Cloning, expression and characterization of meso-2,3-butanediol dehydrogenase from Klebsiella pneumoniae

Biotechnology Letters

Volumn 34, Issue 8, 2012, Pages 1519-1523

  Zhang, Gen Lin ^a,b   Wang, Cui Wei ^a   Li, Chun ^a  

^a BEIJING INSTITUTE OF TECHNOLOGY   (China)

^b SHIHEZI UNIVERSITY   (China)

Author keywords

2,3 Butanediol dehydrogenase; Enzymatic properties; Expression; Klebsiella pneumoniae; Substrate specificity

Indexed keywords

2 ,3-BUTANEDIOL; ENZYMATIC PROPERTIES; EXPRESSION; KLEBSIELLA PNEUMONIAE; SUBSTRATE SPECIFICITY;

AMINO ACIDS; ENZYMES; ESCHERICHIA COLI; GENETIC ENGINEERING;

GENE ENCODING;

2,3 BUTYLENE GLYCOL; 2,3-BUTYLENE GLYCOL; ALCOHOL DEHYDROGENASE; BACTERIAL PROTEIN; BUTANEDIOL; BUTANEDIOL DEHYDROGENASE; RECOMBINANT PROTEIN;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; KLEBSIELLA PNEUMONIAE; METABOLISM; MOLECULAR CLONING; OXIDATION REDUCTION REACTION; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; TEMPERATURE;

ALCOHOL OXIDOREDUCTASES; BACTERIAL PROTEINS; BUTYLENE GLYCOLS; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; KLEBSIELLA PNEUMONIAE; OXIDATION-REDUCTION; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; TEMPERATURE;

ESCHERICHIA COLI; KLEBSIELLA PNEUMONIAE;

EID: 84864394036     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-012-0933-4     Document Type: Article

References (15)

1. Celińska E, Grajek W (2009) Biotechnological production of 2, 3-butanediol-current state and prospects. Biotechnol Adv 27: 715-725.
2. Converti A, Perego P, Del Borghi M (2003) Effect of specific oxygen uptake rate on Enterobacter aerogenes energetics: carbon and reduction degree balances in batch cultivations. Biotechnol Bioeng 82: 370-733.
3. González E, Fernández MR, Larroy C et al (2000) Characterization of a (2R, 3R)-2, 3-butanediol dehydrogenase as the Saccharomyces cerevisiae YAL060W gene product. J Biol Chem 275(46): 35876-35885.
4. González E, Fernández MR, Larroy C et al (2001) Characterization and functional role of Saccharomyces cerevisiae 2, 3-butanediol dehydrogenase. Chem Biol Interact 130-132: 425-434.
5. Ji XJ, Huang H, Li S et al (2008) Enhanced 2, 3-butanediol production by altering the mixed acid fermentation pathway in Klebsiella oxytoca. Biotechnol Lett 30: 731-734.
6. Ma C, Wang A, Qin J et al (2009) Enhanced 2, 3-butanediol production by Klebsiella pneumoniae SDM. Appl Microbiol Biotechnol 82: 49-57.
7. Nicholson WL (2008) The Bacillus subtilis ydjL (bdhA) gene encodes acetoin reductase/2, 3-butanediol dehydrogenase. Appl Environ Microbiol 74(22): 6832-68388.
8. Ui S, Okajima Y, Mimura A et al (1997a) Molecular generation of an Escherichia coli strain producing only the meso-isomer of 2, 3-butanediol. J Ferment Bioeng 84(3): 185-189.
9. Ui S, Okajima Y, Mimura A et al (1997b) Sequence analysis of the gene for and characterization of D-acetoin forming meso-2, 3-butanediol dehydrogenase of Klebsiella pneumoniae expressed in Escherichia coli. J Ferment Bioeng 83(1): 32-37.
10. Ui S, Mimura A, Ohkuma M, Kudo T (1999) Formation of chiral acetoinic compound from diacetyl by Escherichia coli expressing meso-2, 3-butanediol dehydrogenase. Lett Appl Microbiol 28: 457-460.
11. Ui S, Takusagawa Y, Sato T et al (2004) Production of L-2, 3-butanediol by a new pathway constructed in Escherichia coli. Lett Appl Microbiol 39: 533-537.
12. van Houdt R, Aertsen A, Michiels CW (2007) Quorum-sensing-dependent switch to butanediol fermentation prevents lethal medium acidification in Aeromonas hydrophila AH-1 N. Res Microbiol 158: 379-385.
13. Wardwell SA, Yang YT, Chang HY et al (2001) Expression of the Klebsiella pneumoniaeCG21 acetoin reductase gene in Clostridium acetobutylicum ATCC 824. J Ind Microbiol Biotechnol 27: 220-227.
14. Yin SM, Zheng Y, Tien P (2003) On-column purification and refolding of recombinant bovine prion protein: using its octarepeat sequences as a natural affinity tag. Protein Expres Purif 32: 104-109.
15. Zhang GL, Jiang YL, Ban LL (2011) Process optimization for producing 2, 3-butanediol by fermentation of cotton stalk hydrolysate after detoxification. Trans CSAE 27(5): 287-291 (in Chinese with English abstract).