Evaluation of EMLA Cream for Preventing Pain during Tattooing of Rabbits: Changes in Physiological, Behavioural and Facial Expression Responses

PLoS ONE

Volumn 7, Issue 9, 2012, Pages

  Keating, Stephanie C J ^a   Thomas, Aurelie A ^b   Flecknell, Paul A ^b   Leach, Matthew C ^b  

^a UNIVERSITY OF GUELPH   (Canada)

^b NEWCASTLE UNIVERSITY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CORTICOSTERONE; EMLA;

ANALGESIC ACTIVITY; ANIMAL BEHAVIOR; ANIMAL EXPERIMENT; ARTERIAL PRESSURE; ARTICLE; CONTROLLED STUDY; CORTICOSTERONE BLOOD LEVEL; DRUG EFFICACY; DRUG SCREENING; FACIAL EXPRESSION; FEMALE; HEART RATE; LOCAL ANESTHESIA; MALE; MEAN ARTERIAL PRESSURE; NONHUMAN; PAIN; PAIN ASSESSMENT; RABBIT; RATING SCALE; TATTOOING; TOPICAL ANESTHESIA; VOCALIZATION;

ANESTHETICS, LOCAL; ANIMALS; BEHAVIOR, ANIMAL; CROSS-OVER STUDIES; FACE; LIDOCAINE; OINTMENTS; PAIN; PRILOCAINE; RABBITS; TATTOOING;

ANIMALIA; ORYCTOLAGUS CUNICULUS;

EID: 84866077963     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0044437     Document Type: Article

References (32)

