Rapid detection of viable M. Tuberculosis directly from sputum by reverse transcriptase PCR targeting 85B gene

Journal of Communicable Diseases

Volumn 43, Issue 2, 2011, Pages 89-96

  Gayathri, Ramasubban ^a   Therese, Kulandai Lily ^a,b   Sridhar, R ^b   Kannan, Durai ^b   Madhavan, Hajib N ^a   Meenakshi, N ^b  

^a VISION RESEARCH FOUNDATION   (India)

^b Institute of Thoracic Medicine   (India)

Author keywords

M tuberculosis; RT PCR; Viable

Indexed keywords

BACTERIAL ANTIGEN;

ARTICLE; HUMAN; ISOLATION AND PURIFICATION; LUNG TUBERCULOSIS; METHODOLOGY; MICROBIOLOGY; MYCOBACTERIUM TUBERCULOSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPUTUM;

ANTIGENS, BACTERIAL; HUMANS; MYCOBACTERIUM TUBERCULOSIS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPUTUM; TUBERCULOSIS, PULMONARY;

EID: 84864473862     PISSN: 00195138     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (15)

1. Global tuberculosis control-surveillance, planning, financing. WHO Report 2008 WHO/HTM/TB/2008. 393.
2. Yoneda, M., Fukui, Y. and Yamanouchi T. Extracellular proteins of tubercle bacilli. V. Distribution of A and B antigens in various mycobacteria. Biken J 1965; 8: 201-203.
3. Wilker, H.G., Harboe, M. and Lea, T. Purification and characterization of two protein antigens from heerogenous BCG 85B complex in Mycobacterium bovis BCG. Int. Arch. Allergy Appl. Immunol 1986; 81: 298-306. (Pubitemid 17176033)
4. Salman H. Siddiqui. BACTEC 460TB Syatem, Product and Procedure Manual MA0029, Becton Dickinson and Company, Maryland, USA.
5. Desjardin, L.E., Perkins, M.D., Teixeira, L., et al. Alkaline decontamination of sputum specimens adversely affects stability of Mycobacterial mRNA. 1996. J. Clin. Microbiol 1996; 34: 2435-2439. (Pubitemid 26307787)
6. High capacity cDNA conversion kit Archive. Applied Biosystems. 2000.
7. Jou, N.T., Yoshimori, R. B., Mason, G. R., et al. Single-tube, nested, reverse transcriptase PCR for detection of viable Mycobacterium tuberculosis. J Clin. Microbio. 1997; 35: 1161-1165. (Pubitemid 27175244)
8. Gunisha P, Madhavan HN, Therese KL, et al. Polymerase chain reaction usmg IS6110 primer to detect Mycobacterium tuberculosis in clinical samples. Indian J Path Microbiol 2000; 43: 395-402.
9. Negi, S.S., Pasha, S.T., Gupta, S., et al. Diagnostic potential of IS6110, 38KDA, 65 KDA and 85B sequence based Polymerase chain reaction in the diagnosis of Mycobacterium tuberculosis in clinical samples. Ind J Med Microbiol 2007; 25: 43-49. (Pubitemid 46466020)
10. Therese KL, Jayanthi U, Madhavan HN. Application of nested Polymerase Chain Reaction CnPCR) using MPB64 gene primers to detect Mycobacterium tuberculosis DNA in clinical specimens from extrapulmonary tuberculosis patients. Indian J Med Res 2005; 122: 165-170. (Pubitemid 41404140)
11. Biswas J, Krishnakumar S, Therese KL, et al. Detection of Mycobacterium tuberculosis by Nested Polymerase chain reaction in a case of sub-conjunctival tuberculosis. Cornea 2002; 21: 123-125. (Pubitemid 33144419)
12. Biswas J, Therese KL, Madhavan HN, et al. Detection of Mycobacterium tuberculosis by polymerase chain reaction in a case of orbital tuberculosis. Orbital Lesions 2001; 22: 69-74. (Pubitemid 32656025)
13. Madhavan HN, Therese KL, Gunisha P, et al. Polymerase chain reaction for detection of Mycobacterium tuberculosis in epiretinal membrane in Eales' disease. Invest Ophthalmol Vis Sci 2000; 41: 822-825. (Pubitemid 30158894)
14. Biswas J, Therese KL and Madhavan HN. Use of polymerase chain reaction in detection of Mycobacterium tuberculosis complex DNA from vitreous sample of Eales' disease. Br J Ophthalmol 1999; 83: 994. (Pubitemid 29376046)
15. Desjardin LE, Perkins MD, Wolski K, et al. Measurement of Sputum Mycobacterium tuberculosis Messenger RNA as a surrogate for response to chemotherapy. Am J Respir Crit Care Med 1999; 160: 203-210. (Pubitemid 29326089)