Spicy SDS-PAGE Gels: Curcumin/Turmeric as an Environment-Friendly Protein Stain

Methods in Molecular Biology

Volumn 869, Issue , 2012, Pages 567-578

  Kurien, Biji T ^a   Dorri, Yaser ^a   Scofield, R Hal ^a  

^a UNIVERSITY OF OKLAHOMA HEALTH SCIENCES CENTER   (United States)

Author keywords

Coomassie Brilliant Blue; Curcumin; Environment friendly protein stain; SDS PAGE; Silver nitrate; Turmeric

Indexed keywords

ALCOHOL; ALKALI; BRILLIANT BLUE; CURCUMIN; FLUORESCENT DYE; SILVER; TURMERIC; WATER; BOVINE SERUM ALBUMIN; BUFFER; CELL EXTRACT; COLORING AGENT; COOMASSIE BRILLIANT BLUE; DIMETHYL SULFOXIDE; FUCHSINE; SILVER NITRATE; SODIUM HYDROXIDE; SOLVENT;

AQUEOUS SOLUTION; ARTICLE; CALORIMETRY; CONTROLLED STUDY; COST; CURCUMA LONGA; HEAT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN BINDING; RHIZOME; SENSITIVITY ANALYSIS; SOLUBILIZATION; STAINING; CHEMISTRY; CURCUMA; GREEN CHEMISTRY; HELA CELL; HUMAN; ISOLATION AND PURIFICATION; LIMIT OF DETECTION; METHODOLOGY; SOLUBILITY;

BUFFERS; CELL EXTRACTS; COLORING AGENTS; CURCUMA; CURCUMIN; DIMETHYL SULFOXIDE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ETHANOL; FLUORESCENT DYES; GREEN CHEMISTRY TECHNOLOGY; HELA CELLS; HUMANS; LIMIT OF DETECTION; ROSANILINE DYES; SERUM ALBUMIN, BOVINE; SILVER NITRATE; SODIUM HYDROXIDE; SOLUBILITY; SOLVENTS; STAINING AND LABELING; WATER;

EID: 84865830943     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-821-4_51     Document Type: Article

References (19)

1. D'souza A, Scofield RH (2009) Protein stains to detect antigen on membranes. Methods Mol Biol 536:433-440
2. Kurien BT, Scofield RH (1998) Heat mediated quick Coomassie blue protein staining and destaining of SDS-PAGE gels. Indian J Biochem Biophys 35:385-389
3. Steinberg TH (2009) Protein gel staining methods: an introduction and overview. Methods Enzymol 463:541-563, Review
4. Jin LT, Hwang SY, Yoo GS et al (2006) A mass spectrometry compatible silver Staining method for protein incorporating a new silver sensitizer in sodium dodecyl sulfate-polyacrylamide electrophoresis gels. Proteomics 6:2334-2337
5. Jin LT, Hwang SY, Yoo GS et al (2004) Sensitive silver staining of protein in sodium dodecyl sulfate-polyacrylamide gels using an azo dye, calconcarboxylic acid, as a silver-ion sensitizer. Electrophoresis 25:2494-2500
6. Møller HJ, Heinegård D, Poulsen JH (1993) Combined alcian blue and silver staining of subnanogram quantities of proteoglycans and glycosaminoglycans in sodium dodecyl sulfatepolyacrylamide gels. Anal Biochem 209:169-175
7. Pal JK, Godbole D, Sharma K (2004) Staining of proteins on SDS Polyacrylamide gels and on nitrocellulose membranes by Alta, a colour used as a cosmetic. J Biochem Biophys Methods 61:339-347
8. Lin CY, Wang V, Shui HA et al (2009) A comprehensive evaluation of imidazole-zinc reverse stain for current proteomic researches. Proteomics 9:696-709
9. Aggarwal BB, Kumar A, Aggarwal MS et al (2004) Curcumin derived from turmeric ( Curcuma longa ): a spice for all seasons. In: Bagchi D, Preuss HG (eds) Phytochemicals in cancer chemoprevention. CRC, Boca Raton, FL, pp 349-387
10. Aggarwal BB, Sung B (2009) Pharmacological basis for the role of curcumin in chronic diseases: an age-old spice with modern targets. Trends Pharmacol Sci 30:85-94
11. Bright JJ (2007) Curcumin and autoimmune disease. Adv Exp Med Biology 595:425-451, Review
12. Rowe DL, Ozbay T, O'Regan RM et al (2009) Modulation of the BRCA1 protein and induction of apoptosis in triple negative breast cancer cell lines by the polyphenolic. Breast Cancer 3:61-75
13. Anand P, Sundaram C, Jhurani S et al (2008) Curcumin and cancer: an "old-age" disease with an "age-old" solution. Cancer Lett 267:133-164, Review
14. Kurien BT, Singh A, Matsumoto H et al (2007) Improving the solubility and pharmacological efficacy of curcumin by heat treatment. Assay Drug Dev Technol 5:567-576
15. Kurien BT, Scofield RH (2009) Curry spice curcumin and prostate cancer. Mol Nutr Food Res 53:939-940
16. Kurien BT (2009) Comment on Curcumin attenuates acrylamide-induced cytotoxicity and genotoxicity in HepG2 cells by ROS scavenging. J Agric Food Chem 57:5644-5646
17. Kurien BT, Scofield RH (2009) Bubbling hookah smoke through heat-solubilized curcumin/turmeric and incorporation of the curry spice as an additive orfilter in cigarettes to minimize tobacco smoke-related toxicants. Med Hypotheses 73:462-463
18. Kurien BT, D'Souza A, Scofield RH (2010) Heat-solubilized curry spice curcumin inhibits antibody-antigen interaction in in vitro studies: A possible therapy to alleviate autoimmune disorders. Mol Nutr Food Res 54:1202-1209
19. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685