Critical phosphoprotein elements that regulate polymerase architecture and function in vesicular stomatitis virus

Proceedings of the National Academy of Sciences of the United States of America

Volumn 109, Issue 36, 2012, Pages 14628-14633

  Rahmeh, Amal A ^a   Morin, Benjamin ^a   Schenk, Andreas D ^a   Liang, Bo ^a   Heinrich, Bianca S ^a   Brusic, Vesna ^a   Walz, Thomas ^a,b   Whelan, Sean P J ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

^b HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

Large polymerase; Mononegavirales; Replication and transcription; Rhabdovirus

Indexed keywords

CATALYTIC PROTEIN; PHOSPHOPROTEIN; PROTEIN; RNA DIRECTED RNA POLYMERASE; UNCLASSIFIED DRUG;

ARTICLE; CARBOXY TERMINAL SEQUENCE; NONHUMAN; PRIORITY JOURNAL; RNA SYNTHESIS; RNA VIRUS; VESICULAR STOMATITIS VIRUS;

BLOTTING, WESTERN; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; MASS SPECTROMETRY; MICROSCOPY, ELECTRON; MODELS, BIOLOGICAL; NUCLEOCAPSID PROTEINS; PHOSPHOPROTEINS; PROTEIN CONFORMATION; RNA REPLICASE; VESICULOVIRUS; VIRAL PROTEINS; VIRAL STRUCTURAL PROTEINS; VIRUS REPLICATION;

MONONEGAVIRALES; RHABDOVIRIDAE; RNA VIRUSES; VESICULAR STOMATITIS VIRUS;

EID: 84865963708     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1209147109     Document Type: Article

References (30)

1. Emerson SU, Wagner RR (1972) Dissociation and reconstitution of the transcriptase and template activities of vesicular stomatitis B and T virions. J Virol 10:297-309.
2. Banerjee AK, Rhodes DP (1973) In vitro synthesis of RNA that contains polyadenylate by virion-associated RNA polymerase of vesicular stomatitis virus. Proc Natl Acad Sci USA 70:3566-3570.
3. Emerson SU, Wagner RR (1973) L protein requirement for in vitro RNA synthesis by vesicular stomatitis virus. J Virol 12:1325-1335.
4. Green TJ, Zhang X, Wertz GW, Luo M (2006) Structure of the vesicular stomatitis virus nucleoprotein-RNA complex. Science 313:357-360. (Pubitemid 44480961)
5. Albertini AA, et al. (2006) Crystal structure of the rabies virus nucleoprotein-RNA complex. Science 313:360-363. (Pubitemid 44480962)
6. Sleat DE, Banerjee AK (1993) Transcriptional activity and mutational analysis of recombinant vesicular stomatitis virus RNA polymerase. J Virol 67:1334-1339. (Pubitemid 23067639)
7. Ogino T, Banerjee AK (2007) Unconventional mechanism of mRNA capping by the RNA-dependent RNA polymerase of vesicular stomatitis virus. Mol Cell 25:85-97. (Pubitemid 46049056)
8. Li J, Fontaine-Rodriguez EC, Whelan SP (2005) Amino acid residues within conserved domain VI of the vesicular stomatitis virus large polymerase protein essential for mRNA cap methyltransferase activity. J Virol 79:13373-13384. (Pubitemid 41508151)
9. Barr JN, Whelan SP, Wertz GW (2002) Transcriptional control of the RNA-dependent RNA polymerase of vesicular stomatitis virus. Biochim Biophys Acta 1577:337-353. (Pubitemid 35223223)
10. Rahmeh AA, Li J, Kranzusch PJ, Whelan SP (2009) Ribose 2?-O methylation of the vesicular stomatitis virus mRNA cap precedes and facilitates subsequent guanine-N-7 methylation by the large polymerase protein. J Virol 83:11043-11050.
11. Emerson SU, Schubert M (1987) Location of the binding domains for the RNA polymerase L and the ribonucleocapsid template within different halves of the NS phosphoprotein of vesicular stomatitis virus. Proc Natl Acad Sci USA 84:5655-5659.
12. Mellon MG, Emerson SU (1978) Rebinding of transcriptase components (L and NS proteins) to the nucleocapsid template of vesicular stomatitis virus. J Virol 27: 560-567. (Pubitemid 9009095)
13. Li J, Rahmeh A, Morelli M, Whelan SP (2008) A conserved motif in region v of the large polymerase proteins of nonsegmented negative-sense RNA viruses that is essential for mRNA capping. J Virol 82:775-784.
14. Ding H, Green TJ, Lu S, Luo M (2006) Crystal structure of the oligomerization domain of the phosphoprotein of vesicular stomatitis virus. J Virol 80:2808-2814. (Pubitemid 43346375)
15. Leyrat C, et al. (2010) Structural disorder in proteins of the rhabdoviridae replication complex. Protein Pept Lett 17:979-987.
16. Howard M, Wertz G (1989) Vesicular stomatitis virus RNA replication: A role for the NS protein. J Gen Virol 70:2683-2694. (Pubitemid 19248815)
17. 0-P complex formation and encapsidation of viral genome RNA. J Virol 81:13478-13485. (Pubitemid 350247842)
18. Green TJ, Luo M (2009) Structure of the vesicular stomatitis virus nucleocapsid in complex with the nucleocapsid-binding domain of the small polymerase cofactor, P. Proc Natl Acad Sci USA 106:11713-11718.
19. Gerard FC, et al. (2007) Unphosphorylated rhabdoviridae phosphoproteins form elongated dimers in solution. Biochemistry 46:10328-10338. (Pubitemid 350067626)
20. Kolakofsky D, Le Mercier P, Iseni F, Garcin D (2004) Viral DNA polymerase scanning and the gymnastics of Sendai virus RNA synthesis. Virology 318:463-473. (Pubitemid 38224290)
21. 0-P complex. PLoS Pathog 7:e1002248.
22. Spadafora D, Canter DM, Jackson RL, Perrault J (1996) Constitutive phosphorylation of the vesicular stomatitis virus P protein modulates polymerase complex formation but is not essential for transcription or replication. J Virol 70:4538-4548. (Pubitemid 26187452)
23. Chattopadhyay D, Banerjee AK (1987) Phosphorylation within a specific domain of the phosphoprotein of vesicular stomatitis virus regulates transcription in vitro. Cell 49:407-414.
24. Rahmeh AA, et al. (2010) Molecular architecture of the vesicular stomatitis virus RNA polymerase. Proc Natl Acad Sci USA 107:20075-20080.
25. Morin B, Rahmeh AA, Whelan SP (2012) Mechanism of RNA synthesis initiation by the vesicular stomatitis virus polymerase. EMBO J 31:1320-1329.
26. Emerson SU, Yu Y (1975) Both NS and L proteins are required for in vitro RNA synthesis by vesicular stomatitis virus. J Virol 15:1348-1356.
27. Habchi J, Longhi S (2012) Structural disorder within paramyxovirus nucleoproteins and phosphoproteins. Mol Biosyst 8:69-81.
28. Green TJ, et al. (2011) Access to RNA encapsidated in the nucleocapsid of vesicular stomatitis virus. J Virol 85:2714-2722.
29. Das SC, Pattnaik AK (2004) Phosphorylation of vesicular stomatitis virus phosphoprotein P is indispensable for virus growth. J Virol 78:6420-6430. (Pubitemid 38715940)
30. Ohi M, Li Y, Cheng Y, Walz T (2004) Negative staining and image classification: Powerful tools in modern electron microscopy. Biol Proced Online 6:23-34. (Pubitemid 38597695)