Step-by-step strategy for protein enrichment and proteome characterisation of extracellular polymeric substances in wastewater treatment systems

Applied Microbiology and Biotechnology

Volumn 95, Issue 3, 2012, Pages 767-776

  Silva, Ana F ^a,b   Carvalho, Gilda ^c   Soares, Renata ^b   Coelho, Ana V ^b   Crespo, M Teresa Barreto ^a,b,d  

^a IBET   (Portugal)

^b INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA   (Portugal)

^c UNIVERSIDADE NOVA DE LISBOA   (Portugal)

^d IBET   (Portugal)

Author keywords

Activated sludge; Extracellular polymeric substances (EPS); Extracellular proteins; Protein identification; SDS PAGE; Wastewater treatment plants

Indexed keywords

ACTIVATED SLUDGE; EXTRACELLULAR POLYMERIC SUBSTANCES; EXTRACELLULAR PROTEINS; PROTEIN IDENTIFICATION; SDS-PAGE; WASTEWATER TREATMENT PLANTS;

ABSORPTION; ACETONE; CENTRIFUGATION; DIALYSIS; DIALYSIS MEMBRANES; ELECTROPHORESIS; INORGANIC ACIDS; MASS SPECTROMETRY; MEMBRANE FOULING; POLYMERS; PRECIPITATION (CHEMICAL); SODIUM; ULTRAFILTRATION;

PROTEINS;

ACETONE; PERCHLORIC ACID; POLYACRYLIC ACID; POLYMER; POLYOL; POLYSACCHARIDE; PROTEOME; TRICHLOROACETIC ACID;

ACTIVATED SLUDGE; BIOMASS; BIOREACTOR; ELECTROKINESIS; HYDROPHOBICITY; MASS SPECTROMETRY; MEMBRANE; PRECIPITATION (CHEMISTRY); PROTEIN; ULTRAFILTRATION; WASTE TREATMENT; WASTEWATER;

ACTIVATED SLUDGE; ARTICLE; BIOFOULING; BIOMASS; CELL AGGREGATION; CONCENTRATION (PARAMETERS); CORRELATION ANALYSIS; DIALYSIS MEMBRANE; EXTRACELLULAR MATRIX; FREEZE DRYING; HOLLOW FIBER MEMBRANE; HYDROPHOBICITY; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MEMBRANE REACTOR; MOLECULAR WEIGHT; POLYACRYLAMIDE GEL ELECTROPHORESIS; POWDER; PROTEIN ANALYSIS; ULTRAFILTRATION; WASTE WATER MANAGEMENT;

DIALYSIS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FRACTIONAL PRECIPITATION; FREEZE DRYING; PROTEINS; PROTEOME; SEWAGE; ULTRAFILTRATION; WATER MICROBIOLOGY; WATER PURIFICATION;

EID: 84864590925     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4157-2     Document Type: Article

References (28)

