RNA in situ hybridization in arabidopsis

Methods in Molecular Biology

Volumn 883, Issue , 2012, Pages 75-86

  Wu, Miin Feng ^a   Wagner, Doris ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

Alkaline phosphatase; DIG; FAA; In situ hybridization; NBT BCIP; Nonradioactive; Paraplast; Sections

Indexed keywords

ANTIBODY; PLANT RNA; ALCOHOL; DIGOXIGENIN; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; FIXATIVE; FORMALDEHYDE; RNA;

ARABIDOPSIS; ARTICLE; CROSS HYBRIDIZATION; IN SITU HYBRIDIZATION; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; RNA PROBE; RNA TRANSCRIPTION; CHEMISTRY; DESICCATION; GENETIC TRANSCRIPTION; GENETICS; HISTOLOGY; METHODOLOGY; STAINING;

ARABIDOPSIS; DESICCATION; DIGOXIGENIN; ETHANOL; FIXATIVES; FORMALDEHYDE; IN SITU HYBRIDIZATION; INDICATORS AND REAGENTS; RNA; RNA PROBES; RNA, PLANT; STAINING AND LABELING; TISSUE EMBEDDING; TRANSCRIPTION, GENETIC;

EID: 84865680202     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-839-9_5     Document Type: Article

References (6)

1. Pastore J et al (2011) LATE MERISTEM IDENTITY2 acts together with LEAFY to activate APETALA1 Development 138(15): 3189-3198
2. Long JA, Barton MK (1998) The development of apical embryonic pattern in Arabidopsis Development 125(16):3027-3035
3. Weigel D, Glazebrook J (2002) Arabidopsis : a laboratory manual. Cold Spring Harbor Laboratory, NewYork
4. Moench TR et al (1985) Effi ciency of in situ hybridization as a function of probe size and fi xation technique. J Virol Methods 11(2):119-130
5. Hayashi S et al (1978) Acetylation of chromosome squashes of Drosophila melanogaster decreases the background in autoradiographs from hybridization with [125I]-labeled RNA. J Histochem Cytochem 26(8):677-679
6. Blázquez M et al (2006) How fl oral meristems are built. Plant Mol Biol 60(6):855-870