Recombinant production, isotope labeling and purification of ENOD40B: A plant peptide hormone

Protein and Peptide Letters

Volumn 19, Issue 8, 2012, Pages 808-811

  Chae, Young Kee ^a,c   Tonneli, Marco ^b   Markley, John L ^b,c  

^a Sejong University   (South Korea)

^b National Magnetic Resonance Facility at Madison   (United States)

^c UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

MS; NMR; Nodule development; Peptide hormone; Ubiquitin fusion

Indexed keywords

EARLY NODULIN 40B; HYBRID PROTEIN; HYDROLASE; NITROGEN 15; NODULIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; YEAST UBIQUITIN HYDROLASE;

ARTICLE; CONTROLLED STUDY; ION PAIR CHROMATOGRAPHY; ISOTOPE LABELING; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PLASMID; PROTEIN CLEAVAGE; PROTEIN DENATURATION; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN IMMOBILIZATION; PROTEIN ISOLATION; PROTEIN PURIFICATION; PROTEIN SECRETION; PROTEIN SYNTHESIS; REVERSED PHASE LIQUID CHROMATOGRAPHY;

CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, PLANT; ISOTOPE LABELING; NITROGEN ISOTOPES; PEPTIDE HORMONES; PLANTS; RECOMBINANT FUSION PROTEINS; RNA, LONG UNTRANSLATED; UBIQUITIN;

EID: 84865380871     PISSN: 09298665     EISSN: None     Source Type: Journal    
DOI: 10.2174/092986612801619624     Document Type: Article

References (12)

1. Crespi, M.D.; Jurkevitch, E.; Poiret, M.; d'Aubenton-Carafa, Y.; Petrovics, G.; Kondorosi, E.; Kondorosi, A. enod40, a gene expressed during nodule organogenesis, codes for a nontranslatable RNA involved in plant growth. EMBO J., 1994, 13, 5099-5112. (Pubitemid 24341091)
2. Kouchi, H.; Hata, S. Isolation and Characterization of Novel Nodulin cDNAs Representing Genes Expressed at Early Stages of Soybean Nodule Development. Mol. Gen. Genet., 1993, 238, 106-119. (Pubitemid 23130640)
3. Kouchi, H.; Takane, K.; So, R.B.; Ladha, J.K.; Reddy, P.M. Rice ENOD40: isolation and expression analysis in rice and transgenic soybean root nodules. Plant J., 1999, 18, 121-129.
4. Charon, C.; Sousa, C.; Crespi, M.; Kondorosi, A. Alteration of enod40 expression modifies Medicago truncatula root nodule development induced by Sinorhizobium meliloti. Plant Cell, 1999, 11, 1953-1965.
5. Rohrig, H.; Schmidt, J.; Miklashevichs, E.; Schell, J.; John, M. Soybean ENOD40 encodes two peptides that bind to sucrose synthase. Proc. Natl. Acad. Sci. USA, 2002, 99, 1915-1920. (Pubitemid 34168989)
6. Rohrig, H.; John, M.; Schmidt, J. Modification of soybean sucrose synthase by S-thiolation with ENOD40 peptide A. Biochem. Bioph. Res. Co., 2004, 325, 864-870. (Pubitemid 39487952)
7. Thummler, F.; Verma, D.P.S. NODULIN-100 OF Soybeans Is The Subunit Of Sucrose Synthase Regulated By The Availability Of Free Heme In Nodules. J. Biol. Chem., 1987, 262, 14730-14736.
8. Gordon, A.J.; Minchin, F.R.; James, C.L.; Komina, O. Sucrose synthase in legume nodules is essential for nitrogen fixation. Plant Physiol., 1999, 120, 867-877. (Pubitemid 29384517)
9. Moon, W.J.; Hwang, D.K.; Park, E.J.; Kim, Y.M.; Chae, Y.K. Recombinant expression, isotope labeling, refolding, and purification of an antimicrobial peptide, piscidin. Protein Expr. Purif., 2007, 51, 141-146. (Pubitemid 44821873)
10. Xu, X.; Jin, F.; Yu, X.; Ren, S.; Hu, J.; Zhang, W. High-level expression of the recombinant hybrid peptide cecropinA(1-8)-magainin2(1-12) with an ubiquitin fusion partner in Escherichia coli. Protein Expr. Purif., 2007, 55, 175-182. (Pubitemid 47260742)
11. Delaglio, F.; Grzesiek, S.; Vuister, G.W.; Zhu, G.; Pfeifer, J.; Bax, A. NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J. Biomol. NMR, 1995, 6, 277-293.
12. Moon, W.J.; Hwang, D.K.; Park, E.J.; Kim, Y.M.; Chae, Y.K. Recombinant expression, isotope labeling, refolding, and purification of an antimicrobial peptide, piscidin. Protein Expr. Purif., 2007, 51, 141-146. (Pubitemid 44821873)