Potential nonpneumococcal confounding of PCR-based determination of serotype in carriage

Journal of Clinical Microbiology

Volumn 50, Issue 9, 2012, Pages 3146-3147

  Carvalho, Maria Da Gloria ^a   Jagero, Geoffrey ^b   Bigogo, Godfrey M ^b   Junghae, Muthoni ^b   Pimenta, Fabiana C ^a   Moura, Iaci ^a   Roundtree, Alexis ^a   Li, Zhongya ^a   Conklin, Laura ^a   Feikin, Daniel R ^b   Breiman, Robert F ^b   Whitney, Cynthia G ^a   Beall, Bernard ^a  

^a NATIONAL CENTER FOR IMMUNIZATION AND RESPIRATORY DISEASES   (United States)

^b CENTERS FOR DISEASE CONTROL AND PREVENTION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMPLICON; BACTERIAL STRAIN; CONTROLLED STUDY; DOLOSIGRANULUM PIGRUM; GENE SEQUENCE; HUMAN; LETTER; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SEROTYPE; SPECIES DIVERSITY; STREPTOCOCCUS AUSTRALIS; STREPTOCOCCUS CRISTATUS; STREPTOCOCCUS GORDONII; STREPTOCOCCUS INFANTIS; STREPTOCOCCUS INTESTINALIS; STREPTOCOCCUS MITIS; STREPTOCOCCUS OLIGOFERMENTANS; STREPTOCOCCUS ORALIS; STREPTOCOCCUS PARASANGUINIS; STREPTOCOCCUS PERORIS; STREPTOCOCCUS PSEUDOPNEUMONIAE; STREPTOCOCCUS SALIVARIUS; STREPTOCOCCUS SANGUINIS; STREPTOCOCCUS SINENSIS; STREPTOCOCCUS VESTIBULARIS;

BACTERIOLOGICAL TECHNIQUES; CARRIER STATE; DIAGNOSTIC ERRORS; GENOTYPE; HUMANS; MOLECULAR DIAGNOSTIC TECHNIQUES; PNEUMOCOCCAL INFECTIONS; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC; SEROTYPING; STREPTOCOCCUS PNEUMONIAE;

EID: 84865190595     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.01505-12     Document Type: Letter

References (5)

1. Carvalho MDG, et al. 2012. PCR assessment of carriage among population with high HIV prevalence: frequent colonization, multiple serotypes, and intra-serotype variability within wzy PCR-serotyping target, abstr. 306. 8th Int. Symp. Pneumococci Pneumococcal Dis. Iguacu Falls, Brazil, 11 to 15 March 2012.
2. Carvalho MDG, et al. 2010. Revisiting pneumococcal carriage using broth-enrichment and PCR techniques for enhanced detection of carriage and serotypes. J. Clin. Microbiol. 48:1611-1618.
3. Carvalho MDG, et al. 2007. Evaluation and improvement of real-time PCR assays targeting lytA, ply, and psaA genes for detection of pneumococcal DNA. J. Clin. Microbiol. 45:2460 -2466.
4. Dowson CG, et al. 1989. Horizontal transfer of penicillin-binding protein genes in penicillin-resistant clinical isolates of Streptococcus pneumoniae. Proc. Natl. Acad. Sci. U. S. A. 86:8842- 8846.
5. Yoshida Y, et al. 2008. Molecular and antigenic characterization of a Streptococcus oralis coaggregation receptor polysaccharide by carbohydrate engineering in Streptococcus gordonii. J. Biol. Chem. 283:12654-12664.