Tools for characterizing the whole-cell bio-oxidation of alkanes at microscale

Biotechnology and Bioengineering

Volumn 109, Issue 9, 2012, Pages 2179-2189

  Grant, Chris ^a   da Silva Damas Pinto, Ana Catarina ^a   Lui, Hai Po ^a   Woodley, John M ^b   Baganz, Frank ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

Design of experiments; Monooxygenase; Scale down; Substrate solubility; Two liquid phase; Whole cell biocatalysis

Indexed keywords

1-DODECANOL; ALKANE HYDROXYLASE; AQUEOUS PHASE; BIO-OXIDATION; BIO-OXIDATION SYSTEM; DODECANOIC ACID; E. COLI; GLUCOSE CONCENTRATION; IN-VIVO; LONG CHAIN ALKANE; MASS TRANSFER RATE; MICRO WELLS; MICRO-SCALES; MONOOXYGENASES; N-DODECANE; N-OCTANES; OVEROXIDATIONS; PURIFIED ENZYME; RESPONSE SURFACE METHODOLOGY; SCALE-DOWN; SPECIFIC ACTIVITY; SPECIFIC YIELD; STIRRED TANK BIOREACTORS; STIRRED TANK REACTORS; TWO-LIQUID PHASE; WHOLE CELL; WHOLE-CELL BIOCATALYSIS;

BIOLOGICAL WATER TREATMENT; CELLS; DESIGN OF EXPERIMENTS; ESCHERICHIA COLI; GLUCOSE; MICROCHANNELS; PARAFFINS; SATURATED FATTY ACIDS; SOLUBILITY;

CYTOLOGY;

ALKANE 1 MONOOXYGENASE; ALKANE DERIVATIVE; ALKANE HYDROXYLASE; LAURIC ACID; UNCLASSIFIED DRUG;

ARTICLE; BIOCATALYSIS; BIOTRANSFORMATION; CELL DENSITY; CHEMICAL ANALYSIS; CHEMICAL PROCEDURES; ESCHERICHIA COLI; HYDROXYLATION; NONHUMAN; OXIDATION; STIRRED REACTOR; TWO LIQUID PHASE MICROWELL SYSTEM;

ALDEHYDES; ALKANE 1-MONOOXYGENASE; ALKANES; BACTERIAL PROTEINS; BIOREACTORS; BIOTECHNOLOGY; DODECANOL; ESCHERICHIA COLI; FERMENTATION; OXIDATION-REDUCTION; OXYGEN; RESEARCH DESIGN; SOLUBILITY;

ESCHERICHIA COLI;

EID: 84864296107     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24512     Document Type: Article

References (20)

1. Cánovas M, Torroglosa T, Iborra JL. 2005. Permeabilization of Escherichia coli cells in the biotransformation of trimethylammonium compounds into l-carnitine. Enzyme Microb Technol 37(3): 300-308.
2. Doig SD, Pickering SCR, Lye GJ, Baganz F. 2005. Modelling surface aeration rates in shaken microtitre plates using dimensionless groups. Chem Eng Sci 60(10): 2741-2750.
3. Duetz WA. 2007. Microtiter plates as mini-bioreactors: Miniaturization of fermentation methods. Trends Microbiol 15(10): 469-475.
4. Eggink G, Lageveen RG, Altenburg B, Witholt B. 1987. Controlled and functional expression of the Pseudomonas oleovorans alkane utilizing system in Pseudomonas putida and Escherichia coli. J Biol Chem 262(36): 17712-17718.
5. Favre-Bulle O, Schouten T, Kingma J, Witholt B. 1991. Bioconversion of n-octane to octanoic acid by a recombinant Escherichia coli cultured in a two-liquid phase bioreactor. Biotechnology (NY) 9(4): 367-371.
6. Girhard M, Machida K, Itoh M, Schmid RD, Arisawa A, Urlacher VB. 2009. Regioselective biooxidation of (+)-valencene by recombinant E. coli expressing CYP109B1 from Bacillus subtilis in a two-liquid-phase system. Microb Cell Fact 8(1): 36-36.
7. Goswami P, Singh HD. 1991. Different modes of hydrocarbon uptake by twoPseudomonas species. Biotechnol Bioeng 37(1): 1-11.
8. Goswami P, Hazarika AK, Singh HD. 1994. Hydrocarbon pseudosolubilizing and emulsifying proteins produced by Pseudomonas cepacia N1. J Ferment Bioeng 77(1): 28-31.
9. Grant C, Woodley JM, Baganz F. 2011. Whole-cell bio-oxidation of n-dodecane using the alkane hydroxylase system of P. putida GPo1 expressed in E. coli. Enzyme Microb Technol 48(6-7): 480-486.
10. Helander IM, Alakomi H-L, Latva-Kala K, Koski P. 1997. Polyethyleneimine is an effective permeabilizer of Gram-negative bacteria. Microbiology 143(10): 3193-3199.
11. Lye GJ, Woodley J, Cabral JMS, Mota M, Tramper J. 2001. Chapter 5: Advances in the selection and design of two-liquid phase biocatalytic reactors. Multiphase bioreactor design. London, UK: Taylor & Francis Publishers. p 115-134.
12. Marques MPC, de Carvalho CCCR, Claudino MJC, Cabral J, Fernandes P. 2007. On the feasibility of the microscale approach for a multistep biotransformation: Sitosterol side chain cleavage. J Chem Technol Biotechnol 82(9): 856-863.
13. Marques MPC, Carvalho F, Magalhães S, Cabral J, Fernandes P. 2009. Screening for suitable solvents as substrate carriers for the microbial side-chain cleavage of sitosterol using microtitre plates. Process Biochem 44(5): 556-561.
14. Maurer SC, Schulze H, Schmid RD, Urlacher V. 2003. Immobilisation of P450 BM-3 and an NADP+ cofactor recycling system: Towards a technical application of heme-containing monooxygenases in fine chemical synthesis. Adv Synth Catalysis 345(6-7): 802-810.
15. Setti L, Pifferi PG, Lanzarini G. 1995. Surface tension as a limiting factor for aerobic n-alkane biodegradation. J Chem Technol Biotechnol 64(1): 41-48.
16. Shaw DG. 1989. Hydrocarbons with water and seawater. Oxford: Pergamon Press.
17. Van Beilen JB. 2004. Chapter 14: Alkane degradation by pseudomonads. Pseudomonas: Biosynthesis of macromolecules and molecular metabolism. New York, NY: Kluwer Academic/Plenum Publishers.
18. van Beilen JB, Wubbolts MG, Witholt B. 1994. Genetics of alkane oxidation by Pseudomonas oleovorans. Biodegradation 5(3-4): 161-174.
19. Van Sonsbeek HM, Beeftink HH, Tramper J. 1993. Two-liquid-phase bioreactors. Enzyme Microb Technol 15(9): 722-729.
20. Wubbolts M, Favre-Bulle O, Witholt B. 1996. Biosynthesis of synthons in two-liquid-phase media. Biotechnol Bioeng 52(2): 301-308.