Panbacterial real-time PCR to evaluate bacterial burden in chronic wounds treated with Cutimed™ Sorbact™

European Journal of Clinical Microbiology and Infectious Diseases

Volumn 31, Issue 7, 2012, Pages 1523-1529

  Gentili, V ^a   Gianesini, S ^a   Balboni, P G ^a   Menegatti, E ^a   Rotola, A ^a   Zuolo, M ^a   Caselli, E ^a   Zamboni, P ^a   Di Luca, D ^a  

^a UNIVERSITY OF FERRARA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

RNA 16S;

ARTICLE; BACTERIAL COUNT; BACTERIAL LOAD; BACTERIUM CULTURE; BACTEROIDES; CHRONIC WOUND; CLINICAL ARTICLE; CONTROLLED STUDY; CORRELATION ANALYSIS; DEBRIDEMENT; DOUBLE BLIND PROCEDURE; FUSOBACTERIUM; HUMAN; HYDROPHOBICITY; MEMBRANE BINDING; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; PUNCH BIOPSY; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; STAPHYLOCOCCUS AUREUS; TREATMENT OUTCOME; WOUND CARE; WOUND DRESSING; WOUND HEALING; BACTERIUM; CLASSIFICATION; EVALUATION; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; MIXED INFECTION; PILOT STUDY; WOUND INFECTION;

BACTERIA; BACTERIOLOGICAL TECHNIQUES; COINFECTION; DOUBLE-BLIND METHOD; HUMANS; PILOT PROJECTS; REAL-TIME POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; TREATMENT OUTCOME; WOUND INFECTION;

EID: 84865582371     PISSN: 09349723     EISSN: 14354373     Source Type: Journal    
DOI: 10.1007/s10096-011-1473-x     Document Type: Article

References (26)

1. Gjodsbol K, Christensen JJ, Karlsmark T, Jorgensen B, Klein BM, Krogfelt KA (2006) Multiple bacterial species reside in chronic wounds: A longitudinal study. Int Wound J 3:225-231
2. Frank DN, Wysocki A, Specht-Glick DD, Rooney A, Feldman RA, St. Amand AL, Pace NR, Trent JD (2009) Microbial diversity in chronic open wounds. Wound Repair Regen 17:163-172
3. Brook I, Frazier EH (1998) Aerobic and anaerobic microbiology of chronic venous ulcers. Int J Dermatol 37:426-428
4. Howell-Jones RS, Wilson MJ, Hill KE, Howard AJ, Price PE, Thomas DW (2005) A review of the microbiology, antibiotic usage and resistance in chronic skin wounds. J Antimicrob Chemother 55:143-149
5. Bowler PG, Duerden BI, Armstrong DG (2001) Wound microbiology and associated approaches to wound management. Clin Microbiol Rev 14:244-269
6. Bowler PG, Davies BJ (1999) The microbiology of infected and noninfected leg ulcers. Int J Dermatol 38:573-578
7. Madsen SM, Westh H, Danielsen L, Rosdahl VT (1996) Bacterial colonisation and healing of venous leg ulcers. APMIS 104:895-899
8. Wolcott RD, Dowd SE (2008) A rapid molecular method for characterising bacterial bioburden in chronic wounds. J Wound Care 17:513-516
9. Thomsen TR, Aasholm MS, Rudkjøbing VB, Saunders AM, Bjarnsholt T, Givskov M, Kirketerp-Møller K, Nielsen PH (2010) The bacteriology of chronic venous leg ulcer examined by culture-independent molecular methods. Wound Repair Regen 18:38-49
10. Martin JM, Zenilman JM, Lazarus GS (2010) Molecular microbiology: New dimensions for cutaneous biology and wound healing. J Invest Dermatol 130:38-48
11. Hill KE, Davies CE, Wilson MJ, Stephens P, Harding KG, Thomas DW (2003) Molecular analysis of the microflora in chronic venous leg ulceration. J Med Microbiol 52:365-369
12. Fang H, Hedin G (2003) Rapid screening and identification of methicillin-resistant Staphylococcus aureus from clinical samples by selective-broth and real-time PCR assay. J Clin Microbiol 41:2894-2899
13. Boutaga K, van Winkelhoff AJ, Vandenbroucke-Grauls CMJE, Savelkoul PHM (2005) Periodontal pathogens: A quantitative comparison of anaerobic culture and real-time PCR. FEMS Immunol Med Microbiol 45:191-199
14. Wilson KH, Blitchington RB, Greene RC (1990) Amplification of bacterial 16S ribosomal DNA with polymerase chain reaction. J Clin Microbiol 28:1942-1946
15. Davies CE, Hill KE, Wilson MJ, Stephens P, Hill CM, Harding KG, Thomas DW (2004) Use of 16S ribosomal DNA PCR and denaturing gradient gel electrophoresis for analysis of the microfloras of healing and nonhealing chronic venous leg ulcers. J Clin Microbiol 42:3549-3557
16. Doyle RJ, Rosenberg M (eds) (1990) Microbial cell surface hydrophobicity. ASM, Washington DC
17. Ljungh A, Wadström T (1995) Growth conditions influence expression of cell surface hydrophobicity of staphylococci and other wound infection pathogens. Microbiol Immunol 39:753-757
18. Ljungh A, Yanagisawa N, Wadström T (2006) Using the principle of hydrophobic interaction to bind and remove wound bacteria. J Wound Care 15:175-180
19. O'Meara S, Cullum N, Majid M, Sheldon T (2000) Systematic reviews of wound care management: (3) antimicrobial agents for chronic wounds; (4) diabetic foot ulceration. Health Technol Assess 4:1-237
20. Wright JB, Lam K, Burrell RE (1998) Wound management in an era of increasing bacterial antibiotic resistance: A role for topical silver treatment. Am J Infect Control 26:572-577
21. Yang S, Lin S, Kelen GD, Quinn TC, Dick JD, Gaydos CA, Rothman RE (2002) Quantitative multiprobe PCR assay for simultaneous detection and identification to species level of bacterial pathogens. J Clin Microbiol 40:3449-3454
22. Moore K, Huddleston E, StaceyMC, Harding KG (2007) Venous leg ulcer -the search for a prognostic indicator. IntWound J 4:163-172
23. Donovan S (1998) Wound infection and wound swabbing. Prof Nurse 13:757-759
24. Rantakokko-Jalava K, Nikkari S, Jalava J, Eerola E, Skurnik M, Meurman O, Ruuskanen O, Alanen A, Kotilainen E, Toivanen P, Kotilainen P (2000) Direct amplification of rRNA genes in diagnosis of bacterial infections. J Clin Microbiol 38:32-39
25. Fenollar F, Roux V, Stein A, Drancourt M, Raoult D (2006) Analysis of 525 samples to determine the usefulness of PCR amplification and sequencing of the 16S rRNA gene for diagnosis of bone and joint infections. J Clin Microbiol 44:1018-1028
26. Dowd SE, Delton Hanson J, Rees E, Wolcott RD, Zischau AM, Sun Y, White J, Smith DM, Kennedy J, Jones CE (2011) Survey of fungi and yeast in polymicrobial infections in chronic wounds. J Wound Care 20:40-47