Protein quantification methods to determine protein concentration prior to electrophoresis

Methods in Molecular Biology

Volumn 869, Issue , 2012, Pages 29-35

  Goldring, J P Dean ^a  

^a UNIVERSITY OF KWAZULU NATAL   (South Africa)

Author keywords

Absorbance; Bicinchoninic acid; Bradford; Coomassie; Lowry; Protein staining

Indexed keywords

ARTICLE; CONCENTRATION (PARAMETERS); ENZYME ACTIVITY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ISOLATION; QUANTITATIVE ASSAY; CHEMISTRY; ISOLATION AND PURIFICATION; METHODOLOGY; REVIEW; STAINING; ULTRAVIOLET SPECTROPHOTOMETRY;

ANAZOLENE SODIUM; BICINCHONINIC ACID; COLORING AGENT; COPPER; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; FUCHSINE; PROTEIN; QUINOLINE DERIVATIVE;

COLORING AGENTS; COPPER; INDICATORS AND REAGENTS; PROTEINS; QUINOLINES; ROSANILINE DYES; SPECTROPHOTOMETRY, ULTRAVIOLET; STAINING AND LABELING;

EID: 84865828867     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-821-4_3     Document Type: Article

References (7)

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4. Bradford MM (1976) Rapid and sensitive method for quantitation of microgram quantities of protein utilizing principle of protein-dye binding. Anal Biochem 72: 248-254
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7. Groves WE, Davis FC, Sells BH (1968) Spectrophotometric determination of microgram quantities of protein without nucleic acid interference. Anal Biochem 22: 195-210