Interference of engineered nanoparticles with in vitro toxicity assays

Archives of Toxicology

Volumn 86, Issue 7, 2012, Pages 1123-1136

  Kroll, Alexandra ^a,b   Pillukat, Mike Hendrik ^a   Hahn, Daniela ^a   Schnekenburger, Jürgen ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

^b SWISS FEDERAL INSTITUTE OF AQUATIC SCIENCE AND TECHNOLOGY   (Switzerland)

Author keywords

Cytokine adsorption; Cytotoxicity assays; Engineered nanoparticles; Interference

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; DICHLOROFLUORESCEIN; INTERLEUKIN 8; LACTATE DEHYDROGENASE; NANOPARTICLE; TITANIUM DIOXIDE; ZINC OXIDE;

ACCURACY; ARTICLE; CELL ASSAY; CELL DEATH; CELL FREE SYSTEM; CELL VIABILITY; CONCENTRATION (PARAMETERS); CYTOKINE PRODUCTION; CYTOTOXICITY TEST; DOWN REGULATION; ENZYME ACTIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; IN VITRO STUDY; NANOENGINEERING; NANOTOXICOLOGY; OXIDATIVE STRESS; PHYSICAL CHEMISTRY; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DEPLETION; TOXICITY TESTING;

ADSORPTION; CELL DEATH; CELL LINE; CYTOKINES; FLUORESCENT DYES; GERMANY; HUMANS; LUNG; MATERIALS TESTING; METAL NANOPARTICLES; NANOPARTICLES; NANOTECHNOLOGY; OXIDANTS; OXIDATIVE STRESS; PARTICLE SIZE; PHYSICOCHEMICAL PHENOMENA; QUARTZ; SOOT; TOXICITY TESTS; ZINC OXIDE;

EID: 84866019700     PISSN: 03405761     EISSN: 14320738     Source Type: Journal    
DOI: 10.1007/s00204-012-0837-z     Document Type: Article

References (47)

