Toxinotyping of Clostridium perfringens isolates by ELISA and PCR from lambs suspected of enterotoxemia

Turkish Journal of Veterinary and Animal Sciences

Volumn 36, Issue 4, 2012, Pages 409-415

  Hadimli, Hasan Hüseyin ^a   Erganiş, Osman ^a   Sayin, Zafer ^a   Aras, Zeki ^a  

^a SELCUK UNIVERSITY   (Turkey)

Author keywords

Clostridium perfringens; ELISA; Enterotoxemia; Lamb; PCR

Indexed keywords

EID: 84865330525     PISSN: 13000128     EISSN: 13036181     Source Type: Journal    
DOI: 10.3906/vet-1008-437     Document Type: Article

References (28)

1. Uzal, F.A.: Diagnosis of Clostridium perfringens intestinal infections in sheep and goats. Anaerobe, 1996; 10: 135-143.
2. Songer, J.G.: Clostridial enteric disease of domestic animals. Clin. Microbiol., 1996; 9: 216-234.
3. Petit, L., Gibert, M., Popoff , M.R.: Clostridium perfringens: toxinotype and genotype. Trends Microbiol., 1999; 7: 104-110.
4. Uzal, F.A., Songer, J.G.: Diagnosis of Clostridium perfringens intestinal infections in sheep and goats. J. Vet. Diagn. Invest., 2008; 20: 253-265.
5. Yamagashi, T., Sugitani, K., Tanishima, K., Nakamura, S.: Polymerase chain reaction test for differentiation of fi ve toxin types of Clostridium perfringens. Microbiol. Immun., 1997; 41: 295-299.
6. Greco, G., Madio, A., Buonavoglia, D., Totaro, M., Corrente, M., Martella, V., Buonavoglia, C.: Clostridium perfringens toxin-types in lambs and kids affected with gastroenteric pathologies in Italy. Vet. J., 2005; 170: 346-350.
7. McDonel, J.L.: Toxins of Clostridium perfringens type A, B, C, D, and E. In: Dorner, F., Drews, H. Eds., Pharmacology of Bacterial Toxins. Pergamon Press, Oxford. 1986.
8. El Idrissi, A.H., Ward, G.E.: Evaluation of enzyme-linked immunosorbent assay for diagnosis of Clostridium perfringens enterotoxemias. Vet. Microbiol., 1992; 31: 389-396.
9. Hunter, S.E.C., Braun, J.E., Oyston, P.C.F., Sakuvai, J., Titball, R.W.: Molecular genetic analysis of β-toxin of Clostridium perfringens reveals sequence homology with α-toxin, γ-toxin, and leukocidin of Staphylococcus aureus. Infect. Immun., 1993; 61: 3958-3965.
10. Gkiourtzidis, K., Frey, J., Bourtzi-Hatzopoulou, E., Iliadis, N., Sarris, K.: PCR detection and prevalence of α-, β-, β2-, ε-, ι- and enterotoxin genes in Clostridium perfringens isolated from lambs with clostridial dysentery. Vet. Microbiol., 2001; 82: 39-43.
11. Miserez, R., Frey, J., Buogo, C., Capaul, S., Tontis, A., Burnens, A., Nicolet, J.: Detection of α- and ε-toxigenic Clostridium perfringens Type D in sheep and goats using a DNA amplifi cation technique (PCR). Lett. Appl. Microbiol., 1998; 26: 382-386.
12. Miyakawa, M.E.F., Ibarra, C.A., Uzal, F.A.: In vitro effects of Clostridium perfringens type D epsilon toxin on water and ion transport in ovine and caprine intestine. Anaerobe, 2003; 9: 145-149.
13. Layana, J.E., Miyakawa, M.E.F., Uzal, F.A.: Evaluation of different fl uids for detection of Clostridium perfringens type D epsilon toxin in sheep with experimental enterotoxemia. Anaerobe, 2006; 12: 204-206.
