Heterologous expression, purification and cofactor reconstitution of the reductive dehalogenase PceA from Dehalobacter restrictus

Protein Expression and Purification

Volumn 85, Issue 2, 2012, Pages 224-229

  Sjuts, Hanno ^a   Fisher, Karl ^a   Dunstan, Mark S ^a   Rigby, Stephen E ^a   Leys, David ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Cobalamin; EPR; Fe S cluster; Organohalide respiration; PceA; Trigger factor

Indexed keywords

BACTERIAL PROTEIN; CYANOCOBALAMIN; HYBRID PROTEIN; OXIDOREDUCTASE;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; COENZYME; ELECTRON SPIN RESONANCE; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; METABOLISM; MOLECULAR CLONING; PEPTOCOCCACEAE; POLYACRYLAMIDE GEL ELECTROPHORESIS; REPRODUCIBILITY;

BACTERIAL PROTEINS; CLONING, MOLECULAR; COENZYMES; ELECTRON SPIN RESONANCE SPECTROSCOPY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; OXIDOREDUCTASES; PEPTOCOCCACEAE; RECOMBINANT FUSION PROTEINS; REPRODUCIBILITY OF RESULTS; VITAMIN B 12;

EID: 84866006041     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2012.08.007     Document Type: Article

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