Optimization and characterization of a novel FGF21 mutant

Yaoxue Xuebao

Volumn 47, Issue 7, 2012, Pages 897-903

  Ye, Xian Long ^a   Gao, Hua Shan ^a   Wang, Wen Fei ^a   Ren, Gui Ping ^a   Liu, Ming Yao ^a   He, Kun ^a   Zhang, Ya Kun ^a   Zhao, Jing Zhuang ^a   Yu, Dan ^a   Li, De Shan ^a  

^a NORTHEAST AGRICULTURAL UNIVERSITY   (China)

Author keywords

FGF21; Glucose uptake; Type 2 diabetes

Indexed keywords

ANTIDIABETIC AGENT; FIBROBLAST GROWTH FACTOR 21; HYBRID PROTEIN; SUMO PROTEIN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CELL STRAIN HEPG2; CONTROLLED STUDY; ESCHERICHIA COLI; GLUCOSE BLOOD LEVEL; GLUCOSE METABOLISM; GLUCOSE TRANSPORT; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; MOLECULAR CLONING; MOUSE; NONHUMAN; PROTEIN DOMAIN; PROTEIN EXPRESSION;

AMINO ACID SEQUENCE; ANIMALS; BLOOD GLUCOSE; DIABETES MELLITUS, EXPERIMENTAL; ENDOPEPTIDASES; ESCHERICHIA COLI; FIBROBLAST GROWTH FACTORS; GENETIC VECTORS; GLUCOSE; HEP G2 CELLS; HUMANS; HYPOGLYCEMIC AGENTS; MALE; MICE; MUTATION; PLASMIDS; RECOMBINANT FUSION PROTEINS; TRANSFORMATION, GENETIC;

EID: 84866089922     PISSN: 05134870     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (20)

1. Nishimura T, Nakatake Y, Konishi M, et al. Identification of a novel FGF, FGF21, preferentially expressed in the liver[J]. Biochim Biophys Acta, 2000, 1492: 203-206.
2. Zhang XQ, Albrahimi OA, Olsen SK, et al. Receptor specificity of the fibroblast growth factor family[J]. J Biol Chem, 2006, 281: 15694-15700.
3. Kharitonenkov A, Shiyanova TL, Koester A, et al. FGF21 as a novel metabolic regulator[J]. J Clin Invest, 2005, 115: 1627-1635.
4. Kharitonenkov A, Wroblewski VJ, Koester A, et al. The metabolic state of diabetic monkeys is regulated by fibroblast growth factor-221[J]. Endocrinology, 2007, 148: 774-781.
5. Liu MY, Wang WF, Yu YX, et al. FGF21 improves glucose uptake and glycogen synthesis of insulin-resistant liver cells[J]. Prog Biochem Biophys, 2009, 36: 1327-1333.
6. Jiang YY, Liu MY, Ren GP, et al. Cloning, expression and purification of mouse fibroblast growth factor-21 and its function in adipocyte glucose metabolism[J]. Prog Biochem Biophys, 2009, 36: 157-164.
7. Wente W, Efanov AM, Brenner M, et al. Fibroblast growth factor-21 improves pancreatic beta-cell function and survival by activation of extracellular signal-regulated kinase 1/2 and Akt signaling pathways[J]. Diabetes, 2006, 55: 2470-2478.
8. Wang HY, Xiao YC, Fu LJ, et al. High-level expression and purification of soluble recombinant FGF21 protein by SUMO fusion in Escherichia coli[J]. BMC Biotechnol, 2010, 10: 14.
9. Yao WB, Ren GP, Li DS, et al. Expression and pharmacological evaluation of fusion protein FGF21-L-Fc[J]. Acta Pharm Sin, 2011, 46: 787-792.
10. Cintia R, Amir A, Dan ST. Directed evolution of proteins for heterologous expression and stability[J]. Curr Opin Struct Biol, 2005, 15: 50-56.
11. Cheng J, Randall A, Baldi P. Prediction of protein stability changes for single-site mutations using support vector machines [J]. Proteins, 2006, 62: 1125-1132.
12. Eswar N, John B, Mirkovic N, et al. Tools for comparative protein structure modeling and analysis[J]. Nucleic Acids Res, 2003, 31: 3375-3380.
13. Gao HM, Wang WF, Zhang Q, et al. Establishment of a novel cell model targeted on FGF-21 receptor for screening antidiabetic drug candidates[J]. Acta Pharm Sin, 2011, 46: 904-909.
14. Mai K, Schwarz F, Bobbert T, et al. Relation between fibroblast growth factor-21, adiposity, metabolism and weight reduction[J]. Metabolism, 2011, 60: 306-311.
15. Melchior WR, Jaber LA. Metformin: an antihyperglycemic agent for treatment of type II diabetes[J]. Ann Pharmacother, 1996, 30: 158-164.
16. Ogawa Y, Kurosu H, Yamamoto M, et ai. BetaKlotho is required for metabolic activity of fibroblast growth factor 21[J]. Proc Natl Acad Sci USA, 2007, 104: 7432-7437.
17. Huang X, Yu C, Jin C, et al. Forced expression of hepatocyte-specific fibroblast growth factor-21 delays initiation of chemically-induced hepatocarcinogenesis[J]. Mol Carcinog, 2006, 42: 1215-1224.
18. Yeh BK, Igarashi M, Eliseenkova AV, et al. Structural basis by which alternative splicing confers specificity in fibroblast growth factor receptor[J]. Proc Natl Acad Sci USA, 2003, 100: 2266-2271.
19. Bellosta P, Iwahori A, Plotnikov AN, et al. Identification of receptor and heparin binding sites in fibroblast growth factor 4 by structure-based mutagenesis[J]. Mol Cell Biol, 2001, 21: 5946-5957.
20. Regina G, Andrew B, Omar A, et al. Molecular insights into the klotho-dependent, endocrine mode of action of fibroblast growth factor 19 subfamily members[J]. Mol Cell Biol, 2007, 27: 3417-3428.