., Sijen T., Hannon G.J., Plasterk R.H., Interference Dicer functions in RNA Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans. Genes & Development 2001, 15:2654-2659.
13. Kim D.H., Behlke M.A., Rose S.D., Chang M.S., Choi S., Rossi J.J. Synthetic dsRNA Dicer substrates enhance RNAi potency and efficacy. Nature Biotechnology 2005, 23:222-226.
14. Sohn E.J., Li H., Reidy K., Beers L.F., Christensen B.L., Lee S.B. EWS/FLI1 oncogene activates caspase 3 transcription and triggers apoptosis in vivo. Cancer Research 2010, 70:1154-1163.
15. Kim J., Inoue K., Ishii J., Vanti W.B., Voronov S.V., Murchison E., Hannon G., Abeliovich A. A microRNA feedback circuit in midbrain dopamine neurons. Science 2007, 317:1220-1224.
16. Viswanathan S.R., Daley G.Q., Gregory R.I. Selective blockade of microRNA processing by Lin28. Science 2008, 320:97-100.
17. Fuchs B., Inwards C.Y., Janknecht R. Vascular endothelial growth factor expression is up-regulated by EWS-ETS oncoproteins and Sp1 and may represent an independent predictor of survival in Ewing's sarcoma. Clinical Cancer Research: An Official Journal of the American Association for Cancer Research 2004, 10:1344-1353.
18. Sanchez G., Bittencourt D., Laud K., Barbier J., Delattre O., Auboeuf D., Dutertre M. Alteration of cyclin D1 transcript elongation by a mutated transcription factor up-regulates the oncogenic D1b splice isoform in cancer. Proceedings of the National Academy of Sciences of the United States of America 2008, 105:6004-6009.
19. Fukuma M., Okita H., Hata J., Umezawa A. Upregulation of Id2, an oncogenic helix-loop-helix protein, is mediated by the chimeric EWS/ets protein in Ewing sarcoma. Oncogene 2003, 22:1-9.
20. Bailly R.A., Bosselut R., Zucman J., Cormier F., Delattre O., Roussel M., Thomas G., Ghysdael J. DNA-binding and transcriptional activation properties of the EWS-FLI-1 fusion protein resulting from the t(11;22) translocation in Ewing sarcoma. Molecular and Cellular Biology 1994, 14:3230-3241.
21. Lund E., Guttinger S., Calado A., Dahlberg J.E., Kutay U. Nuclear export of microRNA precursors. Science 2004, 303:95-98.
22. Bohnsack M.T., Czaplinski K., Gorlich D. Exportin 5 is a RanGTP-dependent dsRNA-binding protein that mediates nuclear export of pre-miRNAs. RNA 2004, 10:185-191.
23. Yi R., Qin Y., Macara I.G., Cullen B.R. Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs. Genes & Development 2003, 17:3011-3016.
24. Bennasser Y., Chable-Bessia C., Triboulet R., Gibbings D., Gwizdek C., Dargemont C., Kremer E.J., Voinnet O., Benkirane M. Competition for XPO5 binding between Dicer mRNA pre-miRNA and viral RNA regulates human Dicer levels. Nature Structural & Molecular Biology 2011, 18:323-327.
25. May W.A., Lessnick S.L., Braun B.S., Klemsz M., Lewis B.C., Lunsford L.B., Hromas R., Denny C.T. The Ewing's sarcoma EWS/FLI-1 fusion gene encodes a more potent transcriptional activator and is a more powerful transforming gene than FLI-1. Molecular and Cellular Biology 1993, 13:7393-7398.
26. Tsuchiya T., Sekine K., Hinohara S., Namiki T., Nobori T., Kaneko Y. Analysis of the p16INK4, p14ARF, p15, TP53, and MDM2 genes and their prognostic implications in osteosarcoma and Ewing sarcoma. Cancer Genetics and Cytogenetics 2000, 120:91-98.
27. Ban J., Jug G., Mestdagh P., Schwentner R., Kauer M., Aryee D.N., Schaefer K.L., Nakatani F., Scotlandi K., Reiter M., Strunk D., Speleman F., Vandesompele J., Kovar H. Hsa-mir-145 is the top EWS-FLI1-repressed microRNA involved in a positive feedback loop in Ewing's sarcoma. Oncogene 2011, 30:2173-2180.
28. De Vito C., Riggi N., Suva M.L., Janiszewska M., Horlbeck J., Baumer K., Provero P., Stamenkovic I. Let-7a is a direct EWS-FLI-1 target implicated in Ewing's sarcoma development. PLoS ONE 2011, 6:e23592.
29. Liang X.H., Crooke S.T. Depletion of key protein components of the RISC pathway impairs pre-ribosomal RNA processing. Nucleic Acids Research 2011, 39:4875-4889.
30. Bennasser Y., Chable-Bessia C., Triboulet R., Gibbings D., Gwizdek C., Dargemont C., Kremer E.J., Voinnet O., Benkirane M. Competition for XPO5 binding between Dicer mRNA, pre-miRNA and viral RNA regulates human Dicer levels. Nature Structural & Molecular Biology 2011, 18:323-327.
31. Lee B.J., Cansizoglu A.E., Suel K.E., Louis T.H., Zhang Z., Chook Y.M. Rules for nuclear localization sequence recognition by karyopherin beta 2. Cell 2006, 126:543-558.
32. Guttinger S., Muhlhausser P., Koller-Eichhorn R., Brennecke J., Kutay U. Transportin2 functions as importin and mediates nuclear import of HuR. Proceedings of the National Academy of Sciences of the United States of America 2004, 101:2918-2923.