DNA methyltransferases 1 and 3b expression in Huh-7 cells expressing HCV core protein of different genotypes

Digestive Diseases and Sciences

Volumn 57, Issue 6, 2012, Pages 1598-1603

  Benegiamo, Giorgia ^a   Vinciguerra, Manlio ^b   Mazzoccoli, Gianluigi ^a   Piepoli, Ada ^a   Andriulli, Angelo ^a   Pazienza, Valerio ^a  

^a CASA SOLLIEVO DELLA SOFFERENZA HOSPITAL   (Italy)

^b INSTITUTE OF HEPATOLOGY   (United Kingdom)

Author keywords

CDH1; DNMTs; HCV core; SIRT1; STAT3

Indexed keywords

CORE PROTEIN; DNA METHYLTRANSFERASE 1; DNA METHYLTRANSFERASE 3B; MESSENGER RNA; SIRTINOL; SIRTUIN 1; STAT3 PROTEIN; UVOMORULIN;

ARTICLE; CONTROLLED STUDY; ENZYME INDUCTION; GENE CONTROL; GENETIC TRANSFECTION; GENOTYPE; HEPATITIS C VIRUS; HUMAN; HUMAN CELL; IN VITRO STUDY; LIVER CARCINOGENESIS; LIVER CELL CARCINOMA; NUCLEOTIDE SEQUENCE; PATHOGENESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ANIMALS; BENZAMIDES; CADHERINS; CARCINOMA, HEPATOCELLULAR; CATTLE; CELL TRANSFORMATION, NEOPLASTIC; DNA (CYTOSINE-5-)-METHYLTRANSFERASE; DOWN-REGULATION; GENE EXPRESSION REGULATION; GENOTYPE; HEPACIVIRUS; HEPATITIS C, CHRONIC; HUMANS; IMMUNOBLOTTING; LIVER NEOPLASMS; NAPHTHOLS; REAL-TIME POLYMERASE CHAIN REACTION; REPRESSOR PROTEINS; RNA, MESSENGER; SENSITIVITY AND SPECIFICITY; TRANSFECTION; TUMOR CELLS, CULTURED; VIRAL CORE PROTEINS;

EID: 84864284903     PISSN: 01632116     EISSN: 15732568     Source Type: Journal    
DOI: 10.1007/s10620-012-2160-1     Document Type: Article

References (35)

