Increase in flavan-3-ols by silencing flavonol synthase mRNA affects the transcript expression and activity levels of antioxidant enzymes in tobacco

Plant Biology

Volumn 14, Issue 5, 2012, Pages 725-733

  Mahajan, M ^a   Joshi, R ^a   Gulati, A ^a   Yadav, S K ^a  

^a CSIR INSTITUTE OF HIMALAYAN BIORESOURCE TECHNOLOGY   (India)

Author keywords

Antioxidant; Catechin; Flavan 3 ols; Flavonol synthase; Nicotiana

Indexed keywords

ANTIOXIDANT; BIOCHEMICAL COMPOSITION; ENZYME ACTIVITY; GENE EXPRESSION; METABOLITE; OXYGEN; PHYSIOLOGICAL RESPONSE; RADICAL; SCAVENGING (CHEMISTRY); TOBACCO;

NICOTIANA; NICOTIANA TABACUM;

ANTIOXIDANT; CATECHIN; FLAVAN-3-OL; FLAVONOID; FLAVONOL SYNTHASE; MESSENGER RNA; OXIDOREDUCTASE; VEGETABLE PROTEIN;

DRUG EFFECTS; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENE SILENCING; GENETICS; METABOLISM; TOBACCO; TRANSGENIC PLANT;

ANTIOXIDANTS; CATECHIN; FLAVONOIDS; GENE EXPRESSION REGULATION, PLANT; GENE SILENCING; METABOLIC NETWORKS AND PATHWAYS; OXIDOREDUCTASES; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; RNA, MESSENGER; TOBACCO;

EID: 84864741116     PISSN: 14358603     EISSN: 14388677     Source Type: Journal    
DOI: 10.1111/j.1438-8677.2011.00550.x     Document Type: Article

References (31)