1. Mrozec M, Fischer R, Trendelenburg M, Zillmann U, (1995) Microchip implant system used for animal identification in laboratory rabbits, guineapigs, woodchucks and in amphibians. Lab Anim 29: 339-344.
2. Kasanan IHE, Voipio H-M, Leskinen H, Luodonpää M, Nevalainen TO, (2011) Comparison of ear tattoo, ear notching and microtattoo in rats undergoing cardiovascular telemetry. Lab Anim 45: 154-159.
3. Donovan J, Brown P (2006) Animal identification. Curr Protoc Immunol DOI: 10.1002/0471142735.im0105s73.
4. Brodbelt DC, Blissitt KJ, Hannond RA, Neath PJ, Young LE, et al. (2008) The risk of death: the confidential enquiry into perioperative small animal fatalities. Vet Anaesth Analg 35: 365-373.
5. American Rabbit Breeders Association (2012) Instructions to registrars. Available: http://www.arba.net/PDFs/instructions_to_registrars.pdf. Accessed 13 March 2012.
6. Flecknell PA, Liles JH, Williamson HA, (1990) The use of lignocaine-prilocaine local anaesthetic cream for pain-free venepuncture in laboratory animals. Lab Anim 24: 142-146.
7. Smith M, Gray BM, Ingram S, Jewkes DA, (1990) Double-blind comparison of topical lignocaine-prilocaine cream (EMLA) and lignocaine infiltration for arterial cannulation in adults. Br J Anaesth 65: 240-242.
8. Taddio A, Nulman I, Goldbach M, Ipp M, Koren G, (1994) Use of lidocaine-prilocaine cream for vaccination pain in infants. J Pediatr 124: 643-648.
9. Goodacre TEE, Sanders R, Watts DA, Stoker M, (1988) Split skin grafting using topical local anaesthesia (EMLA): a comparison with infiltrated anaesthesia. Br J Plast Surg 41: 533-538.
10. Shaikh FM, Naqvi SA, Grace PA, (2009) The influence of a eutectic mixture of lidocaine and prilocaine on minor surgical procedures: a randomized controlled double-blind trial. Dermatol Surg 35: 948-951.
11. Leach MC, Coulter CA, Richardson CA, Flecknell PA, (2011) Are We Looking in the Wrong Place? Implications for Behavioural-Based Pain Assessment in Rabbits (Oryctolagus cuniculi) and Beyond? PLoS One 6: e13347.
12. Stasiak KL, Maul D, French E, Hellyer PW, VandeWoude S, (2003) Species-specific assessment of pain in laboratory animals. Contemp Top Lab Anim Sci 42: 13-20.
13. Flecknell PA, (1994) Refinement of animal use-assessment and alleviation of pain and distress. Lab Anim 28: 222-231.
14. Sandküler J, (2009) Models and mechanisms of hyperalgesia and allodynia. J Physiol Rev 89: 707-758.
15. Martin TJ, Buechler NL, Kahn W, Crews JC, Eisenach JC, (2004) Effects of laparotomy on spontaneous exploratory activity and conditioned operant responding in the rat: a model for postoperative pain. Anesthesiology 101: 191-203.
16. Liles JH, Flecknell PA, (1994) A comparison of the effects of buprenorphine, carprofen and flunixin following laparotomy in rats. J Vet Pharm Therap 17: 284-290.
17. Leach MC, Allweiler S, Richardson C, Roughan JV, Narbe R, et al. (2009) Behavioural effects of ovariohysterectomy and oral administration of meloxicam in laboratory housed rabbits. Res Vet Sci 87: 336-347.
18. Leach MC, Klaus K, Miller AL, Scotto di Perrotolo M, Sotocinal SG, et al. (2012) The Assessment of Post-Vasectomy Pain in Mice Using Behaviour and the Mouse Grimace Scale. PLoS ONE 7(4): e35656 doi:10.1371/journal.pone.0035656.
19. Langford DJ, Bailey AL, Chanda ML, Clarke SE, Drummond TW, et al. (2010) Coding of facial expressions of pain in the laboratory mouse. Nat Methods 7: 447-449.
20. Sotocinal SG, Sorge RE, Zaloum A, Tuttle AH, Martin LJ, et al. (2011) The Rat Grimace Scale: A partially automated method for quantifying pain in the laboratory rat via facial expressions. Mol Pain DOI:10.1186/1744-8069-7-55.
21. Williams AC, (2002) Facial expression of pain: an evolutionary account. Behav Brain Sci 25: 439-455.
22. Roughan JV, Flecknell PA, (2004) Behaviour-based assessment of the duration of laparotomy-induced abdominal pain and the analgesic effects of carprofen and buprenorphine in rats. Behav Pharmacol 15: 461-472.
23. Roughan JV, Flecknell PA, (2001) Behavioural effects of laparotomy and analgesic effects of ketoprofen and carprofen in rats. Pain 90: 65-74.
24. Balcombe JP, Barnard ND, Sandusky C, (2004) Laboratory routines cause animal stress. Contemp Top Lab Anim Sci 43: 42-51.
25. National Research Council (2009) Recognition and alleviation of pain in laboratory animals. Washington DC: National Academies Press. 196 p.
26. Stasiak KL, Maul D, French E, Hellyer PW, Vandewoude Sue, (2003) Species-Specific assessment of pain in laboratory animals. J Am Ass Lab Ani Sci 42: 13-20.
27. Friedman PM, Mafong EA, Friedman ES, Geronemus RG, (2001) Topical anesthetics update: EMLA and beyond. Dermatol Surg 27: 1019-1026.
28. Holmes HS, (1994) Choosing a local anesthetic. Dermatol Clin 12: 817-823.
29. Rylander E, Sjoberg I, Lillieborg S, Stockman O, (1990) Local anesthesia of the genital mucosa with a lidocaine/prilocaine cream (EMLA) for laser treatment of condylomata acuminata: a placebo-controlled study. Obstet Gynecol 75: 302-306.
30. Wahlgren CF, Quiding H, (2000) Depth of cutaneous analgesia after application of a eutectic mixture of the local anesthetics lidocaine and prilocaine (EMLA cream). J Am Acad Dermatol 42: 584-588.
31. Hirvonen J, Rytting JH, Paronen P, Urtti A, (1991) Dodecyl N,N-dimethylamino acetate and azone enhance drug penetration across human, snake, and rabbit skin. Pharm Res 8: 933-937.
32. Bjerring P, Arendt-Nielsen L, (1990) Depth and duration of skin analgesia to needle insertion after topical application of EMLA cream. Br J Anaesth 64: 173-177.