Improved real-time PCR estimation of gene copy number in soil extracts using an artificial reference

Journal of Microbiological Methods

Volumn 91, Issue 1, 2012, Pages 38-44

  Daniell, T J ^a   Davidson, J ^a   Alexander, C J ^b   Caul, S ^a   Roberts, D M ^a  

^a SCOTTISH CROP RESEARCH INSTITUTE   (United Kingdom)

^b BIOMATHEMATICS AND STATISTICS SCOTLAND   (United Kingdom)

Author keywords

Gene copy quantification; Internal standard; Quantitative PCR; Relative estimation; Soil ecology

Indexed keywords

ESTER LINKED FATTY ACID; FATTY ACID; UNCLASSIFIED DRUG;

ACCURACY; ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; DNA EXTRACTION; DNA TEMPLATE; ESCHERICHIA COLI; FATTY ACID ANALYSIS; GENE DOSAGE; HOUSEKEEPING GENE; POPULATION SIZE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SOIL MICROFLORA; STANDARD;

DNA, BACTERIAL; ESCHERICHIA COLI; GENE DOSAGE; REAL-TIME POLYMERASE CHAIN REACTION; REFERENCE STANDARDS; SOIL MICROBIOLOGY;

BACTERIA (MICROORGANISMS);

EID: 84864518655     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2012.07.010     Document Type: Article

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