Development of a multiplex PCR assay for rapid identification of Burkholderia pseudomallei, Burkholderia thailandensis, Burkholderia mallei and Burkholderia cepacia complex

Journal of Microbiological Methods

Volumn 90, Issue 3, 2012, Pages 305-308

  Koh, Seng Fook ^a   Tay, Sun Tee ^a   Sermswan, Rasana ^b,c   Wongratanacheewin, Surasakdi ^b,c   Chua, Kek Heng ^a   Puthucheary, Savithri D ^a  

^a UNIVERSITY OF MALAYA   (Malaysia)

^b KHON KAEN UNIVERSITY   (Thailand)

^c KHON KAEN UNIVERSITY   (Thailand)

Author keywords

Burkholderia mallei and Burkholderia cepacia complex; Burkholderia pseudomallei; Burkholderia thailandensis; Multiplex PCR assay

Indexed keywords

RNA 16S; TRANSACTIVATOR PROTEIN;

ARTICLE; BACTERIAL GENE; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; BURKHOLDERIA; BURKHOLDERIA CEPACIA COMPLEX; BURKHOLDERIA MALLEI; BURKHOLDERIA PSEUDOMALLEI; BURKHOLDERIA THAILANDENSIS; CLINICAL LABORATORY; DIAGNOSTIC VALUE; DNA FLANKING REGION; EPIDEMIC; GENE AMPLIFICATION; GENE LOCUS; GENE TARGETING; GENETIC MARKER; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROCESS DEVELOPMENT; PROTEIN DOMAIN; SEQUENCE ALIGNMENT; SPECIES DIFFERENTIATION; VALIDATION PROCESS;

BURKHOLDERIA CEPACIA COMPLEX; BURKHOLDERIA MALLEI; BURKHOLDERIA PSEUDOMALLEI; ELECTROPHORESIS, AGAR GEL; GENES, BACTERIAL; MOLECULAR TYPING; MULTIPLEX POLYMERASE CHAIN REACTION; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S;

BURKHOLDERIA CEPACIA COMPLEX; BURKHOLDERIA MALLEI; BURKHOLDERIA PSEUDOMALLEI; BURKHOLDERIA THAILANDENSIS;

EID: 84864112526     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2012.06.002     Document Type: Article

References (12)

1. Bikandi J., San Millan R., Rementeria A., Garaizar J. In silico analysis of complete bacterial genomes: PCR, AFLP-PCR and endonuclease restriction. Bioinformatics 2004, 20:798-799.
2. Chantratita N., Meumann E., Thanwisai A., Limmathurotsakul D., Wuthiekanun V., Wannapasni S., et al. Loop-mediated isothermal amplification method targeting the TTS1 gene cluster for detection of Burkholderia pseudomallei and diagnosis of melioidosis. J. Clin. Microbiol. 2008, 46:568-573.
3. Cheng A.C., Currie B.J. Melioidosis: epidemiology, pathophysiology, and management. Clin. Microbiol. Rev. 2005, 18:383-416.
4. Glass M.B., Gee J.E., Steigerwalt A.G., Cavuoti D., Barton T., Hardy R.D., Godoy D., Spratt B.G., Clark T.A., Wilkins P.P. Pneumonia and septicemia caused by Burkholderia thailandensis in the United States. J. Clin. Microbiol. 2006, 44:4601-4604.
5. Ho C.C., Lau C.C., Martelli P., Chan S.Y., Tse C.W., Wu A.K., Yuen K.Y., Lau S.K., Woo P.C. Novel pan-genomic analysis approach in target selection for multiplex PCR identification and detection of Burkholderia pseudomallei, Burkholderia thailandensis, and Burkholderia cepacia complex species: a proof-of-concept study. J. Clin. Microbiol. 2011, 49:814-821.
6. Kim H.S., Schell M., Yu Y., Ulrich R., Sarria S., Nierman W., et al. Bacterial genome adaptation to niches: divergence of the potential virulence genes in three Burkholderia species of different survival strategies. BMC Genomics 2005, 6:174.
7. Lee M.A., Wang D., Yap E.H. Detection and differentiation of Burkholderia pseudomallei, Burkholderia mallei and Burkholderia thailandensis by multiplex PCR. FEMS Immunol. Med. Microbiol. 2005, 43:413-417.
8. Pavel A.B., Vasile C.I. PyElph-a software tool for gel images analysis and phylogenetics. BMC Bioinform. 2012, 13:9.
9. Puthucheary S.D., Parasakthi N., Lee M.K. Septicaemic melioidosis: a report of 50 cases from Malaysia. Trans. R. Soc. Trop. Med. Hyg. 1992, 86:683-685.
10. Rozen S., Skaletsky H. Primer3 on the WWW for general users and for biologist programmers. Methods Mol. Biol. 2000, 132:365-386.
11. Tanpiboonsak S., Paemanee A., Bunyarataphan S., Tungpradabkul S. PCR-RFLP based differentiation of Burkholderia mallei and Burkholderia pseudomallei. Mol. Cell. Probes 2004, 18:97-101.
12. Wongtrakoongate P., Mongkoldhumrongkul N., Chaijan S., Kamchonwongpaisan S., Tungpradabkul S. Comparative proteomic profiles and the potential markers between Burkholderia pseudomallei and Burkholderia thailandensis. Mol. Cell. Probes 2007, 21:81-91.