Caprylic acid precipitation method for impurity reduction: An alternative to conventional chromatography for monoclonal antibody purification

Biotechnology and Bioengineering

Volumn 109, Issue 10, 2012, Pages 2589-2598

  Brodsky, Yan ^a   Zhang, Cheng ^a,b   Yigzaw, Yinges ^a   Vedantham, Ganesh ^a  

^a AMGEN INC   (United States)

^b GENZYME CORPORATION   (United States)

Author keywords

Caprylic acid; Impurity precipitation; MAb process development; Purification

Indexed keywords

ALTERNATIVE METHODS; CAPRYLIC ACID; FLOW-THROUGH CHROMATOGRAPHIES; HIGH MOLECULAR WEIGHT; HOST CELL PROTEIN; MINIMAL COST; MONOCLONAL ANTIBODY PURIFICATION; OPERATIONAL PARAMETERS; POLISHING STEPS; PRIMARY FACTORS; PROCESS DEVELOPMENT; PROCESS FLOWS; PROCESS INTENSIFICATION; PROCESS TANKS; PRODUCTION FACILITY; PROTEIN A; PURIFICATION METHOD; REDUCTION METHOD; REDUCTION STUDIES; VOLUME CONSTRAINT;

AGGREGATES; AIR POLLUTION; CELL CULTURE; CHROMATOGRAPHY; LAKES; MOLECULAR WEIGHT; MONOCLONAL ANTIBODIES; POLISHING; PRECIPITATION (CHEMICAL); PRECIPITATION (METEOROLOGY); PROTEINS; PURIFICATION; VIRUSES;

SATURATED FATTY ACIDS;

MONOCLONAL ANTIBODY; OCTANOIC ACID;

ARTICLE; CHROMATOGRAPHY; HOST CELL; MOLECULAR WEIGHT; NONHUMAN; PH; PRECIPITATION; PROTEIN AGGREGATION; PURIFICATION; RETROVIRUS;

ANTIBODIES, MONOCLONAL; BIOTECHNOLOGY; CAPRYLATES; CHEMICAL PRECIPITATION; CHROMATOGRAPHY, AFFINITY; RETROVIRIDAE;

POLYGALA INCARNATA;

EID: 84865349291     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24539     Document Type: Article

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