1. Tellinghuisen TL, Rice CM. Interaction between hepatitis C virus proteins and host cell factors. Curr Opin Microbiol. 2002;5: 419-427.
2. Saito I, Miyamura T, Ohbayashi A, et al. Hepatitis C virus infection is associated with the development of hepatocellular carcinoma. Proc Natl Acad Sci USA. 1990;87:6547-6549.
3. Simonetti RG, Cammà C, Fiorello F, et al. Hepatitis C virus infection as a risk factor for hepatocellular carcinoma in patients with cirrhosis. A case-control study. Ann Intern Med. 1992;116: 97-102.
4. Okamoto H, Kurai K, Okada SI, et al. Full length sequence of a hepatitis C virus genome having poor homology to reported isolates - comparative study of four distinct genotypes. Virology. 1992;188:331-341.
5. Ripoli M, Pazienza V. Impact of hepatitis C virus genetic differences on pathobiology of disease. Expert Rev Anti Infect Ther. 2011;9:747-759.
6. Moriya K, Fujie H, Shintani Y, et al. The core protein of hepatitis C virus induces hepatocellular carcinoma in transgenic mice. Nat Med. 1998;4:1065-1717.
7. Yoshida T, Hanada T, Tokuhisa T, et al. Activation of STAT3 by the hepatitis C virus core protein leads to cellular transformation. J Exp Med. 2002;196:641-653. (Pubitemid 35024442)
8. Pazienza V, Clement S, Pugnale P, et al. The hepatitis C virus core protein of genotypes 3a and 1b downregulates insulin receptor substrate 1 through genotype-specific mechanisms. Hepatology. 2007;45:1164-1171. (Pubitemid 46775778)
9. Pazienza V, Clement S, Pugnale P, et al. Gene expression profile of Huh-7 cells expressing hepatitis C virus genotype1b or 3a core proteins. Liver Int. 2009;29:661-669.
10. Bruno S, Crosignani A, Maisonneuve P, Rossi S, Silini E, Mondelli MU. Hepatitis C virus genotype 1b as a major risk factor associated with hepatocellular carcinoma in patients with cirrhosis: a seventeen-year prospective cohort study. Hepatology. 2007;46:1350-1356. (Pubitemid 350155516)
11. Raimondi S, Bruno S, Mondelli MU, Maisonneuve P. Hepatitis C virus genotype 1b as a risk factor for hepatocellular carcinoma development: a meta-analysis. J Hepatol. 2009;50:1142-1154.
12. Levrero M. Viral hepatitis and liver cancer: the case of hepatitis C. Oncogene. 2006;25:3834-3847. (Pubitemid 43980479)
13. Ripoli M, Barbano R, Balsamo T, Piccoli C, Brunetti V, Coco M, Mazzoccoli G, Vinciguerra M, Pazienza V. Hypermethylated levels of E-cadherin promoter in Huh-7 cells expressing the HCV core protein. Virus Res. 2011;160:74-81.
14. Takeichi M. Cadherins in cancer: implications for invasion and metastasis. Curr Opin Cell Biol. 1993;5:806-811.
15. Abid K, Pazienza V, de Gottardi A, et al. An in vitro model of hepatitis C virus genotype 3a-associated triglycerides accumulation. J Hepatol. 2005;42:744-751.
16. Brooks CL, Gu W. How does SIRT1 affect metabolism, senescence and cancer? Nat Rev Cancer. 2009;9:123-128.
17. Arora P, Kim EO, Jung JK, Jang KL. Hepatitis C virus core protein downregulates E-cadherin expression via activation of DNA methyltransferase 1 and 3b. Cancer Lett. 2008;261:244-252.
18. Csepregi A, Röcken C, Hoffmann J, et al. APC promoter methylation and protein expression in hepatocellular carcinoma. J Cancer Res Clin Oncol. 2008;134:579-589.
19. Dejeux E, Audard V, Cavard C, Gut IG, Terris B, Tost J. Rapid identification of promoter hypermethylation in hepatocellular carcinoma by pyrosequencing of etiologically homogeneous sample pools. J Mol Diagn. 2007;9:510-520. (Pubitemid 47434118)
20. Zhang Q, Wang HY, Woetmann A, Raghunath PN, Odum N, Wasik MA. STAT3 induces transcription of the DNA methyltransferase 1 gene (DNMT1) in malignant T lymphocytes. Blood. 2006;108:1058-1064. (Pubitemid 44154645)
21. Chen J, Zhang B, Wong N, Lo AW, To KF, Chan AW, Ng MH, Ho CY, Cheng SH, Lai PB, Yu J, Ng HK, Ling MT, Huang AL, Cai XF, Ko BC. Sirtuin 1 is upregulated in a subset of hepato-cellular carcinomas where it is essential for telomere maintenance and tumor cell growth. Cancer Res. 2011;71:4138-149.
22. Choi HN, Bae JS, Jamiyandorj U, et al. Expression and role of SIRT1 in hepatocellular carcinoma. Oncol Rep. 2011;26:503-510. doi:10.3892/or.2011.1301.
23. Hp Li, Yw Leu, Ys Chang. Epigenetic changes in virus-associated human cancers. Cell Res. 2005;15:262-271. (Pubitemid 41642882)
24. Laurson J, Khan S, Chung R, Cross K, Raj K. Epigenetic repression of E-cadherin by human papillomavirus 16 E7 protein. Carcinogenesis. 2010;31:918-926.
25. Vivekanandan P, Daniel HD, Kannangai R, Martinez-Murillo F, Torbenson M. Hepatitis B virus replication induces methylation of both host and viral DNA. J Virol. 2010;84:4321-4329.
26. Waris G, Turkson J, Hassanein T, Siddiqui A. Hepatitis C virus (HCV) constitutively activates STAT-3 via oxidative stress: role of STAT-3 in HCV replication. J Virol. 2005;79:1569-1580. (Pubitemid 40459104)
27. Tacke RS, Tosello-Trampont A, Nguyen V, Mullins DW, Hahn YS. Extracellular hepatitis C virus core protein activates STAT3 in human monocytes/macrophages/dendritic cells via an IL-6 autocrine pathway. J Biol Chem. 2011;286:10847-10855.
28. Yu H, Jove R. The stats of cancer - new molecular targets come of age. Nat Rev Cancer. 2004;4:97-1058.
29. Turkson J, Kim JS, Zhang S, et al. Novel peptidomimetic inhibitors of signal transducer and activator of transcription 3 dimerization and biological activity. Mol Cancer Ther. 2004;3: 261-269. (Pubitemid 39193701)
30. Ulane CM, Rodriguez JJ, Parisien JP, Horvath M. STAT3 ubiq-uitylation and degradation by mumps virus suppress cytokine and oncogene signaling. J Virol. 2003;77:6385-6393. (Pubitemid 36588409)
31. Zhang Q, Wang HY, Marzec M, Raghunath PN, Nagasawa T, Wasik MA. STAT3- and DNA methyltransferase 1-mediated epigenetic silencing of SHP-1 tyrosine phosphatase tumor suppressor gene in malignant T lymphocytes. Proc Natl Acad Sci USA. 2005;102:6948-6953. (Pubitemid 40675422)
32. Paredes R, Esteller M. Cancer epigenetics reaches mainstream oncology. Nature Med. 2011;17:330-339.
33. O'Hagan HM, Mohammad HP, Baylin SB. Double strand breaks can initiate gene silencing and SIRT1-dependent onset of DNA methylation in an exogenous promoter CpG island. PLoS Genet. 2008;4:e1000155.
34. Nie Y, Erion DM, Yuan Z, Dietrich M, Shulman GI, Horvath TL, Gao Q. STAT3 inhibition of gluconeogenesis is downregulated by SirT1. Nat Cell Biol. 2009;11:492-500.
35. Bernier M, Paul RK, Martin-Montalvo A, Scheibye-Knudsen M, Song S, He HJ, Armour SM, Hubbard BP, Bohr VA, Wang L, Zong Y, Sinclair DA, de Cabo R. Negative regulation of STAT3 protein-mediated cellular respiration by SIRT1 protein. J Biol Chem. 2011;286:19270-19279.