Metabolomics classifies phase of care and identifies risk for mortality in a porcine model of multiple injuries and hemorrhagic shock

Journal of Trauma and Acute Care Surgery

Volumn 73, Issue 2 SUPPL. 1, 2012, Pages

  Lexcen, Daniel R ^a   Lusczek, Elizabeth R ^a   Witowski, Nancy E ^a   Mulier, Kristine E ^a   Beilman, Greg J ^a  

^a UNIVERSITY OF MINNESOTA MEDICAL SCHOOL   (United States)

Author keywords

hemorrhagic shock; Metabolomics; multiple injuries; nuclear magnetic resonance (NMR)

Indexed keywords

GLYCOGEN; PURINE; PYRIMIDINE; TRICARBOXYLIC ACID;

ANIMAL EXPERIMENT; ANIMAL TISSUE; CATABOLISM; CELL DAMAGE; CONFERENCE PAPER; CONTROLLED STUDY; DISEASE ASSOCIATION; ENERGY YIELD; EXPERIMENTAL PIG; FATTY ACID OXIDATION; GLUCONEOGENESIS; HEALTH CARE DELIVERY; HEMORRHAGIC SHOCK; ISCHEMIA; MALE; METABOLITE; METABOLOME; METABOLOMICS; MORTALITY; MULTIPLE TRAUMA; NONHUMAN; PRACTICE GUIDELINE; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE; RESUSCITATION; SERUM; TIME;

ANIMALS; DISEASE MODELS, ANIMAL; MAGNETIC RESONANCE SPECTROSCOPY; MALE; METABOLOMICS; MULTIPLE TRAUMA; RESUSCITATION; RISK FACTORS; SHOCK, HEMORRHAGIC; SWINE;

EID: 84864606638     PISSN: 21630755     EISSN: 21630763     Source Type: Journal    
DOI: 10.1097/TA.0b013e3182609821     Document Type: Conference Paper

References (34)

