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Volumn 869, Issue , 2012, Pages 111-118

Protein electrophoresis in agarose gels for separating high molecular weight proteins

Author keywords

DATD; Large proteins; SeaKem Gold agarose; Titin

Indexed keywords

AGAROSE; CONNECTIN; MYOSIN HEAVY CHAIN; NEBULIN; ARTHROPOD PROTEIN; BUFFER; DODECYL SULFATE SODIUM; MUSCLE PROTEIN; SURFACTANT;

EID: 84865823914     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-821-4_10     Document Type: Article
Times cited : (11)

References (14)
  • 1
    • 0014949207 scopus 로고
    • Cleavage of structural proteins during the assembly of the head of bacteriophage T4
    • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
    • (1970) Nature , vol.227 , pp. 680-685
    • Laemmli, U.K.1
  • 2
    • 0014690791 scopus 로고
    • The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
    • Weber K, Osborn M (1969) The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem 244:4406-4412
    • (1969) J Biol Chem , vol.244 , pp. 4406-4412
    • Weber, K.1    Osborn, M.2
  • 3
    • 0027511216 scopus 로고
    • Gel electrophoresis of giant proteins: Solubilization and silver-staining of titin and nebulin from single musclefiber segments
    • Granzier H, Wang K (1993) Gel electrophoresis of giant proteins: solubilization and silver-staining of titin and nebulin from single musclefiber segments. Electrophoresis 14:56-64
    • (1993) Electrophoresis , vol.14 , pp. 56-64
    • Granzier, H.1    Wang, K.2
  • 4
    • 0028855384 scopus 로고
    • Detection of giant myofibrillar proteins connectin and nebulin by electrophoresis in 2% polyacrylamide slab gels strengthened with agarose
    • Tatsumi R, Hattori A (1995) Detection of giant myofibrillar proteins connectin and nebulin by electrophoresis in 2% polyacrylamide slab gels strengthened with agarose. Anal Biochem 224: 28-31
    • (1995) Anal Biochem , vol.224 , pp. 28-31
    • Tatsumi, R.1    Hattori, A.2
  • 5
    • 0033962880 scopus 로고    scopus 로고
    • Differential expression of cardiac titin isoforms and modulation of cellular stiffness
    • Cazorla O, Freiburg A, Helmes M et al (2000) Differential expression of cardiac titin isoforms and modulation of cellular stiffness. Circ Res 86:59-67
    • (2000) Circ Res , vol.86 , pp. 59-67
    • Cazorla, O.1    Freiburg, A.2    Helmes, M.3
  • 6
    • 0038219368 scopus 로고    scopus 로고
    • Vertical agarose gel electrophoresis and electroblotting of high-molecular-weight proteins
    • Warren CM, Krzesinski PR, Greaser ML (2003) Vertical agarose gel electrophoresis and electroblotting of high-molecular-weight proteins. Electrophoresis 24:1695-1702
    • (2003) Electrophoresis , vol.24 , pp. 1695-1702
    • Warren, C.M.1    Krzesinski, P.R.2    Greaser, M.L.3
  • 7
    • 33845499231 scopus 로고    scopus 로고
    • Characterization of a core binding site for ADAMTS-13 in the A2 domain of von Willebrand factor
    • Wu JJ, Fujikawa K, McMullen BA et al (2006) Characterization of a core binding site for ADAMTS-13 in the A2 domain of von Willebrand factor. Proc Natl Acad Sci USA 103:18470-18474
    • (2006) Proc Natl Acad Sci USA , vol.103 , pp. 18470-18474
    • Wu, J.J.1    Fujikawa, K.2    McMullen, B.A.3
  • 8
    • 77449110676 scopus 로고    scopus 로고
    • Analysis of von Willebrand factor multimers by simultaneous high-and lowresolution vertical SDS-agarose gel electrophoresis and Cy5-labeled antibody high-sensitivity fluorescence detection
    • Ott HW, Griesmacher A, Schnapka-Koepf M et al (2010) Analysis of von Willebrand factor multimers by simultaneous high-and lowresolution vertical SDS-agarose gel electrophoresis and Cy5-labeled antibody high-sensitivity fluorescence detection. Am J Clin Pathol 133:322-330
    • (2010) Am J Clin Pathol , vol.133 , pp. 322-330
    • Ott, H.W.1    Griesmacher, A.2    Schnapka-Koepf, M.3
  • 9
    • 25644434918 scopus 로고    scopus 로고
    • Purification of neuronal inclusions of patients with Huntington's disease reveals a broad range of N-terminal fragments of expanded huntingtin and insoluble polymers
    • Hoffner G, Island ML, Djian P (2005) Purification of neuronal inclusions of patients with Huntington's disease reveals a broad range of N-terminal fragments of expanded huntingtin and insoluble polymers. J Neurochem 95:125-136
    • (2005) J Neurochem , vol.95 , pp. 125-136
    • Hoffner, G.1    Island, M.L.2    Djian, P.3
  • 10
    • 34047133301 scopus 로고    scopus 로고
    • Disease proteomics of high-molecular-mass proteins by twodimensional gel electrophoresis with agarose gels in thefirst dimension (agarose 2-DE)
    • Oh-Ishi M, Maeda T (2007) Disease proteomics of high-molecular-mass proteins by twodimensional gel electrophoresis with agarose gels in thefirst dimension (agarose 2-DE). J Chromatogr B Analyt Technol Biomed Life Sci 849:211-222
    • (2007) J Chromatogr B Analyt Technol Biomed Life Sci , vol.849 , pp. 211-222
    • Oh-Ishi, M.1    Maeda, T.2
  • 11
    • 0021112289 scopus 로고
    • Quantitative determination of myosin and actin in rabbit skeletal muscle
    • Yates LD, Greaser ML (1983) Quantitative determination of myosin and actin in rabbit skeletal muscle. J Mol Biol 168:123-141
    • (1983) J Mol Biol , vol.168 , pp. 123-141
    • Yates, L.D.1    Greaser, M.L.2
  • 12
    • 0024402489 scopus 로고
    • Factors affecting polyacrylamide gel electrophoresis and electroblotting of high-molecularweight myofibrillar proteins
    • Fritz JD, Swartz DR, Greaser ML (1989) Factors affecting polyacrylamide gel electrophoresis and electroblotting of high-molecularweight myofibrillar proteins. Anal Biochem 180:205-210
    • (1989) Anal Biochem , vol.180 , pp. 205-210
    • Fritz, J.D.1    Swartz, D.R.2    Greaser, M.L.3
  • 13
    • 0021274472 scopus 로고
    • Quantitation of protein and DNA in silver-stained agarose gels
    • Peats S (1984) Quantitation of protein and DNA in silver-stained agarose gels. Anal Biochem 140:178-182
    • (1984) Anal Biochem , vol.140 , pp. 178-182
    • Peats, S.1
  • 14
    • 0032535467 scopus 로고    scopus 로고
    • Modification of cysteine residues by alkylation. A tool in peptide mapping and protein identification
    • Sechi S, Chait BT (1998) Modification of cysteine residues by alkylation. A tool in peptide mapping and protein identification. Anal Chem 70:5150-5158
    • (1998) Anal Chem , vol.70 , pp. 5150-5158
    • Sechi, S.1    Chait, B.T.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.