Detection of Viable Mycobacterium ulcerans in Clinical Samples by a Novel Combined 16S rRNA Reverse Transcriptase/IS2404 Real-Time qPCR Assay

PLoS Neglected Tropical Diseases

Volumn 6, Issue 8, 2012, Pages

  Beissner, Marcus ^a   Symank, Dominik ^a   Phillips, Richard Odame ^b   Amoako, Yaw Ampem ^b   Awua Boateng, Nana Yaa ^c   Sarfo, Fred Stephen ^b   Jansson, Moritz ^a   Huber, Kristina Lydia ^a   Herbinger, Karl Heinz ^a   Battke, Florian ^d   Löscher, Thomas ^a   Adjei, Ohene ^b   Bretzel, Gisela ^a  

^a LUDWIG MAXIMILIANS UNIVERSITY   (Germany)

^b KWAME NKRUMAH UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Ghana)

^c KUMASI CENTRE FOR COLLABORATIVE RESEARCH IN TROPICAL MEDICINE   (Ghana)

^d Dr Battke SCIENTIA GmbH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

RIFAMPICIN; RNA 16S; STREPTOMYCIN;

ARTICLE; BACTERIAL LOAD; BACTERIAL SURVIVAL; BACTERIAL VIABILITY; BURULI ULCER; CLINICAL ARTICLE; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; GENE; HUMAN; IS 2404 GENE; LIMIT OF DETECTION; MINIMUM INHIBITORY CONCENTRATION; MOLECULAR DIAGNOSIS; MYCOBACTERIUM ULCERANS; POLYMERASE CHAIN REACTION; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; SENSITIVITY AND SPECIFICITY; VALIDATION PROCESS;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; BACTERIOLOGICAL TECHNIQUES; BURULI ULCER; CHILD; DNA TRANSPOSABLE ELEMENTS; DNA, BACTERIAL; FEMALE; HUMANS; MALE; MIDDLE AGED; MOLECULAR DIAGNOSTIC TECHNIQUES; MYCOBACTERIUM ULCERANS; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY; YOUNG ADULT;

EID: 84865985002     PISSN: 19352727     EISSN: 19352735     Source Type: Journal    
DOI: 10.1371/journal.pntd.0001756     Document Type: Article

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