RNAse mapping and quantitation of RNA isoforms

Methods in Molecular Biology

Volumn 883, Issue , 2012, Pages 121-129

  Venkatesh, Lakshminarayan K ^a   Fasina, Olufemi ^a   Pintel, David J ^a  

^a University of Missouri   (United States)

Author keywords

Denaturing polyacrylamide gel electrophoresis; High specific activity RNA probe; In vitro transcription; Ribonuclease protection assays; RNA hybridization; RNAse A and T1 digestion; SP6 or T7 RNA polymerase

Indexed keywords

MESSENGER RNA; RIBONUCLEASE; RNA; PANCREATIC RIBONUCLEASE; PHOSPHORUS; RIBONUCLEASE T1; RNA ISOFORM;

ARTICLE; BACTERIOPHAGE; BACTERIOPHAGE SP6; BACTERIOPHAGE T7; CYTOPLASM; ENZYME ACTIVITY; ENZYME ANALYSIS; GENETIC TRANSCRIPTION; MAMMAL CELL; NONHUMAN; OPEN READING FRAME; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; RNA PROBE; ANIMAL; AUTORADIOGRAPHY; CELL CULTURE; CHEMISTRY; DNA DENATURATION; GENETICS; HUMAN; METHODOLOGY; NUCLEIC ACID HYBRIDIZATION; RNA CLEAVAGE; RNA PROCESSING; SEQUENCE ANALYSIS; STAINING;

ANIMALS; AUTORADIOGRAPHY; CELLS, CULTURED; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; NUCLEIC ACID DENATURATION; NUCLEIC ACID HYBRIDIZATION; PHOSPHORUS RADIOISOTOPES; RIBONUCLEASE T1; RIBONUCLEASE, PANCREATIC; RIBONUCLEASES; RNA CLEAVAGE; RNA ISOFORMS; RNA PROBES; RNA PROCESSING, POST-TRANSCRIPTIONAL; RNA, MESSENGER; SEQUENCE ANALYSIS, RNA; STAINING AND LABELING; TRANSCRIPTION, GENETIC;

EID: 84865685217     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-839-9_9     Document Type: Article

References (7)

1. Gilman M (1997) Ribonuclease protection assay. In: Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds) Current protocols in molecular biology. Wiley Interscience, New York, NY, p 4.7.1
2. Sambrook J, Fritsch EF, Maniatis T (1989) Mapping of RNA with ribonuclease and radiolabeled RNA probes. In: Sambrook J, Fritsch EF, Maniatis T (eds) Molecular cloning: a laboratory manual, vol 2. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, p 7.71
3. Zinn K, DiMaio D, Maniatis T (1983) Identi fi cation of two distinct regulatory regions adjacent to the human b-interferon gene. Cell 34:865-879
4. Melton DA, Krieg PA, Rebagliati MR, Maniatis T, Zinn K, Green MR (1984) Ef fi cient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucl Acids Res 12:7035-7056
5. Qiu J, Pintel D (2008) Processing of adenoassociated virus RNA. Front Biosci 13:3101-3115
6. Qiu J, Yoto Y, Tullis G, Pintel DJ (2006) Parvovirus RNA processing strategies. In: Kerr JR, Cotmore SF, Bloom ME, Linden RM, Parrish CR (eds) Parvoviruses, Hodder Arnold, London, UK, pp 253-274
7. Zhao Q, Schoborg RV, Pintel DJ (1994) Alternative splicing of pre-mRNAs encoding the non-structural proteins of minute virus of mice is facilitated by sequences within the downstream intron. J Virol 60:2849-2859