1. Carlberg I., Mannervik B. (1985) Glutathione reductase. Methods in Enzymology, 113, 484-490.
2. Deluc L., Bogs J., Walker A.R., Ferrier T., Decendit A., Merillon J-M., Robinson S.P., Barrieu F. (2008) The transcription factor VvMYB5b contributes to the regulation of anthocyanin and proanthocyanidin biosynthesis in developing grape berries. Plant Physiology, 147, 2041-2053.
3. Dhindsa R.S., Plumb-Dhindsa P., Thorpe T.A. (1981) Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. Journal of Experimental Botany, 32, 93-101.
4. Diaz Napal G.N., Carpinella M.C., Palacios S.M. (2009) Antifeedant activity of ethanolic extract from Flourensia oolepis and isolation of pinocembrin as its active principle compound. Bioresource Technology, 100, 3669-3673.
5. Gossett D.R., Millhollon E.P., Lucas M.C. (1994) Antioxidant response to NaCl stress in salt-tolerant and salt-sensitive cultivars of cotton. Crop Science, 34, 706-714.
6. Habig W.H., Pabst M.J., Jakoby W.B. (1974) Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. Journal of Biological Chemistry, 246, 7130-7139.
7. Hara Y., Luo S.J., Wickremashinghe R.L., Yamanishi T. (1995) Botany (of tea). Food Reviews International, 11, 371-374.
8. Hernández I., Alegre L., Van Breusegem F., Munné-Bosch S. (2009) How relevant are flavonoids as antioxidants in plants? Trends in Plant Science, 14, 125-132.
9. Horsch R.B., Fry J.E., Hoffmann N.L., Eicholtz D., Rogers S.G., Fraley R.T. (1985) A simple method for transferring genes into plants. Science, 227, 1229-1231.
10. Izaguirre M.M., Mazza C.A., Svatos A., Baldwin I.T., Ballaré C.L. (2007) Solar ultraviolet-B radiation and insect herbivory trigger partially overlapping phenolic responses in Nicotiana attenuata and Nicotiana longiflora. Annals of Botany, 99, 103-109.
11. Jayakumar K., Jaleel C.A., Vijayarengan P. (2007) Changes in growth, biochemical constituents and antioxidant potentials in radish (Raphanus sativus L.) under cobalt stress. Turkish Journal of Biology, 31, 27-136.
12. Koes R.E., Quattrocchio F., Mol J.N.M. (1993) The flavonoid biosynthetic pathway in plants: function and evolution. Bioessays, 16, 123-132.
13. Kubasek W.L., Shirley B.W., McKillop A., Goodman H.M., Briggs W., Ausubel F.M. (1992) Regulation of flavonoid biosynthetic genes in germinating Arabidopsis seedlings. Plant Cell, 4, 1229-1236.
14. Kumar S., Singla-Pareek S.L., Reddy M.K., Sopory S.K. (2003) Glutathione: biosynthesis, homeostasis and its role in abiotic stresses. Journal of Plant Biology, 30, 179-187.
15. Mahajan M., Kumar V., Yadav S.K. (2011) Effect of flavonoid-mediated free IAA regulation on growth and development of in vitro-grown tobacco seedlings. International Journal of Plant Developmental Biology, 5, 42-48.
16. Murashige T., Skoog F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum, 15, 473-497.
17. Nagle D.G., Ferreira D., Zhou Y.D. (2006) Epigallocatechin-3-gallate (EGCG): chemical and biomedical perspectives. Phytochemistry, 67, 1849-1855.
18. Nakano Y., Asada K. (1981) Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplast. Plant Cell Physiology, 22, 867-880.
19. Nakayama T., Yamada M., Osawa T., Kawakishi S. (1993) Suppression of active oxygen-induced cytotoxicity by flavonoids. Biochemistry of Pharmacology, 45, 265-267.
20. Nègre-Salvayre A., Salvayre R. (1992) Quercetin prevents the cytotoxicity of oxidized LDL on lymphoid cell lines. Free Radical Biology and Medicine, 12, 101-106.
21. Pelletier M.K., Burbulis I.E., Winkel-Shirley B. (1999) Disruption of specific flavonoid genes enhances the accumulation of flavonoid enzymes and end-products in Arabidopsis seedlings. Plant Molecular Biology, 40, 45-54.
22. Rabino I., Mancinelli A.L. (1986) Light, temperature, and anthocyanin production. Plant Physiology, 81, 922-924.
23. Rice-Evans C.A., Millier N.J., Paganga G. (1995) Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radical Biology and Medicine, 20, 933-956.
24. Rice-Evans C.A., Miller N.J., Paganga G. (1997) Antioxidant properties of phenolic compounds. Trends in Plant Sciences, 2, 2152-2159.
25. Schroeter H., Heiss C., Balzer J., Kleinbongard P., Keen C.L., Hollenberg N.K., Sies H., Kwik-Uribe C., Schmitz H.H., Kelm M. (2006) (-)-Epicatechin mediates beneficial effects of flavanol-rich cocoa on vascular function in humans. Proceedings of National Academy of Sciences USA, 103, 1024-1029.
26. Sharma V., Gulati A., Ravindranath S.D., Kumar V. (2005) A simple and convenient method for analysis of tea biochemicals by reverse phase HPLC. Journal of Food Composition and Analysis, 18, 583-594.
27. Singh K., Raizada J., Bhardwaj P., Ghawana S., Rani A., Singh H., Kaul K., Kumar S. (2004) 26S rRNA-based internal control gene primer pair for reverse transcription-polymerase chain reaction based quantitative expression studies in diverse plant species. Analytical Biochemistry, 335, 330-333.
28. Wang W., Vinocur B., Altman A. (2003) Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta, 218, 1-14.
29. Winkel-Shirley B. (2002) Biosynthesis of flavonoids and effects of stress. Current Opinion in Plant Biology, 5, 218-223.
30. Woisky R., Salatino A. (1998) Analysis of propolis: some parameters and procedure for chemical quality control. Journal of Agricultural Research, 37, 99-105.
31. Zhang G., Tanakamaru K., Abe J., Morita S. (2007) Influence of waterlogging on some anti-oxidative enzymatic activities of two barley genotypes differing in anoxia tolerance. Acta Physiologia Plantarum, 29, 171-176.