1. Holcomb JB, McMullin NR, Pearse L, Caruso J,Wade CE, Oetjen-Gerdes L, Champion HR, Lawnick M, Farr W, Rodriguez S. Causes of death in US special operations forces in the global war on terrorism: 2001-2004. Ann Surg. 2007;245:986.
2. Kelly JF, Ritenour AE,McLaughlinDF, BaggKA, ApodacaAN, Mallak CT, Pearse L, Lawnick MM, Champion HR, Wade CE. Injury severity and causes of death from Operation Iraqi Freedom and Operation Enduring Freedom: 2003-2004 versus 2006. J Trauma. 2008;64:S21.
3. Cocchi MN, Kimlin E, Walsh M, Donnino MW. Identification and resuscitation of the trauma patient in shock. Emerg Med Clin North Am. 2007;25:623-642. (Pubitemid 47371058)
4. Moore FA, Nelson T, McKinley BA, Moore EE, Nathens AB, Rhee P, Puyana JC, Beilman GJ, Cohn SM. StO2 study group. Massive transfusion in trauma patients: tissue hemoglobin oxygen saturation predicts poor outcome. J Trauma. 2008;64:1010-1023. (Pubitemid 351521893)
5. Boekstegers P, Weidenhofer S, Pilz G, Werdan K. Peripheral oxygen availability within skeletal muscle in sepsis and septic shock: comparison to limited infection and cardiogenic shock. Infection. 1991;19:317.
6. Odunsi K, Wollman RM, Ambrosone CB, Hutson A, McCann SE, Tammela J, Geisler JP, Miller G, Sellers T, William C, et al. Detection of epithelial ovarian cancer using 1H-NMR-based metabonomics. Int J Cancer. 2005;113:782-788. (Pubitemid 40101260)
7. Serkova N, Fuller TF, Klawitter J, Freise CE, Niemann CU. 1H-NMRbased metabolic signatures of mild and severe ischemia//reperfusion injury in rat kidney transplants. Kidney Int. 2005;67:1142-1151. (Pubitemid 41623341)
8. Averna TA, Kline EE, Smith AY, Sillerud LO. A decrease in 1H nuclear magnetic resonance spectroscopically determined citrate in human seminal fluid accompanies the development of prostate adenocarcinoma. J Urol. 2005;173:433-438.
9. Cohen MJ, Serkova NJ, Wiener-Kronish J, Pittet J, Cohen CU. 1H-NMRbased metabolic signatures of clinical outcomes in trauma patients-beyond lactate and base deficit. J Trauma. 2010;69:31.
10. Mao H, Wang H, Wang B, Liu X, Gao H, Xu M, Zhao H, Deng X, Lin D. Systemic metabolic changes of traumatic critically ill patients revealed by an NMR-based metabonomic approach. J Proteome Res. 2009; 8:5423-5430.
11. Skarda DE, Skard KE, George ME, Beilman GJ. Eight hours of hypotensive versus normotensive resuscitation in a porcine model of controlled hemorrhagic shock. Acad Emerg Med. 2008;15:845.
12. Scribner DM, Witowski NE, Mulier KE, Lusczek ER, Wasiluk KR, Beilman GJ. Liver metabolomic changes identify biochemical pathways in hemorrhagic shock. J Surg Res. 2010;164:e131Ye139.
13. Lusczek ER, Nelson T, Lexcen D, Witowski NE, Mulier KE, Beilman G. Urine metabolomics in hemorrhagic shock: normalization of urine in the face of changing intravascular fluid volume and perterbations in metabolism. J Bioanal Biomed. 2011;03:38-48.
14. Mulier KE, Greenberg JG, Beilman GJ. Hypercoagulability in porcine hemorrhagic shock is present early after trauma and resuscitation. J Surg Res. 2011. In press.
15. Holcomb JB. Effect of dry fibrin sealant dressings versus gauze packing on blood loss in grade V liver injuries in resuscitated swine. J Trauma. 1999;46:49.
16. Beckonert O, Keun HC, Ebbels TMD, Bundy J, Holmes E, Lindon JC, Nicholson JK. Metabolic profiling, metabolomic and metabonomic procedures for NMR spectroscopy of urine, plasma, serum and tissue extracts. Nature Protoc. 2007;2:2692-2703.
17. Weljie AM. Targeted profiling: quantitative analysis of 1H NMR metabolomics data. Anal Chem. 2006;78:4430.
18. Piotto M. Metabolic characterization of primary human colorectal cancers using high resolution magic angle spinning 1H magnetic resonance spectroscopy. Metabolomics. 2009;5:292.
19. Pisarenko O, Studneva I, Khlopkov V, Solomatina E, Ruuge E. An assessment of anaerobic metabolism during ischemia and reperfusion in isolated guinea pig heart. Biochim Biophys Acta. 1988;934:55-63.
20. Wiesner RJ, Deussen A, Borst M, Schrader J, Grieshaber MK. Glutamate degradation in the ischemic dog heart: contribution to anaerobic energy production. J Mol Cell Cardiol. 1989;21:49.
21. Grum CM, Simon RH, Dantzker DR, Fox IH. Evidence for adenosine triphosphate degradation in critically-ill patients. Chest. 1985;88:763.
22. Way CWV, Dhar A, Reddy R, Evans L,Wogahn B, Helling TS. Changes in adenine nucleotides during hemorrhagic shock and reperfusion. J Surg Res. 1996;66:159.
23. McCord JM. Oxygen-derived free radicals in postischemic tissue injury. N Engl J Med. 1985;312:159-163. (Pubitemid 15192863)
24. Ar'Rajab A, Dawidson I, Fabia R. Reperfusion injury. New Horiz. 1996;4:224-234. (Pubitemid 26171431)
25. Marklund N, Ostman B, Nalmo L, Persson L, Hillered L. Hypoxanthine, uric acid and allantoin as indicators of in vivo free radical reactions. description of a HPLC method and human brain microdialysis data. Acta Neurochir. 2000;142:1135.
26. Winkler B, Rathgeb I, Steele R, Altszuler N. Conversion of glycerol to glucose in the normal dog. Am J Physiol. 1970;219:497.
27. Egdahl RH. The importance of the endocrine and metabolic responses to shock and trauma. Crit Care Med. 1977;5:257.
28. Kiewert C, Mdzinarishvili A, Hartmann J, Bickel U, Klein J. Metabolic and transmitter changes in core and penumbra after middle cerebral artery occlusion in mice. Brain Res. 2010;1312:101.
29. Ungerstedt U. MicrodialysisVa new technique for monitoring local tissue events in the clinic. Acta Anaesthesiol Scand Suppl. 1997;110:123.
30. Klein J. Membrane breakdown in acute and chronic neurodegeneration: focus on choline-containing phospholipids. J Neural Transm. 2000;107:1027.
31. Alfieri RR, Bonelli MA, Cavazzoni A, Brigotti M, Fumarola C, Sestili P, Mozzoni P, De Palma G, Mutti A, Camicelli D. Creatine as a compatible osmolyte in muscle cells exposed to hypertonic stress. J Physiol (Lond) . 2006;576:391.
32. Niemann CU, Choi S, Behrends M, Hirose R, Noh J, Coatney JL, Roberts JP, Serkova NJ, Mahr JJ. Mild hypothermia protects obese rats from fulminant hepatic necrosis induced by ischemia-reperfusion. Surgery. 2006;140:404-412. (Pubitemid 44262368)
33. Ishii T, Yasuda K, Akatsuka A, Hino O, Hartman PS, Ishii N. A mutation in the SDHC gene of complex II increases oxidative stress, resulting in apoptosis and tumorigenesis. Cancer Res. 2005;65:203.
34. Vejchapipat P, Williams SR, Spitz L, Pierro A. Intestinal metabolism after ischemia-reperfusion. J Pediatr Surg. 2000;35:759-764. (Pubitemid 30247030)