1. Badireddy AR, Chellam S, Gassman PL, Engelhard MH, Lea AS, Rosso KM (2010) Role of extracellular polymeric substances in bioflocculation of activated sludge microorganisms under glucose-controlled conditions. Water Res 44(15):4505-4516
2. Bala Subramanian S, Yan S, Tyagi RD, Surampalli RY (2010) Extracellular polymeric substances (EPS) producing bacterial strains of municipal wastewater sludge: isolation, molecular identification, EPS characterization and performance for sludge settling and dewatering. Water Res 44(7):2253-2266
3. Benndorf D, Vogt C, Jehmlich N, Schmidt Y, Thomas H, Woffendin G, Shevchenko A, Richnow H-H, von Bergen M (2009) Improving protein extraction and separation methods for investigating the metaproteome of anaerobic benzene communities within sediments. Biodegradation 20(6):737-750
4. Buffle J, Leppard GG (1995) Characterization of aquatic colloids and macromolecules. 2. Key role of physical structures on analytical results. Environ Sci Technol 29(9):2176-2184
5. Comte S, Guibaud G, Baudu M (2006) Relations between extraction protocols for activated sludge extracellular polymeric substances (EPS) and EPS complexation properties: part I. Comparison of the efficiency of eight EPS extraction methods. Enzyme Microb Technol 38(1-2):237-245
6. D'Abzac P, Bordas F, Van Hullebusch E, Lens PNL, Guibaud G (2010) Extraction of extracellular polymeric substances (EPS) from anaerobic granular sludges: comparison of chemical and physical extraction protocols. Appl Microbiol Biotechnol 85(5):1589-1599
7. Denecke M (2006) Protein extraction from activated sludge. Water Sci Technol 54(1):175-181
8. Drews A (2010) Membrane fouling in membrane bioreactors-characterisation, contradictions, cause and cures. J Membr Sci 363(1-2):1-28
9. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28(3):350-356
10. Englard S, Seifter S (1990) Precipitation techniques. Methods Enzymol 182:285-300
11. Evans DRH, Romero JK, Westoby M (2009) Concentration of proteins and removal of solutes. In: Burgess RR, Deutscher MP (eds) Guide to protein purification, 2nd ed. Methods in Enzymology 463 pp 97-120
12. Flemming HC, Wingender J (2010) The biofilm matrix. Nat Rev Microbiol 8(9):623-633
13. Flemming HC, Neu TR, Wozniak DJ (2007) The EPS matrix: the "House of Biofilm cells". J Bacteriol 189(22):7945-7947
14. Freitas F, Alves VD, Carvalheira M, Costa N, Oliveira R, Reis MAM (2009) Emulsifying behaviour and rheological properties of the extracellular polysaccharide produced by Pseudomonas oleovorans grown on glycerol byproduct. Carbohydr Polym 78(3):549-556
15. Frolund B, Palmgren R, Keiding K, Nielsen PH (1996) Extraction of extracellular polymers from activated sludge using a cation exchange resin. Water Res 30(8):1749-1758
16. Ghasemi A, Hedayati M, Biabani H (2007) Protein precipitation methods evaluated for determination of serum nitric oxide end products by the Griess assay. J Med Sci Res 2:229-232
17. Higgins MJ, Novak JT (1997) Characterization of exocellular protein and its role in bioflocculation. J Environ Eng ASCE 123(5):479-485
18. Kuhn R, Pollice A, Laea G, Palese L, Lippolis R, Papa S (2007) Standard assays and metaproteomes as new approaches for functional characterization of membrane bioreactor biomass. In: Lesjean B (ed) Membrane technologies for wastewater treatment and reuse. KompetenzZentrum Wasser Berlin Publication, Berlin, pp 59-66
19. Liu H, Fang HHP (2002) Extraction of extracellular polymeric substances (EPS) of sludges. J Biotechnol 95(3):249-256
20. Park C, Novak JT, Helm RF, Ahn Y-O, Esen A (2008) Evaluation of the extracellular proteins in full-scale activated sludges. Water Res 42(14):3879-3889
21. Santos R, da Costa G, Franco C, Gomes-Alves P, Flammang P, Coelho AV (2009) First insights into the biochemistry of tube foot adhesive from the sea urchin Paracentrotus lividus (Echinoidea, Echinodermata). Mar Biotechnol (NY) 11(6):686-698
22. Seviour T, Donose BC, Pijuan M, Yuan Z (2010) Purification and conformational analysis of a key exopolysaccharide component of mixed culture aerobic sludge granules. Environ Sci Technol 44 (12):4729-4734
23. Sheng GP, Yu HQ, Yue ZB (2005) Production of extracellular polymeric substances from Rhodopseudomonas acidophila in the presence of toxic substances. Appl Microbiol Biotechnol 69 (2):216-222
24. Sheng GP, Yu HQ, Li XY (2010) Extracellular polymeric substances (EPS) of microbial aggregates in biological wastewater treatment systems: a review. Biotechnol Adv 28(6):882-894
25. Taylor EB, Williams MA (2010) Microbial protein in soil: influence of extraction method and C amendment on extraction and recovery. Microb Ecol 59(2):390-399
26. Wang ZW, Wu ZC, Tang SJ (2010) Impact of temperature seasonal change on sludge characteristics and membrane fouling in a submerged membrane bioreactor. Sep Sci Technol 45(7):920-927
27. Wilmes P, Bond PL (2006) Towards exposure of elusive metabolic mixed-culture processes: the application of metaproteomic analyses to activated sludge. Water Sci Technol 54(1):217-226
28. Wingender J, Neu T, Flemming H-C (1999) Microbial extracellular polymeric substances-characterization, structure and function. Springer, Berlin