Real-time polymerase chain reaction testing for Clostridium difficile reduces isolation time and improves patient management in a small community hospital

American Journal of Infection Control

Volumn 40, Issue 7, 2012, Pages 663-666

  Catanzaro, Mary ^a   Cirone, Justin ^a  

^a Pocono Medical Center   (United States)

Author keywords

Accurate testing; Health care associated infection; Isolation reduction; Nosocomial infection

Indexed keywords

CLOSTRIDIUM DIFFICILE TOXIN A; CLOSTRIDIUM DIFFICILE TOXIN B; METRONIDAZOLE; VANCOMYCIN;

ANTIBIOTIC THERAPY; ARTICLE; BACTERIUM DETECTION; BACTERIUM ISOLATION; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM DIFFICILE INFECTION; COMMUNITY HOSPITAL; ENZYME IMMUNOASSAY; HOSPITAL INFECTION; HUMAN; INFECTION CONTROL; INFECTION RATE; MAJOR CLINICAL STUDY; PATIENT CARE; REAL TIME POLYMERASE CHAIN REACTION; RETROSPECTIVE STUDY;

AGED; AGED, 80 AND OVER; ANTI-BACTERIAL AGENTS; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM INFECTIONS; DRUG UTILIZATION; FEMALE; HOSPITALS, COMMUNITY; HUMANS; MALE; METRONIDAZOLE; MOLECULAR DIAGNOSTIC TECHNIQUES; PATIENT ISOLATION; REAL-TIME POLYMERASE CHAIN REACTION; RETROSPECTIVE STUDIES; TIME FACTORS; TREATMENT OUTCOME; VANCOMYCIN;

EID: 84865784824     PISSN: 01966553     EISSN: 15273296     Source Type: Journal    
DOI: 10.1016/j.ajic.2011.09.005     Document Type: Article

References (16)

1. E.R. Dubberke, and A.I. Wertheimer Review of current literature on the economic burden of Clostridium difficile infection Infect Control Hosp Epidemiol 30 2009 57 66
2. S.M. Poutanen, and A.E. Simor Clostridium difficile-associated diarrhea in adults Can Med Assoc J 171 2004 51 58
3. M.D. Redelings, F. Sorvillo, and L. Mascola Increase in Clostridium difficile-related mortality rates, United States, 1999-2004 Emerg Infect Dis 13 2007 1417 1419
4. W.R. Jarvis, J. Schlosser, A.A. Jarvis, and R.Y. Chinn National point prevalence of Clostridium difficile in US healthcare facility inpatients, 2008 Am J Infect Control 37 2009 263 270
5. S.H. Cohen, D.N. Gerding, S. Johnson, C.P. Kelly, V.P. Loo, and L.C. McDonald Clinical practice guidelines for Clostridium difficile infection in adults: 2010 update by the Society for Healthcare Epidemiology of America (SHEA) and the Infectious Diseases Society of America (IDSA) Infect Control Hosp Epidemiol 31 2010 431 455
6. L.V. McFarland, G.W. Elmer, and C.M. Surawicz Breaking the cycle: treatment strategies for 163 cases of recurrent Clostridium difficile disease Am J Gastroenterol 97 2002 1769 1775
7. L. Petersen, R.U. Manson, S. Paule, D. Hacek, A. Robicsek, and R.B. Thomson Detection of toxigenic Clostridium difficile in stool samples by real-time polymerase chain reaction for the diagnosis of C. difficile-associated diarrhea Clin Infect Dis 45 2007 1152 1160
8. P.D. Stamper, R. Alcabasa, D. Aird, W. Babiker, J. Wehrlin, and I. Ikpeama Comparison of a commercial real-time PCR assay for tcdB detection to a cell culture cytotoxicity assay and toxigenic culture for direct detection of toxin-producing Clostridium difficile in clinical sample J Clin Microbiol 47 2009 373 378
9. G. Terhes, E. Urban, J. Soki, E. Nacsa, and E. Nagy Comparison of rapid molecular method, the BD GeneOhm Cdiff assay, to the most frequently used laboratory tests for detection of toxin-producing Clostridium difficile in diarrheal feces J Clin Microbiol 47 2009 3478 3481
10. L.C. McDonald, B. Coignard, E. Dubberke, X. Song, T. Horan, and P. Kutty Recommendations for surveillance of Clostridium difficile-associated disease Infect Control Hosp Epidemiol 28 2007 140 145
11. A. Larson, A.M. Fung, and F.C. Fang Evaluation of tcdB real-time PCR in a three-step diagnostic algorithm for detection of toxigenic Clostridium difficile J Clin Microbiol 48 2010 124 130
12. S.M. Novak-Weekley, E.M. Marlowe, J.M. Miller, J. Cumpio, J.H. Nomura, and P.H. Vance Clostridium difficile testing in the clinical laboratory by use of multiple testing algorithms J Clin Microbiol 48 2010 889 893
13. K. Eastwood, P. Else, A. Charlett, and M. Wilcox Comparison if nine commercially available Clostridium difficile toxin detection assays, a real-time PCR assay for C. difficile tcdB, and a glutamate dehydrogenase assay to cytotoxin testing and cytotoxigenic culture methods J Clin Microbiol 47 2009 3211 3217
14. B. Currie Real time PCR testing for CDI improves outcomes and reduces costs Med Lab Observer 41 2009 18
15. C. Abad, A. Fearday, and N. Safdar Adverse effects of isolation in hospitalized patients: a systemic review J Hosp Infect 76 2010 97 102
16. D.J. Morgan, D.J. Diekema, K. Sepkowitz, and E.N. Perencevich Adverse outcomes associated with contact precautions: a review in the literature Am J Infect Control 37 2009 85 93