Bursting the bubble on bacterial biofilms: A flow cell methodology

Biofouling

Volumn 28, Issue 8, 2012, Pages 835-842

  Crusz, Shanika A ^a   Popat, Roman ^a   Rybtke, Morten Theil ^b   Cámara, Miguel ^a   Givskov, Michael ^b,c   Tolker Nielsen, Tim ^b   Diggle, Stephen P ^a   Williams, Paul ^a  

^a UNIVERSITY OF NOTTINGHAM   (United Kingdom)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

^c NANYANG TECHNOLOGICAL UNIVERSITY   (Singapore)

Author keywords

biofilm; bubble trap; flow cell; method; protocol; Pseudomonas aeruginosa

Indexed keywords

BACTERIUM; BIOFILM; HYDRODYNAMICS; METHODOLOGY; RESEARCH WORK;

ARTICLE; BIOFILM; CULTURE TECHNIQUE; INSTRUMENTATION; PHYSIOLOGY; PSEUDOMONAS AERUGINOSA;

BIOFILMS; CELL CULTURE TECHNIQUES; PSEUDOMONAS AERUGINOSA;

BACTERIA (MICROORGANISMS); PSEUDOMONAS AERUGINOSA;

EID: 84865553273     PISSN: 08927014     EISSN: 10292454     Source Type: Journal    
DOI: 10.1080/08927014.2012.716044     Document Type: Article

References (11)

1. Coenye, T and Nelis, HJ. 2010. In vitro and in vivo model systems to study microbial biofilm formation. J Microbiol Meth, 83: 89-105.
2. Costerton, JW, Stewart, PS and Greenberg, EP. 1999. Bacterial biofilms: a common cause of persistent infections. Science, 284: 1318-1322.
3. Costerton, JW, Lewandowski, Z, Caldwell, DE, Korber, DR and Lappin-Scott, HM. 1995. Microbial biofilms. Annu Rev Microbiol, 49: 711-745.
4. Hall-Stoodley, L, Costerton, JW and Stoodley, P. 2004. Bacterial biofilms: from the natural environment to infectious diseases. Nat Rev Microbiol, 2: 95-108.
5. Heydorn, A, Nielsen, AT, Hentzer, M, Sternberg, C, Givskov, M, Ersbøll, BK and Molin, S. 2000. Quantification of biofilm structures by the novel computer program COMSTAT. Microbiology, 146: 2395-2407.
6. Merritt, JH, Kadouri, DE and O'Toole, GA. 2005. Growing and analyzing static biofilms. Curr Protoc Microbiol: Unit 1B.1,
7. Mueller, LN, de Brouwer, JF, Almeida, JS, Stal, LJ and Xavier, JB. 2006. Analysis of a marine phototrophic biofilm by confocal laser scanning microscopy using the new image quantification software PHLIP. BMC Ecol, 6: 1
8. Pamp, SJ, Sternberg, C and Tolker-Nielsen, T. 2009. Insight into the microbial multicellular lifestyle via flow-cell technology and confocal microscopy. Cytometry, 75: 90-103.
9. Peeters, E, Nelis, H and Coenye, T. 2008. Comparison of multiple methods for quantification of microbial biofilms grown in microtiter plates. J Microbiol Meth, 72: 157-165.
10. Tolker-Nielsen, T and Sternberg, C. 2011. Growing and analyzing biofilms in flow chambers. Curr Protoc Microbiol: Unit 1B.2,
11. Wolfaardt, GM, Lawrence, JR, Robarts, RD, Caldwell, SJ and Caldwell, DE. 1994. Multicellular organization in a degradative biofilm community. Appl Environ Microbiol, 60: 434-446.