Characterization of PEGylated biopharmaceutical products by LC/MS and LC/MS/MS

Methods in Molecular Biology

Volumn 899, Issue , 2012, Pages 351-363

  Huang, Lihua ^a   Gough, P Clayton ^a  

^a ELI LILLY AND COMPANY   (United States)

Author keywords

CID; Diethylmethylamine; In source fragmentation; PEGylation; PEGylation site mapping; Post column addition; Triethylamine

Indexed keywords

MACROGOL DERIVATIVE; PEPTIDE; PROTEIN;

ARTICLE; CHEMISTRY; HUMAN; LIQUID CHROMATOGRAPHY; METHODOLOGY; TANDEM MASS SPECTROMETRY;

CHROMATOGRAPHY, LIQUID; HUMANS; PEPTIDES; POLYETHYLENE GLYCOLS; PROTEINS; TANDEM MASS SPECTROMETRY;

EID: 84864140639     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-921-1_22     Document Type: Article

References (13)

1. Walsh G (2000) Biopharmaceutical benchmarks. Nat Biotechnol 18:831-833
2. Bruckdorfer T, Marder O, Albericio F (2004) From production of peptides in milligram amounts for research to multi-tons quantities for drugs of the future. Curr Pharm Biotechnol 5:29-43
3. Abuchowski A, McCoy JR, Palczuk NC, van Es T, Davis FF (1977) Effect of covalent attachment of polyethylene glycol on immunogenicity and circulating life of bovine liver catalase. J Biol Chem 252:3582-3586
4. Roberts MJ, Bentley MD, Harris JM (2002) Chemistry for peptide and protein PEGylation. Adv Drug Deliv Rev 54:459-476
5. Stigsnaes P, Frokjaer S, Bjerregaard S, van de Weert M, Peter Kingshott P, Moeller EH (2007) Characterisation and physical stability of PEGylated glucagon. Int J Pharm 330:89-98
6. Cunningham-Rundles C, Zhou Z, Grif fith B, Keenan J (1992) Biological activities of polyethylene-glycol immunoglobulin conjugates resistance to enzymatic degradation. J Immunol Methods 152:177-190
7. Chapman AP (2002) PEGylated antibodies and antibody fragments for improved therapy: A review. Adv Drug Deliv Rev 54:531-545
8. Veronese FM, Mero A (2008) The impact of PEGylation on biological therapies. BioDrugs 22:315-329
9. Huang L, Gough PG, DeFelippis MR (2009) Characterization of poly(ethylene glycol) and PEGylated products by LC/MS with postcolumn addition of amines. Anal Chem 81: 567-577
10. Lu X, Gough PG, DeFelippis MR, Huang L (2010) Elucidation of PEGylation site with a combined approach of in-source fragmentation and CID MS/MS. J Am Soc Mass Spectrom 21:810-818
11. Robinson EW, Garcia DE, Leib RD, Williams ER (2006) Enhanced mixture analysis of poly(ethylene glycol) using high- field asymmetric waveform ion mobility spectrometry combined with Fourier transform ion cyclotron resonance mass spectrometry. Anal Chem 78: 2190-2198
12. Hanton SD (2001) Mass spectrometry of polymers and polymer surfaces. Chem Rev 101:527-569
13. Cooper AR (1989) Determination of molecular weight. In: Winefordner JD (ed) Chemical analysis, vol 103. Wiley, New York, p 526