1. Belyanskaya L, Manser P, Spohn P, Bruinink A, Wick P (2007) The reliability and limits of the MTT reduction assay for carbon nanotubes-cell interaction. Carbon 45:2643-2648
2. Brown DM, Dickson C, Duncan P, Al-Attili F, Stone V (2010) Interaction between nanoparticles and cytokine proteins: impact on protein and particle functionality. Nanotechnology 21:215104
3. Carlsson H, Prachayasittikul V, Bulow L (1993) Zinc ions bound to chimeric His4/lactate dehydrogenase facilitate decarboxylation of oxaloacetate. Protein Eng 6:907-911
4. Casey A, Herzog E, Davoren M, Lyng FM, Byrne HJ, Chambers G (2007) Spectroscopic analysis confirms the interactions between single walled carbon nanotubes and various dyes commonly used to assess cytotoxicity. Carbon 45:1425-1432
5. Casey A, Herzog E, Lyng FM, Byrne HJ, Chambers G, Davoren M (2008) Single walled carbon nanotubes induce indirect cytotoxicity by medium depletion in A549 lung cells. Toxicol Lett 179:78-84
6. Cedervall T, Lynch I, Lindman S, Berggard T, Thulin E, Nilsson H, Dawson KA, Linse S (2007) Understanding the nanoparticleprotein corona using methods to quantify exchange rates and affinities of proteins for nanoparticles. Proc Nat Acad Sci USA 104:2050-2055
7. Cho SJ, Maysinger D, Jain M, Roder B, Hackbarth S, Winnik FM (2007) Long-term exposure to CdTe quantum dots causes functional impairments in live cells. Langmuir 23:1974-1980
8. Doak SH, Griffiths SM, Manshian B, Singh N, Williams PM, Brown AP, Jenkins GJ (2009) Confounding experimental considerations in nanogenotoxicology. Mutagenesis 24:285-293
9. Duffin R, Tran L, Brown D, Stone V, Donaldson K (2007) Proinflammogenic effects of low-toxicity and metal nanoparticles in vivo and in vitro: highlighting the role of particle surface area and surface reactivity. Inhalation Toxicol 19:849-856
10. Esch RK, Han L, Foarde KK, Ensor DS (2010) Endotoxin contamination of engineered nanomaterials. Nanotoxicology 4:73-83
11. Fisichella M, Dabboue H, Bhattacharyya S, Saboungi ML, Salvetat JP, Hevor T, Guerin M (2009) Mesoporous silica nanoparticles enhance MTT formazan exocytosis in HeLa cells and astrocytes. Toxicol In Vitro 23:697-703
12. Grassian VH, O'Shaughnessy PT, Adamcakova-Dodd A, Pettibone JM, Thorne PS (2007) Inhalation exposure study of titanium dioxide nanoparticles with a primary particle size of 2 to 5 nm. Environ Health Perspect 115:397-402
13. Guo L, Von Dem Bussche A, Buechner M, Yan A, Kane AB, Hurt RH (2008) Adsorption of essential micronutrients by carbon nanotubes and the implications for nanotoxicity testing. Small (Weinheim an der Bergstrasse, Germany) 4:721-727
14. Jakubowski W, Bartosz G (2000) 2,7-dichlorofluorescin oxidation and reactive oxygen species: what does it measure? Cell Biol Int 24:757-760
15. Johnston HJ, Hutchison GR, Christensen FM, Aschberger K, Stone V (2009) The biological mechanisms and physicochemical characteristics responsible for driving fullerene toxicity. Toxicol Sci 114:162-182
16. Kim H, Liu X, Kobayashi T, Kohyama T, Wen FQ, Romberger DJ, Conner H, Gilmour PS, Donaldson K, MacNee W, Rennard SI (2003) Ultrafine carbon black particles inhibit human lung fibroblast-mediated collagen gel contraction. Am J Respir Cell Mol Biol 28:111-121
17. Kocbach A, Totlandsdal AI, Lag M, Refsnes M, Schwarze PE (2008) Differential binding of cytokines to environmentally relevant particles: a possible source for misinterpretation of in vitro results? Toxicol Lett 176:131-137
18. Korzeniewski C, Callewaert DM (1983) An enzyme-release assay for natural cytotoxicity. J Immunol Methods 64:313-320
19. Kroll A, Pillukat MH, Hahn D, Schnekenburger J (2009) Current in vitro methods in nanoparticle risk assessment: limitations and challenges. Eur J Pharm Biopharm 72:370-377
20. Kroll A, Dierker C, Rommel C, Hahn D, Wohlleben W, Schulze- Isfort C, Gobbert C, Voetz M, Hardinghaus F, Schnekenburger J (2011) Cytotoxicity screening of 23 engineered nanomaterials using a test matrix of ten cell lines and three different assays. Part Fibre Toxicol 8:9
21. Landsiedel R, Ma-Hock L, Kroll A, Hahn D, Schnekenburger J, Wiench K, Wohlleben W (2010) Testing metal-oxide nanomaterials for human safety. Adv Mater 22:2601-2627
22. Lundqvist M, Stigler J, Elia G, Lynch I, Cedervall T, Dawson KA (2008) Nanoparticle size and surface properties determine the protein corona with possible implications for biological impacts. Proc Nat Acad Sci USA 105:14265-14270
23. Mahmoudi M, Simchi A, Imani M, Shokrgozar MA, Milani AS, Hafeli UO, Stroeve P (2010) A new approach for the in vitro identification of the cytotoxicity of superparamagnetic iron oxide nanoparticles. Colloids Surf 75:300-309
