Evaluation of thymus morphology and serum cortisol concentration as indirect biomarkers to detect low-dose dexamethasone illegal treatment in beef cattle

BMC Veterinary Research

Volumn 8, Issue , 2012, Pages

  Vascellari, Marta ^a   Katia, Capello ^a   Annalisa, Stefani ^a   Giancarlo, Biancotto ^a   Letizia, Moro ^a   Roberto, Stella ^a   Giandomenico, Pozza ^a   Franco, Mutinelli ^a  

^a ISTITUTO ZOOPROFILATTICO SPERIMENTALE DELLE VENEZIE   (Italy)

Author keywords

Beef cattle; Cortex medulla ratio; Cortisol; Dexamethasone; Indirect biomarker; Thymus

Indexed keywords

ANIMALIA; BOS;

BIOLOGICAL MARKER; DEXAMETHASONE; GROWTH PROMOTOR; HYDROCORTISONE;

ANIMAL; ANIMAL DISEASE; ARTICLE; BLOOD; CATTLE; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; DOSE RESPONSE; DRUG EFFECT; METHODOLOGY; PATHOLOGY; SENSITIVITY AND SPECIFICITY; SUBSTANCE ABUSE; THYMUS;

ANIMALS; BIOLOGICAL MARKERS; CATTLE; DEXAMETHASONE; DOSE-RESPONSE RELATIONSHIP, DRUG; GROWTH SUBSTANCES; HYDROCORTISONE; SENSITIVITY AND SPECIFICITY; SUBSTANCE ABUSE DETECTION; THYMUS GLAND;

EID: 84864526809     PISSN: None     EISSN: 17466148     Source Type: Journal    
DOI: 10.1186/1746-6148-8-129     Document Type: Article

References (34)

