Evaluation and comparison of decalcification agents on the human teeth

Journal of Oral and Maxillofacial Pathology

Volumn 16, Issue 2, 2012, Pages 222-227

  Sanjai, Karpagaselvi ^a   Kumarswamy, Jayalakshmi ^a   Patil, Archana ^a   Papaiah, Lokesh ^a   Jayaram, Srinivas ^a   Krishnan, Lakshmi ^a  

^a Vydehi Institute of Dental Sciences and Research Centre   (India)

Author keywords

10 formic acid; 5 nitric acid; 5 trichloracetic acid; formalin nitric acid; neutral EDTA decalcifying solution; Perenyi's fluid; pulp tissue integrity; teeth decalcification

Indexed keywords

EID: 84864987008     PISSN: 0973029X     EISSN: 1998393X     Source Type: Journal    
DOI: 10.4103/0973-029X.99070     Document Type: Article

References (19)

1. Olympic charter published by International Olympic committee, Lausanne, Switzerland; 2011. p. 9-21.
2. Cook SF, Ezra-Cohn HE. A comparison of methods for decalcifying bone. J Histochem Cytochem 1962;10:560-63.
3. Drury RA, Wallington EA. Carleton's Histological Technique. 5 th ed. Oxford: Oxford University Press; 1980. p. 199-205.
4. Mattuella LG, Bento LW, Vier-Pelisser FV, Araiyo FB, Fossati AC. Comparative analysis of two fixating and two decalcifying solutions for processing of human primary teeth with inactive dentin carious lesion. Rev Odonto Ciênc. 2007;22:99-105.
5. Callis MG. Bone. In: Bancroft JD, Gamble M, editors. Theory and practice of histological techniques. 6 th ed. Philadelphia: Churchill Livingstone; 2008. p. 333-63.
6. Singh S, Sircar K. Evaluation of efficacy of various chemicals for decalcification of dental hard tissues-An in-vitro study. J Orofac Sci 2010;1:5-10.
7. Culling CF, Allison RT, Barr WT. Cellular pathology technique. 4 th ed. London: Butterworths; 1985. p. 408-30.
8. Verdenius HH, Alma L. A quantitative study of decalcification methods in histology. J Clin Pathol 1958;11:229-36.
9. Birkedal-Hansen H. Kinetics of acid demineralization in histologic technique. J Histochem Cytochem 1974;22:434-41.
10. Mawhinney WH, Richardson E, Malcolm AJ. Control of rapid nitric acid decalcification. J Clin Pathol 1984;37:1409-13.
11. Zappa J, Cieslik-Bielecka A, Adwent M, Cieslik T, Sabat D. Comparison of different decalcification methods to hard teeth tissues morphological analysis. Dent Med Probl 2005;42:21-6.
12. Waerhaug J. Decalcification of bone and teeth under vacuum-a rapid method for producing hard tissue preparations. J Dent Res 1949;28:525.
13. Vongsavan N, Matthews B, Harrison GK. Decalcification of teeth in a microwave oven. Histochem J 1990;22:377-80.
14. Pitol DL, Caetano FH, Lunardi LO. Microwave-induced fast decalcification of rat bone for electron microscopic analysis: An ultrastructural and cytochemical study. Braz Dent J 2007;18:153-7.
15. Wijnbergen M, van Mullem PJ. Effect of histological decalcifying agents on number and stainability of gram-positive bacteria. J Dent Res 1987;66:1029-31.
16. Bourque WT, Gross M, Hall BK. A histological processing techniq ue that preserves the integrity of calcified tissues (bone, enamel), yolky amphibian embryos, and growth factor antigens in skeletal tissue. J Histochem Cytochem 1993;41:1429-34.
17. Coleman EJ, Desalva SJ. Rapid decalcification for histochemistry. J Dent Res 1966;45:1237.
18. Warshawsky H, Moore G. A technique for the fixation and decalcification of rat incisors for electron microscopy. J Histochem Cytochem 1967;15:542-9.
19. Smith CE. Effect of glutaraldehyde and decalcifying agents on acid phosphomonoester hydrolase activity in the enamel organ of the rat incisor: A biochemical study comparing enamel organ with liver. J Histochem Cytochem 1980;28:689-99.