Enterococcus faecalis produces abundant extracellular structures containing DNA in the absence of cell lysis during early biofilm formation

mBio

Volumn 3, Issue 4, 2012, Pages

  Barnes, Aaron M T ^a   Ballering, Katie S ^a   Leibman, Rachel S ^a,b   Wells, Carol L ^a   Dunnya, Gary M ^a  

^a UNIVERSITY OF MINNESOTA MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; DEOXYRIBONUCLEASE; EXTRACELLULAR DNA; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL CELL; BIOFILM; CELL LABELING; CELL SECRETION; CELL VIABILITY; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CYTOLYSIS; DNA DETERMINATION; DNA STRUCTURE; ENTEROCOCCUS FAECALIS; EXTRACELLULAR MATRIX; NONHUMAN; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; STRUCTURE ANALYSIS; BACTERIAL SECRETION SYSTEM; BACTERIOLYSIS; EXTRACELLULAR SPACE; GENETICS; METABOLISM; PHYSIOLOGY; ULTRASTRUCTURE;

BACTERIA (MICROORGANISMS); ENTEROCOCCUS FAECALIS; METAZOA; POSIBACTERIA;

BACTERIAL SECRETION SYSTEMS; BACTERIOLYSIS; BIOFILMS; DNA, BACTERIAL; ENTEROCOCCUS FAECALIS; EXTRACELLULAR SPACE; MICROSCOPY, ELECTRON, SCANNING;

EID: 84865776527     PISSN: 21612129     EISSN: 21507511     Source Type: Journal    
DOI: 10.1128/mBio.00193-12     Document Type: Article

References (25)

1. Flemming HC, Wingender J. 2010. The biofilm matrix. Nat. Rev. Microbiol. 8:623-633.
2. Lewis K. 2008. Multidrug tolerance of biofilms and persister cells. Curr. Top. Microbiol. Immunol. 322:107-131.
3. Whitchurch CB, Tolker-Nielsen T, Ragas PC, Mattick JS. 2002. Extracellular DNA required for bacterial biofilm formation. Science 295:1487.
4. Thomas VC, et al. 2009. A fratricidal mechanism is responsible for eDNA release and contributes to biofilm development of Enterococcus faecalis. Mol. Microbiol. 72:1022-1036.
5. Vilain S, Pretorius JM, Theron J, Brözel VS. 2009. DNA as an adhesin: Bacillus cereus requires extracellularDNAto form biofilms. Appl. Environ. Microbiol. 75:2861-2868.
6. Kreth J, Vu H, Zhang Y, Herzberg MC. 2009. Characterization of hydrogen peroxide-induced DNA release by Streptococcus sanguinis and Streptococcus gordonii. J. Bacteriol. 191:6281-6291.
7. Guiton PS, et al. 2009. Contribution of autolysin and Sortase A during Enterococcus faecalis DNA-dependent biofilm development. Infect. Immun. 77:3626-3638.
8. Wimpenny J, Manz W, Szewzyk U. 2000. Heterogeneity in biofilms. FEMS Microbiol. Rev. 24:661-671.
9. Spormann AM. 2008. Physiology of microbes in biofilms. Curr. Top. Microbiol. Immunol. 322:17-36.
10. Stewart PS, Franklin MJ. 2008. Physiological heterogeneity in biofilms. Nat. Rev. Microbiol. 6:199-210.
11. An D, Parsek MR. 2007. The promise and peril of transcriptional profiling in biofilm communities. Curr. Opin. Microbiol. 10:292-296.
12. Ballering KS. 2010. Identification and characterization of novel genetic determinants of biofilm formation in Enterococcus faecalis. Ph.D. thesis. University of Minnesota, Minneapolis, MN.
13. Dunny GM. 2007. The peptide pheromone-inducible conjugation system of Enterococcus faecalis plasmid pCF10: cell-cell signalling, gene transfer, complexity and evolution. Philos. Trans. R. Soc. Lond. B Biol. Sci. 362: 1185-1193.
14. Hamilton HL, Domínguez NM, Schwartz KJ, Hackett KT, Dillard JP. 2005. Neisseria gonorrhoeae secretes chromosomal DNA via a novel type IV secretion system. Mol. Microbiol. 55:1704-1721.
15. Rosch JW, Caparon MG. 2004. A microdomain for protein secretion in Gram-positive bacteria. Science 304:1513-1515.
16. Rosch JW, Caparon MG. 2005. The ExPortal: an organelle dedicated to the biogenesis of secreted proteins in Streptococcus pyogenes. Mol. Microbiol. 58:959-968.
17. Bourgogne A, et al. 2008. Large scale variation in Enterococcus faecalis illustrated by the genome analysis of strain OG1RF. Genome Biol. 9:R110.
18. Goodman SD, et al. 2011. Biofilms can be dispersed by focusing the immune system on a common family of bacterial nucleoid-associated proteins. Mucosal Immunol. 4:625-637.
19. Dunny GM, Brown BL, Clewell DB. 1978. Induced cell aggregation and mating in Streptococcus faecalis: evidence for a bacterial sex pheromone. Proc. Natl. Acad. Sci. U. S. A. 75:3479-3483.
20. Sahm DF, et al. 1989. In vitro susceptibility studies of vancomycinresistant Enterococcus faecalis. Antimicrob. Agents Chemother. 33: 1588-1591.
21. Kristich CJ, et al. 2008. Development and use of an efficient system for random mariner transposon mutagenesis to identify novel genetic determinants of biofilm formation in the core Enterococcus faecalis genome. Appl. Environ. Microbiol. 74:3377-3386.
22. Erlandsen SL, Kristich CJ, Dunny GM, Wells CL. 2004. High-resolution visualization of the microbial glycocalyx with low-voltage scanning electron microscopy: dependence on cationic dyes. J. Histochem. Cytochem. 52:1427-1435.
23. Erlandsen SL, Frethem C, Autrata R. 1990, Workshop on highresolution immunocytochemistry of cell surfaces using field emission SEM. J. Histochem. Cytochem. 38:1779-1780.
24. Shapiro HM. 2008. Flow cytometry of bacterial membrane potential and permeability. Methods Mol. Med. 142:175-186.
25. Wu J, Xi C. 2009. Evaluation of different methods for extracting extracellular DNA from the biofilm matrix. Appl. Environ. Microbiol. 75: 5390-5395.