14. Garmory, H.S., Chanter, N., French, N.P., Bueschel, D., Songer, J.G., Titball, R.W.: Occurrence of Clostridium perfringens β2-toxin amongst animals, determined using genotyping and subtyping PCR assays. Epidemiol. Infect., 2000; 124: 61-67.
15. Niilo, L.: Clostridium perfringens in animal disease: a review of current knowledge. Can. Vet. J. B., 1980; 21: 141-148.
16. Johansson, A., Aspan, A., Bagge, E., Baverud, V., Engström, B.E., Johansson, K.E.: Genetic diversity of Clostridium perfringens type A isolates from animals, food poisoning outbreaks and sludge. BMC Microbiol., 2006; 6: 1-12.
17. Kadra, B., Guillou, J.P., Popoff, M., Bourlioux, P.: Typing of sheep clinical isolates and identifi cation of enterotoxigenic Clostridium perfringens strains by classical methods and by polymerase chain reaction (PCR). FEMS Immun. Med. Microbiol., 1999; 24: 259-266.
18. Uzal, F.A., Kelly, W.R., Thomas, R., Hornitzky, M., Galea, F.: Comparison of four techniques for the detection of Clostridium perfringens type D epsilon toxin in intestinal contents and other body fl uids of sheep and goats. J. Vet. Diagn. Invest., 2003; 15: 94-99.
19. Yoo, H.S., Lee, S.U., Park, K.Y., Park, Y.H.: Molecular typing and epidemiological survey of prevalence of Clostridium perfringens types by multiplex PCR. J. Clin. Microbiol., 1997; 35: 228-232.
20. Naylor, R.D., Martin, P.K., Barker, L.T.: Detection of Clostridium perfringens toxin by enzyme-linked immunosorbent assay. Res. Vet. Sci., 1997; 63: 101-102.
21. Vaikosen, E.S., Ikhatua, U.J.: Detection of high level of enterotoxin of Clostridum perfringens types C and D in small ruminants in Nigeria. Small. Rum. Res., 2005, 58: 287-290.
22. Baums, C.G., Schotte, U., Amtsberg, G., Goethe R.: Diagnostic multiplex PCR for toxin genotyping of Clostridium perfringens isolates. Vet. Microbiol., 2004; 100: 11-16.
23. Kalender, H., Ertas, H.B., Cetinkaya, B., Muz, A., Arslan, N., Kilic, A.: Typing of isolates of Clostridium perfringens from healthy and diseased sheep by multiplex PCR. Vet. Med. Czech., 2005; 50: 439-442.
24. Manteca, C., Daube, G., Pirson, V., Limbourg, B., Kaeckenbeeck, A., Mainil, J.G.: Bacterial intestinal flora associated with enterotoxaemia in Belgian Blue calves. Vet. Microbiol., 2001; 81: 21-32.
25. Koç, R., Gökçe, H.İ.: Determination of the toxins and biotypes of Clostridium perfringens in diarrhoeic calves in the Kars district of Turkey. T. J. Vet. Anim. Sci., 2007; 31: 1-5.
26. Gökçe, H.İ., Genç, O., Sözmen, M., Gökçe, G.: Determination of Clostridium perfringens toxin-types in sheep with suspected enterotoxemia in Kars Province, Turkey. Turk. J. Vet. Anim. Sci., 2007; 3: 355-360.
27. Bueschel, D.M., Jost, B.H., Billington, S.J., Trinh, H.T., Songer, J.G.: Prevalence of cpb2, encoding beta2 toxin, in Clostridium perfringens field isolates: correlation of genotype with phenotype. Vet. Microbiol., 2003; 94: 121-129.
28. Özcan, C., Gürçay, M.: Enterotoxaemia incidence in small ruminants in Elazi{dot less}ǧ and the surrounding provinces in 1994-1998. Turk. J. Vet. Anim. Sci., 2000; 24: 283-286 (in Turkish with English abstract).