Characterization of a novel DNA polymerase activity assay enabling sensitive, quantitative and universal detection of viable microbes

Nucleic Acids Research

Volumn 40, Issue 14, 2012, Pages

  Zweitzig, Daniel R ^a   Riccardello, Nichol M ^a   Sodowich, Bruce I ^a   O'Hara, S Mark ^a  

^a Zeus Scientific Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE;

ARTICLE; BACTERIAL GROWTH; BACTERIAL VIABILITY; BACTERIUM COLONY; BACTERIUM CULTURE; BACTERIUM DETECTION; CELL LYSATE; COLONY FORMING UNIT; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME PURIFICATION; ESCHERICHIA COLI; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; STAPHYLOCOCCUS AUREUS;

BACTERIA; DEOXYCYTOSINE NUCLEOTIDES; DNA-DIRECTED DNA POLYMERASE; ENZYME ASSAYS; MICROBIAL VIABILITY; POLYMERASE CHAIN REACTION;

EID: 84864913941     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gks316     Document Type: Article

References (26)

1. Rothwell, P.J. and Waksman, G. (2005) Structure and mechanism of DNA polymerases. Advan. Prot. Chem., 71, 401-440. (Pubitemid 41446935)
2. Joyce, C.M. and Steitz, T.A. (1994) Function and structure relationships in DNA polymerases. Annu. Rev. Biochem., 63, 777-822. (Pubitemid 24218649)
3. Mar Albà, M. (2001) Replicative DNA polymerases. Genome Biol., 2, 3002.1-3002.4.
4. Lehman, I.R., Bessman, M.J., Simms, E.S. and Kornberg, A. (1958) Enzymatic synthesis of deoxyribonucleic acid. I. preparation of substrates and partial purification of an enzyme from Escherichia coli. J. Biol. Chem., 233, 163-170.
5. Hamilton, S.C., Farchaus, J.W. and Davis, M.C. (2001) DNA polymerases as engines for biotechnology. BioTechniques, 31, 370-376, 378-380, 382-383.
6. Tarantino, P.M., Zhi, C., Wright, G.E. and Brown, N.C. (1999) Inhibitors of DNA polymerase III as novel antimicrobial agents against gram-positive eubacteria. Antimicrob. Agents Chemother., 43, 1982-1987. (Pubitemid 29395196)
7. Kuhl, A., Svenstrup, N., Ladel, C., Otteneder, M., Binas, A., Schiffer, G., Brands, M., Lampe, T., Ziegelbauer, K., Rü bsamen-Waigmann, H. et al. (2005) Biological characterization of novel inhibitors of the gram-positive DNA polymerase IIIC enzyme. Antimicrob. Agents Chemother., 49, 987-995. (Pubitemid 40327714)
8. Lange, S.S., Takata, K. and Wood, R.D. (2011) DNA polymerases and cancer. Nat. Rev. Cancer, 11, 96-110.
9. Martin, S.A., McCabe, N., Mullarkey, M., Cummins, R., Burgess, D.J., Nakabeppu, Y., Oka, S., Kay, E., Lord, C.J. and Ashworth, A. (2010) DNA polymerases as potential therapeutic targets for cancers deficient in the DNA mismatch repair proteins MSH2 or MLH1. Cancer Cell, 17, 235-248.
10. Naviaux, R.K., Markusic, D., Barshop, B.A., Nyhan, W.L. and Haas, R.H. (1999) Sensitive assay for mitochondrial DNA polymerase gamma. Clin. Chem., 45, 1725-1733. (Pubitemid 29477328)
11. Richardson, C.C., Schildkraut, C.L., Vasken Aposhian, H. and Kornberg, A. (1964) Enzymatic synthesis of deoxyribonucleic acid. J. Biol. Chem., 239, 222-232.
12. Griep, M.A. (1995) Fluorescence recovery assay: a continuous assay for processive DNA polymerase applied specifically to DNA polymerase III holoenzyme. Anal. Biochem., 232, 180-189. (Pubitemid 26010257)
13. Seville, M., West, A.B., Cull, M.G. and McHenry, C.S. (1996) Fluorometric assay for DNA polymerases and reverse transcriptase. Biotechniques, 21, 664, 666, 668, 670, 672.
14. Tveit, H. and Kristensen, T. (2001) Fluorescence-based DNA polymerase assay. Anal. Biochem., 289, 96-98. (Pubitemid 32121574)
15. Yu, L., Hu, G. and Howells, L. (2002) Fluorescence-based, high-throughput DNA polymerase assay. Biotechniques, 33, 938-941. (Pubitemid 35168080)
16. Ma, C., Tang, Z., Wang, K., Tan, W., Li, J., Li, W., Li, Z., Yang, X., Li, H. and Liu, L. (2006) Real-time monitoring of DNA polymerase activity using molecular beacon. Anal. Biochem., 353, 141-143.
17. Luo, X. and Hsing, I. (2011) Immobilization-free electrochemical DNA polymerase assay. Electroanalysis, 23, 923-926.
18. Banin, S., Wilson, S. and Stanley, C. (2007) The LiMA technology: measurement of ATP on a nucleic acid testing platform. Clin. Chem., 53, 2034-2036. (Pubitemid 350036066)
19. Toji, L. and Cohen, S.S. (1969) The enzymatic termination of polydeoxynucleotides by 2'3'-dideoxyadenosine triphosphate. Proc. Natl Acad. Sci. USA, 63, 871-877.
20. Sanger, F., Nicklen, S. and Coulson, R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl Acad. Sci. USA, 74, 5463-5467.
21. Davey, H.M. (2011) Life, death, and in-between: meanings and methods in microbiology. Appl. Environ. Microbiol., 77, 5571-5576.
22. Rappe, M.S. and Giovannoni, S.J. (2003) The uncultured microbial majority. Annu. Rev. Microbiol., 57, 369-394. (Pubitemid 37392948)
23. Riley, B. (2004) Rapid microbiology methods in the pharmaceutical industry. Am. Pharm. Rev., 7, 28-31. (Pubitemid 38628351)
24. Keer, J.T. and Birch, L. (2003) Molecular methods for the assessment of bacterial viability. J. Microbiol. Meth., 53, 175-183. (Pubitemid 36332352)
25. Masters, C., Shallcross, J. and Mackey, B. (1994) Effect of stress treatments on the detection of Listeria monocytogenes and enterotoxigenic Escherichia coli by the polymerase chain reaction. J. Appl. Bacteriol., 77, 73-79. (Pubitemid 24225096)
26. Sheridan, G.E., Masters, C.I., Shallcross, J.A. and Mackey, B.M. (1998) Detection of mRNA by reverse transcription-PCR as an indicator of viability in Escherichia coli cells. Appl. Environ. Microbiol., 64, 1313-1318. (Pubitemid 28188389)