Development of a SYBR Green I-based real-time PCR for detection of elephant endotheliotropic herpesvirus 1 infection in Asian elephants (Elephas maximus)

Journal of Virological Methods

Volumn 185, Issue 1, 2012, Pages 160-165

  Sariya, Ladawan ^a   Chatsirivech, Jarin ^a   Suksai, Parut ^a   Wiriyarat, Witthawat ^a   Songjaeng, Adisak ^b   Tangsudjai, Siriporn ^a   Kanthasaewee, Oraphan ^a   Maikaew, Umaporn ^c   Chaichoun, Kridsada ^a  

^a MAHIDOL UNIVERSITY   (Thailand)

^b MAHIDOL UNIVERSITY   (Thailand)

^c Zoological Park Organization Bangkok   (Thailand)

Author keywords

EEHV1; Elephant endotheliotropic herpesvirus 1; Real time SYBR Green I based PCR

Indexed keywords

FLUORESCENT DYE; NUCLEOTIDE; SYBR GREEN I; UNCLASSIFIED DRUG; VIRUS DNA;

ANALYTICAL EQUIPMENT; ANIMAL TISSUE; ARTICLE; ASIA; AUTOPSY; BETAHERPESVIRINAE; CONTROLLED STUDY; CROSS REACTION; ELEPHANT; ELEPHANT ENDOTHELIOTROPIC HERPESVIRUS 1; ELEPHANT ENDOTHELIOTROPIC HERPESVIRUS 1 INFECTION; ELEPHANT ENDOTHELIOTROPIC HERPESVIRUS 1A; EQUINE HERPES VIRUS; EQUINE HERPES VIRUS 2; HEART; HERPES SIMPLEX VIRUS 1; HERPES VIRUS INFECTION; LIMIT OF DETECTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SPLEEN; TERMINASE GENE; TRUNK; VIRUS CLASSIFICATION; VIRUS GENE; VIRUS IDENTIFICATION; VIRUS LOAD;

ANIMAL STRUCTURES; ANIMALS; DNA, VIRAL; ELEPHANTS; HERPESVIRIDAE; HERPESVIRIDAE INFECTIONS; MOLECULAR DIAGNOSTIC TECHNIQUES; MOLECULAR SEQUENCE DATA; ORGANIC CHEMICALS; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA; STAINING AND LABELING; VETERINARY MEDICINE; VIROLOGY;

DNA VIRUSES; ELEPHAS MAXIMUS; EQUID HERPESVIRUS 2; HUMAN HERPESVIRUS 1; MAMMALIA;

EID: 84864312058     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2012.06.005     Document Type: Article

References (14)

1. Davison A.J., Eberle R., Ehlers B., Hayward G.S., McGeoch D.J., Minson A.C., Pellett P.E., Roizman B., Studdert M.J., Thiry E. The order herpesvirales. Archives of Virology 2009, 154:171-177.
2. Ehlers B., Dural G., Marschal l M., Schregel V., Goltz1 M., Hentschke J. Endotheliotropic elephant herpesvirus, the first betaherpesvirus with a thymidine kinase gene. Journal of General Virology 2006, 87:2781-2789.
3. Fickel J., Richman L.K., Montali R., Schaftenaar W., Goritz F., Hildebrandt T.B., Pitra C. A variant of the endotheliotropic herpesvirus in Asian elephants (Elephas maximus) in European zoos. Veterinary Microbiology 2001, 82:103-109.
4. Fickel J., Lieckfeldt D., Richman L.K., Streich W.J., Hildebrandt T.B., Pitra C. Comparison of glycoprotein B (gB) variants of the elephant endotheliotropic herpesvirus (EEHV) isolated from Asian elephants (Elephas maximus). Veterinary Microbiology 2003, 91:11-21.
5. Garner M.M., Helmick K., Ochsenreiter J., Richman L.K., Latimer E., Wise A.G., Maes R.K., Kiupel M., Nordhausen R.W., Zong J.C., Hayward G.S. Clinico-pathologic features of fatal disease attributed to new variants of endotheliotropic herpesviruses in two Asian elephants (Elephas maximus). Veterinary Pathology 2009, 46:97-104.
6. Hardman K., Dastjerdi A., Gurrala R., Routh A., Banks M., Steinbach F., Bouts T. Detection of elephant endotheliotropic herpesvirus type 1 in asymptomatic elephants using TaqMan real-time PCR. Veterinary Record 2011, 170(8):205.
7. Latimer E., Zong J.C., Heaggans S.Y., Richman L.K., Hayward G.S. Detection and evaluation of novel herpesviruses in routine and pathological samples from Asian and African elephants: identification of two new probosciviruses (EEHV5 and EEHV6) and two new gammaherpesviruses (EGHV3B and EGHV5). Veterinary Microbiology 2011, 147:28-41.
8. Ossent P., Guscetti F., Metzler A.E., Lang E.M., Rubel A., Hauser B. Acute and fetal herpesvirus infection in young Asian elephant (Elephas maximus). Veterinary Pathology 1990, 7:131-133.
9. Reid C., Hildebrandt T., Marx N., Hunt M., Thy N., Reynes J., Schaftenaar W., Fickel J. Endotheliotropic elephant herpes virus (EEHV) infection. The first PCR-confirmed fatal case in Asia. Veterinary Quarterly 2006, 28:61-64.
10. Richman L.K., Montali R.J., Garber R.L., Kennedy M.A., Lehnhatdt J., Hildebrandt T., Schmitt D., Hardy D., Alcendor D.J., Hayward G.J. Novel endotheliotropic herpesviruses fatal for Asian and African elephant. Science 1999, 283:1171-1176.
11. Richman L.K., Montali R.J., Cambre R.C., Schmitt D., Hardy D., Hildbrandt T., Bengis R.G., Hamzeh F.M., Shahkolahi A., Hayward G.S. Clinical and pathological findings of a newly recognized disease of elephants caused by endotheliotropic herpesviruses. Journal of Wildlife Diseases 2000, 36:1-12.
12. Ryan S.J., Thompson S.D. Disease risk and inter-institutional transfer of specimens in cooperative breeding programs Herpes and the elephant species survival plan. Zeitschrift fur Biologie 2001, 20:89-101.
13. Schaftenaar W., Reid C., Martina B., Fickel J., Osterhaus A.D. Nonfatal clinical presentation of elephant endotheliotropic herpes virus discovered in a group of captive Asian elephants (Elephas maximus). Journal of Zoo and Wildlife Medicine 2010, 41(4):626-632.
14. Stanton J.J., Zong J.C., Latimer E., Tan J., Herron A., Hayward G.S., Ling P.D. Detection of pathogenic elephant endotheliotropic herpesvirus in routine trunk washes from healthy adult Asian elephants (Elephas maximus) by use of a real-time quantitative polymerase chain reaction assay. American Journal of Veterinary Research 2010, 71:925-933.