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Volumn 7, Issue 8, 2012, Pages

Deep sequencing reveals complex spurious transcription from transiently transfected plasmids

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY RNA; DOUBLE STRANDED RNA; LUCIFERASE; PLASMID DNA;

EID: 84865080613     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0043283     Document Type: Article
Times cited : (28)

References (28)
  • 3
  • 4
    • 0024582782 scopus 로고
    • The phosphorylation state of eucaryotic initiation factor 2 alters translational efficiency of specific mRNAs
    • Kaufman RJ, Davies MV, Pathak VK, Hershey JW, (1989) The phosphorylation state of eucaryotic initiation factor 2 alters translational efficiency of specific mRNAs. Mol Cell Biol 9: 946-958.
    • (1989) Mol Cell Biol , vol.9 , pp. 946-958
    • Kaufman, R.J.1    Davies, M.V.2    Pathak, V.K.3    Hershey, J.W.4
  • 5
    • 0025966601 scopus 로고
    • Enhancement of expression of exogenous genes by 2-aminopurine. Regulation at the post-transcriptional level
    • Kalvakolanu DV, Bandyopadhyay SK, Tiwari RK, Sen GC, (1991) Enhancement of expression of exogenous genes by 2-aminopurine. Regulation at the post-transcriptional level. J Biol Chem 266: 873-879.
    • (1991) J Biol Chem , vol.266 , pp. 873-879
    • Kalvakolanu, D.V.1    Bandyopadhyay, S.K.2    Tiwari, R.K.3    Sen, G.C.4
  • 6
    • 0026537862 scopus 로고
    • A pitfall of using a second plasmid to determine transfection efficiency
    • Farr A, Roman A, (1992) A pitfall of using a second plasmid to determine transfection efficiency. Nucleic Acids Res 20: 920.
    • (1992) Nucleic Acids Res , vol.20 , pp. 920
    • Farr, A.1    Roman, A.2
  • 7
    • 0028362078 scopus 로고
    • Inhibition of reporter gene expression in mammalian cells. Effects of distinct carcinogen lesions in DNA
    • Sorscher DH, Cordeiro-Stone M, (1994) Inhibition of reporter gene expression in mammalian cells. Effects of distinct carcinogen lesions in DNA. Carcinogenesis 15: 1093-1096.
    • (1994) Carcinogenesis , vol.15 , pp. 1093-1096
    • Sorscher, D.H.1    Cordeiro-Stone, M.2
  • 8
    • 0030920768 scopus 로고    scopus 로고
    • DNA transfection to study translational control in mammalian cells
    • Kaufman RJ, (1997) DNA transfection to study translational control in mammalian cells. Methods 11: 361-370.
    • (1997) Methods , vol.11 , pp. 361-370
    • Kaufman, R.J.1
  • 9
    • 0033571204 scopus 로고    scopus 로고
    • The antiviral enzymes PKR and RNase L suppress gene expression from viral and non-viral based vectors
    • Terenzi F, deVeer MJ, Ying H, Restifo NP, Williams BR, et al. (1999) The antiviral enzymes PKR and RNase L suppress gene expression from viral and non-viral based vectors. Nucleic Acids Res 27: 4369-4375.
    • (1999) Nucleic Acids Res , vol.27 , pp. 4369-4375
    • Terenzi, F.1    deVeer, M.J.2    Ying, H.3    Restifo, N.P.4    Williams, B.R.5
  • 10
    • 34547865075 scopus 로고    scopus 로고
    • pGL4 vectors: A new generation of luciferase reporter vectors
    • Paguio A, Almond B, Fan F, Stecha P, Garwin D, et al. (2005) pGL4 vectors: A new generation of luciferase reporter vectors. Promega Notes 89: 7-10.
    • (2005) Promega Notes , vol.89 , pp. 7-10
    • Paguio, A.1    Almond, B.2    Fan, F.3    Stecha, P.4    Garwin, D.5
  • 11
    • 45049083065 scopus 로고    scopus 로고
    • Normalizing genetic reporter assays: approaches and considerations for increasing consistency and statistical significance
    • Schagat T, Paguio A, Kopish K (2007) Normalizing genetic reporter assays: approaches and considerations for increasing consistency and statistical significance. Cell Notes: 9-12.
