The OASIS® HLB μElution plate as a one-step platform for manual high-throughput in-gel digestion of proteins and peptide desalting

Proteomics

Volumn 12, Issue 15-16, 2012, Pages 2487-2492

  Franz, Thomas ^a   Li, Xinping ^a  

^a MAX PLANCK INSTITUTE FOR BIOLOGY OF AGEING   (Germany)

Author keywords

High throughput in gel digest; In gel digest and desalting platform; Microplate in gel digestion and desalting; OASIS HLB Elution plate; Technology

Indexed keywords

BACTERIAL PROTEIN;

ARTICLE; ELECTROSPRAY MASS SPECTROMETRY; ELUTION; INTERMETHOD COMPARISON; LIQUID CHROMATOGRAPHY; NONHUMAN; PRESSURE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PROCESSING; TWO DIMENSIONAL DIFFERENCE GEL ELECTROPHORESIS;

ANIMALS; DROSOPHILA MELANOGASTER; DROSOPHILA PROTEINS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; HIGH-THROUGHPUT SCREENING ASSAYS; PEPTIDES; PROTEOLYSIS; PROTEOMICS; SODIUM CHLORIDE;

EID: 84865354143     PISSN: 16159853     EISSN: 16159861     Source Type: Journal    
DOI: 10.1002/pmic.201100354     Document Type: Article

References (9)

1. Eckerskorn, C., Lottspeich, F., Internal amino acid sequence-analysis of proteins separated by gel-electrophoresis after tryptic digestion in polyacrylamide matrix. Chromatograhia 1989, 28, 92-94.
2. Rosenfeld, J., Capdevielle, J., Guillemot, J. C., Ferrara, P., In-Gel digestion of proteins for internal sequence analysis after one- or two-dimensional gel electrophoresis. Anal. Biochem. 1992, 203, 173-179.
3. Ashman, K., Houthaeve, T., Clayton, J., Wilm, M., et al., The application of robotics and mass spectrometry to the characterisation of the Drosophila melanogaster indirect flight muscle proteome. Lett. Pept. Sci. 1997, 4, 57-65.
4. Houthaeve, T., Gausepohl, H., Ashman, K., Nillson, T. et al., Automated Protein Preparation Techniques Using a Digest Robot. J. Protein Chem. 1997, 16, 343-348.
5. Dedeo, A., Chernokalskaya, E., Kopaciewicz, W., Clark, P. et al., Integrated system for identifying low abundant proteins from gels. Millipore Technical Publications, Lit No: PS1023EN00, 2003.
6. Nebrich, G., Herrmann, M., Sagi, D., Klose, J. P., High MS-compatibility of silver nitrate-stained protein spots from 2-DE gels using ZipPlates and AnchorChips for successful protein identification. Electrophoresis 2007, 28, 1607-1614.
7. Görg, A., Weiss, W., Dunn, M. J., Current two-dimensional electrophoresis technology for proteomics. Proteomics 2004, 4, 3665-3685.
8. Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227, 680-685.
9. Neuhoff, V., Stamm, R., Eibl, H., Clear background and highly sensitive protein staining with Coomassie Blue dyes in polyacrylamide gels - a systematic analysis. Electrophoresis 1985, 6, 427-448.