A simple method for the enumeration of methanogens by most probable number counting

Biomass and Bioenergy

Volumn 45, Issue , 2012, Pages 311-314

  Wagner, Andreas Otto ^a   Lins, Philipp ^a   Illmer, Paul ^a  

^a UNIVERSITY OF INNSBRUCK   (Austria)

Author keywords

Biogas; Digester sludge; Methanogens; Most probable number; MPN; Soil

Indexed keywords

ARCHAEA; CULTURE-BASED METHODS; DIGESTER SLUDGE; METABOLIC PRODUCTS; MOST PROBABLE NUMBER; MPN; SIMPLE METHOD;

BIOGAS; METHANE; SOILS;

METHANOGENS;

BIOGAS; ESTIMATION METHOD; METHANE; METHANOGENESIS; SLUDGE; SOIL EMISSION;

ARCHAEA;

EID: 84864302312     PISSN: 09619534     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biombioe.2012.06.015     Document Type: Article

References (19)

1. Frostegard A., Courtois S., Ramisse V., Clerc S., Bernillon D., LeGall F., et al. Quantification of bias related to the extraction of DNA directly from soils. Appl Environ Microbiol 1999, 65(12):5409-5420.
2. LaMontagne M.G., Michel F.C., Holden P.A., Reddy C.A. Evaluation of extraction and purification methods for obtaining PCR-amplifiable DNA from compost for microbial community analysis. J Microbiol Methods 2002, 49(3):255-264.
3. Miller D.N., Bryant J.E., Madsen E.L., Ghiorse W.C. Evaluation and optimization of DNA extraction and purification procedures for soil and sediment samples. Appl Environ Microbiol 1999, 65(11):4715-4724.
4. Speksnijder A.G.C.L., Kowalchuk G.A., DeJong S., Kline E., Stephen J.R., Laanbroek H.J. Microvariation artifacts introduced by PCR and cloning of closely related 16S rRNA gene sequences. Appl Environ Microbiol 2001, 67(1):469-472.
5. Yang Z.H., Xiao Y., Zeng G.M., Xu Z.Y., Liu Y.S. Comparison of methods for total community DNA extraction and purification from compost. Appl Microbiol Biotechnol 2007, 74(4):918-925.
6. Taya M., Aoki N., Kobayashi T. Kinetic evaluation and monitoring of methanogen culture based upon fluorometry. Appl Microbiol Biotechnol 1986, 23(5):342-347.
7. 420 in methanogenic sludge by the use of reversed-phase high-performance liquid chromatography and a fluorescence detector. Appl Microbiol Biotechnol 1983, 18(1):67-69.
8. Woomer P.L. Most probable number counts. Methods in soil analysis - part 2: microbiological and biochemical properties 1994, 59-79. Soil Science Society of America, Inc., Madison, USA. J.M. Bigham, S.H. Mickelson (Eds.).
9. Halvorson H.O., Ziegler N.R. Application of statistics to problems in bacteriology. I. A means of determining bacterial population by dilution method. J Bacteriol 1933, 25(2):101-121.
10. Cochran W.G. Estimation of bacterial densities by means of "most probable number" Biometrics 1950, 6:105-116.
11. Illmer P., Schwarzenauer T., Malin C., Wagner A.O., Miller L.M., Gstraunthaler G. Process parameters within a 750,000 litre anaerobic digester during a year of disturbed fermenter performance. Waste Manag 2009, 29(6):1838-1843.
12. Wagner A.O., Malin C., Gstraunthaler G., Illmer P. Survival of selected pathogens in diluted sludge of a thermophilic waste treatment plant and in NaCl-solution under aerobic and anaerobic conditions. Waste Manag 2009, 29(1):425-429.
13. Wagner AO, Hohlbrugger P, Lins P, Illmer P. Effects of different nitrogen sources on the biogas production - a lab-scale investigation. Microbiol Res, in press. doi:10.1016/j.micres.2011.11.007.
14. Schinner F., Öhlinger R., Kandeler E., Margesin R. Methods in soil biology 1996, Springer, Heidelberg.
15. 2 exchange and its components in mountain grasslands. Biogeosciences 2010, 7:2297-2309.
16. Wagner AO, Hofmann K, Prem E, Illmer P. Methanogenic activities in alpine soils. Folia Microbiol, in press. doi:10.1007/s12223-012-0145-2.
17. Wagner A.O., Gstraunthaler G., Illmer P. Utilisation of single added fatty acids by consortia of digester sludge in batch culture. Waste Manag 2010, 30(10):1822-1827.
18. Wagner A.O., Malin C., Lins P., Illmer P. Effects of various fatty acid amendments on a microbial digester community in batch culture. Waste Manag 2011, 31(3):431-437.
19. Blodgett R. Appendix 2 - most probable number from serial dilutions. Bacteriological Analytical Manual (BAM) 1998, Revision A. Available from:, last updated 10/2010 [cited 2012 May 28]. http://www.fda.gov/Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm109656.htm, 8th ed. T. Hammack, P. Feng, K. Jinneman, P.M. Regan, J. Kase, P. Orlandi, W. Burkhardt, BAM council (Eds.).