    • (2007) Cell Notes , pp. 9-12
    • Schagat, T.1    Paguio, A.2    Kopish, K.3
  • 12
    • 12244268013 scopus 로고    scopus 로고
    • Promoterless luciferase reporter gene is transactivated by basic helix-loop-helix transcription factors
    • Hong SJ, Chae H, Kim KS (2002) Promoterless luciferase reporter gene is transactivated by basic helix-loop-helix transcription factors. Biotechniques 33: 1236-1238, 1240.
    • (2002) Biotechniques , vol.33
    • Hong, S.J.1    Chae, H.2    Kim, K.S.3
  • 13
    • 12244298928 scopus 로고    scopus 로고
    • pRL-TK induction can cause misinterpretation of gene promoter activity
    • Osborne SA, Tonissen KF, (2002) pRL-TK induction can cause misinterpretation of gene promoter activity. Biotechniques 33: 1240-1242.
    • (2002) Biotechniques , vol.33 , pp. 1240-1242
    • Osborne, S.A.1    Tonissen, K.F.2
  • 14
    • 0024208947 scopus 로고
    • Many transcription factors interact synergistically with steroid receptors
    • Schule R, Muller M, Kaltschmidt C, Renkawitz R, (1988) Many transcription factors interact synergistically with steroid receptors. Science 242: 1418-1420.
    • (1988) Science , vol.242 , pp. 1418-1420
    • Schule, R.1    Muller, M.2    Kaltschmidt, C.3    Renkawitz, R.4
  • 15
    • 0034854288 scopus 로고    scopus 로고
    • Activation of pRL-TK by 12S E1A oncoprotein: drawbacks of using an internal reference reporter in transcription assays
    • Thavathiru E, Das GM (2001) Activation of pRL-TK by 12S E1A oncoprotein: drawbacks of using an internal reference reporter in transcription assays. Biotechniques 31: 528-530, 532.
    • (2001) Biotechniques , vol.31
    • Thavathiru, E.1    Das, G.M.2
  • 16
    • 0034056007 scopus 로고    scopus 로고
    • Cryptic enhancer elements in luciferase reporter vectors respond to the osteoblast-specific transcription factor Osf2/Cbfa1
    • Thirunavukkarasu K, Miles RR, Halladay DL, Onyia JE, (2000) Cryptic enhancer elements in luciferase reporter vectors respond to the osteoblast-specific transcription factor Osf2/Cbfa1. Biotechniques 28: 506-510.
    • (2000) Biotechniques , vol.28 , pp. 506-510
    • Thirunavukkarasu, K.1    Miles, R.R.2    Halladay, D.L.3    Onyia, J.E.4
  • 17
    • 50649094968 scopus 로고    scopus 로고
    • Firefly luciferase gene contains a cryptic promoter
    • Vopalensky V, Masek T, Horvath O, Vicenova B, Mokrejs M, et al. (2008) Firefly luciferase gene contains a cryptic promoter. RNA 14: 1720-1729.
    • (2008) RNA , vol.14 , pp. 1720-1729
    • Vopalensky, V.1    Masek, T.2    Horvath, O.3    Vicenova, B.4    Mokrejs, M.5
  • 18
    • 70449699603 scopus 로고    scopus 로고
    • Factors modulating expression of Renilla luciferase from control plasmids used in luciferase reporter gene assays
    • Shifera AS, Hardin JA, (2010) Factors modulating expression of Renilla luciferase from control plasmids used in luciferase reporter gene assays. Anal Biochem 396: 167-172.
    • (2010) Anal Biochem , vol.396 , pp. 167-172
    • Shifera, A.S.1    Hardin, J.A.2
  • 19
    • 0022259993 scopus 로고
    • Gene expression from both intronless and intron-containing Rous sarcoma virus clones is specifically inhibited by anti-sense RNA
    • Chang LJ, Stoltzfus CM, (1985) Gene expression from both intronless and intron-containing Rous sarcoma virus clones is specifically inhibited by anti-sense RNA. Mol Cell Biol 5: 2341-2348.
    • (1985) Mol Cell Biol , vol.5 , pp. 2341-2348
    • Chang, L.J.1    Stoltzfus, C.M.2
  • 20
    • 13844251883 scopus 로고    scopus 로고