24. Monteiro-Riviere NA, Inman AO (2006) Challenges for assessing carbon nanomaterial toxicity to the skin. Carbon 44:1070-1078
25. Monteiro-Riviere NA, Inman AO, Zhang LW (2009) Limitations and relative utility of screening assays to assess engineered nanoparticle toxicity in a human cell line. Toxicol Appl Pharmacol 234:222-235
26. Nachlas MM, Margulies SI, Goldberg JD, Seligman AM (1960) The determination of lactic dehydrogenase with a tetrazolium salt. Anal Biochem 1:317-326
27. NanoCare Project (2009) NanoCare: health related aspects of nanomaterials. Final Scientific Report, Dechema e.V., Frankfurt a.M
28. Oberdorster G, Oberdorster E, Oberdorster J (2005) Nanotoxicology: an emerging discipline evolving from studies of ultrafine particles. Environ Health Perspect 113:823-839
29. Pan Z, Lee W, Slutsky L, Clark RA, Pernodet N, Rafailovich MH (2009) Adverse effects of titanium dioxide nanoparticles on human dermal fibroblasts and how to protect cells. Small 5:511-520
30. Pfaller T, Colognato R, Nelissen I, Favilli F, Casals E, Ooms D, Leppens H, Ponti J, Stritzinger R, Puntes V, Boraschi D, Duschl A, Oostingh GJ (2010) The suitability of different cellular in vitro immunotoxicity and genotoxicity methods for the analysis of nanoparticle-induced events. Nanotoxicology 4:52-72
31. Rabolli V, Thomassen LC, Princen C, Napierska D, Gonzalez L, Kirsch-Volders M, Hoet PH, Huaux F, Kirschhock CE, Martens JA, Lison D (2010) Influence of size, surface area and microporosity on the in vitro cytotoxic activity of amorphous silica nanoparticles in different cell types. Nanotoxicology 4:307-318
32. Rothen-Rutishauser B, Brown DM, Piallier-Boyles M, Kinloch IA, Windle AH, Gehr P, Stone V (2010) Relating the physicochemical characteristics and dispersion of multiwalled carbon nanotubes in different suspension media to their oxidative reactivity in vitro and inflammation in vivo. Nanotoxicology 4:331-342
33. Sabatini CA, Pereira RV, Gehlen MH (2007) Fluorescence modulation of acridine and coumarin dyes by silver nanoparticles. Journal of fluorescence 17:377-382
34. Sadik OA, Zhou AL, Kikandi S, Du N, Wang Q, Varner K (2009) Sensors as tools for quantitation, nanotoxicity and nanomonitoring assessment of engineered nanomaterials. J Environ Monit 11:1782-1800
35. Sayes CM, Wahi R, Kurian PA, Liu Y, West JL, Ausman KD, Warheit DB, Colvin VL (2006) Correlating nanoscale titania structure with toxicity: a cytotoxicity and inflammatory response study with human dermal fibroblasts and human lung epithelial cells. Toxicol Sci 92:174-185
36. Schulze C,Kroll A, Lehr CM, Schäfer UF, Becker K, Schnekenburger J, Schulze Isfort C, Landsiedel R, Wohleben W (2008) Not ready to use-overcoming pitfalls when dispersing nanoparticles in physiological media. Nanotoxicology 2:51-61
37. Seagrave J, Knall C, McDonald JD, Mauderly JL (2004) Diesel particulate material binds and concentrates a proinflammatory cytokine that causes neutrophil migration. Inhalation Toxicol 16(Suppl 1):93-98
38. Shaw SY, Westly EC, Pittet MJ, Subramanian A, Schreiber SL, Weissleder R (2008) Perturbational profiling of nanomaterial biologic activity. Proc Nat Acad Sci USA 105:7387-7392
39. Singh S, Nalwa HS (2007) Nanotechnology and health safety-toxicity and risk assessments of nanostructured materials on human health. J Nanosci Nanotechnol 7:3048-3070
40. Stearns RC, Paulauskis JD, Godleski JJ (2001) Endocytosis of ultrafine particles by A549 cells. Am J Respir Cell Mol Biol 24:108-115
41. Val S, Hussain S, Boland S, Hamel R, Baeza-Squiban A, Marano F (2009) Carbon black and titanium dioxide nanoparticles induce pro-inflammatory responses in bronchial epithelial cells: need for multiparametric evaluation due to adsorption artifacts. Inhalation Toxicol 21(Suppl 1):115-122
42. Veranth JM, Kaser EG, Veranth MM, Koch M, Yost GS (2007) Cytokine responses of human lung cells (BEAS-2B) treated with micron-sized and nanoparticles of metal oxides compared to soil dusts. Particle and fibre toxicology 4:2
43. Wang J, Chen C, Liu Y, Jiao F, Li W, Lao F, Li Y, Li B, Ge C, Zhou G, Gao Y, Zhao Y, Chai Z (2008) Potential neurological lesion after nasal instillation of TiO(2) nanoparticles in the anatase and rutile crystal phases. Toxicol Lett 183:72-80
44. Wells AJ, Smith WR (1941) The absorption spectrum of suspensions of carbon black. J Phys Chem 45:1055-1060
45. Wolf R, Wolf D, Morganti P, Ruocco V (2001) Sunscreens. Clin Dermatol 19:452-459
46. Worle-Knirsch JM, Pulskamp K, Krug HF (2006) Oops they did it again! Carbon nanotubes hoax scientists in viability assays. Nano Lett 6:1261-1268
47. 2 nanoparticles on the optical properties of resin composites. Dent Mater 25:1142-1147