Assaying proline hydroxylation in recombinant collagen variants by liquid chromatography-mass spectrometry

BMC Biotechnology

Volumn 12, Issue , 2012, Pages

  Chan, S W P ^a   Greaves, John ^b   Da Silva, Nancy A ^a   Wang, Szu Wen ^a  

^a University of California at Irvine   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Hydroxyproline; LC MS assay; Liquid chromatography mass spectrometry; Recombinant collagen

Indexed keywords

4 HYDROXYPROLINES; BIOPHYSICAL STUDIES; CELL MATRIX INTERACTIONS; CONVENTIONAL METHODS; DERIVATIZATIONS; EXPRESSION SYSTEM; EXTRACELLULAR MATRICES; HYDROXYPROLINE; LC-MS METHODS; LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY; LOW LEVEL; NATIVE COLLAGEN; POST-TRANSLATIONAL MODIFICATIONS; PROLYL-4-HYDROXYLASE; RECOMBINANT EXPRESSION; TISSUE REGENERATION; YEAST EXPRESSION;

AMINO ACIDS; CHROMATOGRAPHY; COLLAGEN; GENE EXPRESSION; OLIGONUCLEOTIDES; TISSUE; YEAST;

HYDROXYLATION;

COLLAGEN; COLLAGEN TYPE 3; HYDROXYPROLINE; OLIGONUCLEOTIDE; PROCOLLAGEN PROLINE 2 OXOGLUTARATE 4 DIOXYGENASE; PROLINE; PROTEIN VARIANT; RECOMBINANT COLLAGEN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ACCURACY; AMINO ACID ANALYSIS; ANALYTIC METHOD; ARTICLE; ASSAY; GENE EXPRESSION; HYDROXYLATION; LIQUID CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY MASS SPECTROMETRY; MASS SPECTROMETRY; NONHUMAN; SACCHAROMYCES CEREVISIAE;

CHROMATOGRAPHY, LIQUID; COLLAGEN; HUMANS; HYDROXYLATION; MASS SPECTROMETRY; PROLINE; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE;

BACTERIA (MICROORGANISMS); SACCHAROMYCES CEREVISIAE;

EID: 84865061422     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-12-51     Document Type: Article

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