    • Inhibitory effects associated with use of modified Photinus pyralis and Renilla reniformis luciferase vectors in dual reporter assays and implications for analysis of ISGs
    • Ghazawi I, Cutler SJ, Low P, Mellick AS, Ralph SJ, (2005) Inhibitory effects associated with use of modified Photinus pyralis and Renilla reniformis luciferase vectors in dual reporter assays and implications for analysis of ISGs. J Interferon Cytokine Res 25: 92-102.
    • (2005) J Interferon Cytokine Res , vol.25 , pp. 92-102
    • Ghazawi, I.1    Cutler, S.J.2    Low, P.3    Mellick, A.S.4    Ralph, S.J.5
  • 21
  • 22
    • 10744219596 scopus 로고    scopus 로고
    • Specific inhibition of gene expression using a stably integrated, inducible small-interfering-RNA vector
    • van de Wetering M, Oving I, Muncan V, Pon Fong MT, Brantjes H, et al. (2003) Specific inhibition of gene expression using a stably integrated, inducible small-interfering-RNA vector. EMBO Rep 4: 609-615.
    • (2003) EMBO Rep , vol.4 , pp. 609-615
    • van de Wetering, M.1    Oving, I.2    Muncan, V.3    Pon Fong, M.T.4    Brantjes, H.5
  • 23
    • 0035925583 scopus 로고    scopus 로고
    • Simple and efficient vectors for retrofitting BACs and PACs with mammalian neoR and EGFP marker genes
    • Kaname T, Huxley C, (2001) Simple and efficient vectors for retrofitting BACs and PACs with mammalian neoR and EGFP marker genes. Gene 266: 147-153.
    • (2001) Gene , vol.266 , pp. 147-153
    • Kaname, T.1    Huxley, C.2
  • 24
    • 82455202433 scopus 로고    scopus 로고
    • dsRNA expression in the mouse elicits RNAi in oocytes and low adenosine deamination in somatic cells
    • Nejepinska J, Malik R, Filkowski J, Flemr M, Filipowicz W, et al. (2012) dsRNA expression in the mouse elicits RNAi in oocytes and low adenosine deamination in somatic cells. Nucleic Acids Res 40: 399-413.
    • (2012) Nucleic Acids Res , vol.40 , pp. 399-413
    • Nejepinska, J.1    Malik, R.2    Filkowski, J.3    Flemr, M.4    Filipowicz, W.5
  • 25
    • 62349130698 scopus 로고    scopus 로고
    • Ultrafast and memory-efficient alignment of short DNA sequences to the human genome
    • Langmead B, Trapnell C, Pop M, Salzberg SL, (2009) Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol 10: R25.
    • (2009) Genome Biol , vol.10
    • Langmead, B.1    Trapnell, C.2    Pop, M.3    Salzberg, S.L.4
  • 26
    • 79955756836 scopus 로고    scopus 로고
    • Design and validation issues in RNA-seq experiments
    • Fang Z, Cui X, (2011) Design and validation issues in RNA-seq experiments. Brief Bioinform 12: 280-287.
    • (2011) Brief Bioinform , vol.12 , pp. 280-287
    • Fang, Z.1    Cui, X.2
  • 27
    • 67649595548 scopus 로고    scopus 로고
    • Limitations and possibilities of small RNA digital gene expression profiling
    • Linsen SE, de Wit E, Janssens G, Heater S, Chapman L, et al. (2009) Limitations and possibilities of small RNA digital gene expression profiling. Nat Methods 6: 474-476.
    • (2009) Nat Methods , vol.6 , pp. 474-476
    • Linsen, S.E.1    de Wit, E.2    Janssens, G.3    Heater, S.4    Chapman, L.5
  • 28
    • 70350569212 scopus 로고    scopus 로고
    • Endo-siRNAs depend on a new isoform of loquacious and target artificially introduced, high-copy sequences
    • Hartig JV, Esslinger S, Bottcher R, Saito K, Forstemann K, (2009) Endo-siRNAs depend on a new isoform of loquacious and target artificially introduced, high-copy sequences. EMBO J 28: 2932-2944.
    • (2009) EMBO J , vol.28 , pp. 2932-2944
    • Hartig, J.V.1    Esslinger, S.2    Bottcher, R.3    Saito, K.4    Forstemann, K.5


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.