1. Renaville R, Massart S, Lognay G, Devolder A, Sneyers M, Marlier M, Severing M, Burny A, Portetelle D. Influence of a hormonal preparation containing glucocorticoids (dexamethasone esters), progestagen (chlormadinone acetate) and oestrogen (ethinyl oestradiol) on testosterone, insulin-like growth factor-1 (IGF-1), IGF-binding proteins and spermatogenic cells in finishing bulls. Anim Product 1994, 59:189-196.
2. Gottardo F, Brscic M, Pozza G, Ossensi C, Costiero B, Marin A, Cozzi G. Administration of dexamethasone per os in finishing bulls. I. Effects on productive traits, meat quality and cattle behaviour as indicator of welfare. Animal 2008, 2:1073-1079.
3. Meyer HH. Biochemistry and physiology of anabolic hormones used for improvement of meat production. Acta Pathol Microbiol Immunol Scandinavia 2001, 109:1-8.
4. Council Directive 96/23/EC of 29 April 1996 on measures to monitor certain substances and residues thereof in live animals and animal products and repealing Directives 85/358/EEC and 86/469/EEC and Decisions 89/187/EEC and 91/664/EEC. OJ EC L 125:10-31, 23.5 1996, , .
5. Commission Regulation (EU) N. 37/2010 of 22 December 2009 on pharmacologically active substances and their classification regarding maximum residue limits in foodstuffs of animal origin. OJ EC L 15:1-72, 20.1.2010 2010, , .
6. Courtheyn D, Le Bizec B, Brambilla G, De Brabander HF, Cobbaert E, Van De Wiele Vercammen J, De Wasch K. Recent developments in the use and abuse of growth promoters. Anal Chim Acta 2002, 473:71-82.
7. Biolatti B, Bollo E, Cannizzo FT, Zancanaro G, Tarantola M, Dacasto M, Cantiello M, Carletti M, Biolatti PG, Barbarino G. Effects of low-dose dexamethasone on thymus morphology and immunological parameters in veal calves. J Vet Med A 2005, 52:202-208.
8. Gardini G, Del Boccio P, Colombatto S, Testore G, Corpillo D, Di Ilio C, Urabani A, Nebbia C. Proteomic investigation in the detection of the illicit treatment of calves with growth-promoting agents. Proteomics 2006, 6:2813-2822. 10.1002/pmic.200500508, 16572471.
9. Toffolatti L, Rosa Gastaldo L, Patarnello T, Romualdi C, Merlanti R, Montesissa C, Poppi L, Castagnaro M, Bargelloni L. Expression analysis of androgen-responsive genes in the prostate of veal calves treated with anabolic hormones. Domest Anim Endocrinol 2006, 30:38-55. 10.1016/j.domaniend.2005.05.008, 16023321.
10. Reiter M, Walf VM, Christians A, Pfaffl MW, Meyer HH. Modification of mRNA expression after treatment with anabolic agents and the usefulness for gene expression-biomarkers. Anal Chim Acta 2007, 586:73-81. 10.1016/j.aca.2006.10.049, 17386698.
11. Courant F, Pinel G, Bichon E, Monteau F, Antignac JP, Le Bizec B. Development of a metabolomic approach based on liquid chromatography-high resolution mass spectrometry to screen for clenbuterol abuse in calves. Analyst 2009, 134:1637-1646. 10.1039/b901813a, 20448932.
12. Stella R, Biancotto G, Krogh M, Angeletti R, Pozza G, Sorgato MC, James P, Andrighetto I. Protein expression changes in skeletal muscle in response to growth promoter abuse in beef cattle. J Proteome Res 2011, 10:2744-2757. 10.1021/pr101255c, 21425879.
13. Relazione finale. Piano Nazionale Residui 2009 2009, 1-63. , Roma, Italy., http://www.salute.gov.it
14. Vascellari M, Pozza G, Poppi L, Capello K, Angeletti R, Ravarotto L, Andrighetto I, Mutinelli F. Evaluation of indirect biomarkers of corticosteroids use as illegal growth promoters in beef cattle. Vet Rec 2008, 163:147-152. 10.1136/vr.163.5.147, 18676998.
15. Cannizzo FT, Capra P, Divari S, Ciccotelli V, Biolatti B, Vincenti M. Effects of low-dose dexamethasone and prednisolone long term administration in beef calf: chemical and morphological investigation. Anal Chim Acta 2011, 700:95-104. 10.1016/j.aca.2010.12.004, 21742122.
16. Council Directive Council Directive 86/609/EEC of 24 November 1986 on the approximation of laws, regulations and administrative provisions of the Member States regarding the protection of animals used for experimental and other scientific purposes. OJ EEC L 358:1-28, 18.12.1986 1986, , .
17. Decreto Legislativo 27-1-1992 n. 116. Attuazione della direttiva n. 86/609/CEE in materia di protezione degli animali utilizzati a fini sperimentali o ad altri fini scientifici. GURI 18 febbraio 1992, n. 40, S.O. 1992, , .
18. Commission Decision of 12 August 2002 implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of results (2002/657/EC). OJ EC L 221:8-36, 17.8.2002 2002, , .
19. Bozzetta E, Pezzolato M, Maurella C, Varello K, Richelmi GB, Draisci R, Ferranti C, D'Angelo A, Caramelli M. Development of an enhanced histopathological approach to detect low-dose dexamethasone illicit treatment in veal calves. Food Additives and Contaminants A 2011, 28:1187-1192.
20. Groot MJ, Schilt R, Ossenkoppele JS, Berende PL, Haasnoot W. Combinations of growth promoters in veal calves: consequences for screening and confirmation methods. Zentralblatt für Veterinärmedizin A 2008, 45:425-440.
21. Vincenti M, Girolami F, Capra P, Pazzi M, Carletti M, Gardini G, Nebbia C. Study of dexamethasone urinary excretion profile in cattle by LC-MS/MS: comparison between theraputic and growth-promoting administration. J Agric Food Chem 2009, 57:1299-1306. 10.1021/jf803465d, 19182943.
22. Vanhaecke L, Antignac JP, Courtheyn D, Le Bizec B, De Brabander H. Elimination kinetics of dexamethasone in bovine urine, hair and feces following single administration of dexamethasone acetate and phosphate esters. Steroids 2011, 76:111-117. 10.1016/j.steroids.2010.09.007, 20888849.
23. Cannizzo FT, Miniscalco B, Riondato F, Bollo E, Barbarino G, Giorgi P, Mazzini C, Biolatti B. Effects of anabolic and therapeutic doses of dexamethasone on thymus morphology and apoptosis in veal calves. Vet Rec 2008, 163:448-452. 10.1136/vr.163.15.448, 18849577.
24. Gruver AL, Sempowski GD. Cytokines, leptin, and stress-induced thymic atrophy. J Leukoc Biol 2008, 84:915-923. 10.1189/jlb.0108025, 2538595, 18495786.
25. Cannizzo FT, Spada F, Benevelli R, Nebbia C, Giorgi P, Brina N, Bollo E, Biolatti B. Thymus atrophy and regeneration following dexamethasone administration to beef cattle. Vet Rec 2010, 167:338-343. 10.1136/vr.c3303, 20802188.
26. Wyllie AH. Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature 1980, 284:555-556. 10.1038/284555a0, 6245367.
27. Zubkova I, Mostowski H, Zaitseva M. Up-regulation of IL-7, stromal-derived factor-1 alpha, thymus-expressed chemokine, and secondary lymphoid tissue chemokine gene expression in the stromal cells in response to thymocyte depletion: implication for thymus reconstitution. J Immunol 2005, 175:2321-2330.
28. Wang SD, Huang KJ, Lin YS, Lei HY. Sepsis-induced apoptosis of the thymocytes in mice. J Immunol 1994, 152:5014-5021.
29. Valli VEO. Hematopoietic system. Jubb, Kennedy, and Palmer's Pathology of domestic animals 2007, 107-324. Elsevier, Amsterdam, Maxie MG, 5.
30. Grandin T. Assessment of stress during handling and transport. J Anim Sci 1997, 75:249-257.
31. Odore R, D'angelo A, Badino P, Bellino C, Pagliasso S, Re G. Road transportation affects blood hormone levels and lymphocyte glucocorticoid and β adrenergic receptor concentrations in calves. Vet J 2004, 168:297-303. 10.1016/j.tvjl.2003.09.008, 15501147.
32. Meij BP, Mol JA. Adrenocortical function. Clinical biochemistry of domestic animals 2008, 605-622. Academic, San Diego, Kaneko JJ, Harvey JW, Bruss ML, 6.
33. Abraham G, Allersmeier M, Gottschalk J, Schusser GF, Hoppen HO, Ungemach FR. Effects of dermal dexamethasone application on ACTH and both basal and ACTH-stimulated cortisol concentration in normal horses. J Vet Pharmacol Theriol 2009, 32:379-387.
34. Marin A, Pozza G, Gottardo F, Moro L, Stefani AL, Cozzi G, Brscic M, Andrighetto I, Ravarotto L. Administration of dexamethasone per os in finishing bulls: II. Effects on blood parameters used as indicators of animal welfare. Animal 2008, 2:1080-1086.