Establishment of loop-mediated isothermal amplification (LAMP) for rapid detection of Brucella spp. and application to milk and blood samples

Journal of Microbiological Methods

Volumn 90, Issue 3, 2012, Pages 292-297

  Song, Liuyan ^a   Li, Juntao ^a   Hou, Shuiping ^a   Li, Xunde ^b   Chen, Shouyi ^a,b  

^a GUANGZHOU CENTER FOR DISEASE CONTROL AND PREVENTION   (China)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Blood; Brucella spp.; Loop mediated isothermal amplification (LAMP); Milk; Rapid detection; Strand displacement reaction

Indexed keywords

DEOXYRIBONUCLEOTIDE; MAGNESIUM; PRIMER DNA;

ARTICLE; BACTERIAL GENE; BACTERIUM DETECTION; BLOOD ANALYSIS; BLOOD SAMPLING; BRUCELLA; BRUCELLA ABORTUS; BRUCELLA MELITENSIS; BRUCELLA OVIS; BRUCELLA SUIS; BRUCELLOSIS; COLONY FORMING UNIT; CONCENTRATION RESPONSE; CONTROLLED STUDY; FOOD ANALYSIS; GENE IDENTIFICATION; LIMIT OF DETECTION; LOOP MEDIATED ISOTHERMAL AMPLIFICATION; MILK; NONHUMAN; NUCLEOTIDE SEQUENCE; OMP25 GENE; PRIORITY JOURNAL; PROCESS DEVELOPMENT; PROCESS OPTIMIZATION; REACTION TIME; SENSITIVITY AND SPECIFICITY; SHEEP; TEMPERATURE MEASUREMENT;

ANIMALS; BRUCELLA; BRUCELLOSIS; CATTLE; CATTLE DISEASES; DEOXYRIBONUCLEOTIDES; DNA-DIRECTED DNA POLYMERASE; GENES, BACTERIAL; LIMIT OF DETECTION; MAGNESIUM; MILK; NUCLEIC ACID AMPLIFICATION TECHNIQUES; SHEEP; TEMPERATURE;

ANIMALIA; BRUCELLA; OVIS ARIES;

EID: 84864095446     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2012.05.024     Document Type: Article

References (18)

1. Dahouk A.S., Nöckler K., Hensel A., Tomaso H., Scholz H.C., Hagen R.M., Neubauer H. Human brucellosis in a nonendemic country: a report from Germany, 2002 and 2003. Eur. J. Clin. Microbiol. Infect. Dis. 2002, 24(27):450-456.
2. Enosawa M., Kageyama S., Sawai K., Watanabe K., Notomi T., Onoe S., Mori Y., Yokomizo Y. Use of loop-mediated isothermal amplification of the IS900 sequence for rapid detection of cultured Mycobacterium avium subsp. paratuberculosis. J. Clin. Microbiol. 2003, 41(9):4359-4365.
3. Ettinger S.J., Feldman E.C. Textbook of Veterinary Internal Medicine 1995, W.B. Saunders Company. 4th ed.
4. Haerry T.E., Gehring W.J. Intron of the mouse Hoxa-7 gene contains conserved homeodomain binding sites that can function as an enhancer element in Drosophilas. Proc. Natl. Acad. Sci. U. S. A. 1996, 93(24):13884-13889.
5. Ikadai H., Tanaka H., Shibahara N., Matsuu A., Uechi M., Itoh N., Oshiro S., Kudo N., Igarashi I., Oyamada T. Molecular evidence of infections with Babesia gibsoni parasites in Japan and evaluation of the diagnostic potential of a loop-mediated isothermal amplification method. J. Clin. Microbiol. 2004, 42(6):2465-2469.
6. Lu X., Mo Z.Y., Zhao H.B., Yan H., Shi L. LAMP-based method for a rapid identification of Legionella spp. and Legionella pneumophila. Appl. Microbiol. Biotechnol. 2011, 92(1):179-187.
7. Maruyama F., Kenzaka T., Yamaguchi N., Tani K., Nasu M. Detection of bacteria carrying the stx2 gene by in situ loop-mediated isothermal amplification. Appl. Environ. Microbiol. 2003, 69(8):5023-5028.
8. Matyas Z., Fujikura T. Brucellosis as a world problem. Dev. Biol. Stand. 1984, 56:3-20.
9. Notomi T., Okayama H., Masubuchi H., Yonekawa T., Watanabe K., Amino N., Hase T. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000, 28(12):E63.
10. Ping Z., Yang G.L., Chen D.J. Progress in studies on brucellosis in cattle. China Cattle Sci. 2008, 34(5):50-52.
11. Qiu S.D. Progress in studies on brucellosis. Chin. J. Control Endem. Dis. 2004, 19(4):204-212.
12. Radostits O.M., Gay C.C., Blood D.C., Hinchcliff K.W. Veterinary medicine, a textbook of the diseases of cattle, sheep, pigs, goats and horses 2000, Harcourt Publishers Ltd., London.
13. Shao Y., Zhu S., Jin C. Development of multiplex loop-mediated isothermal amplification-RFLP (mLAMP-RFLP) to detect Salmonella spp. and Shigella spp. in milk. Int. J. Food Microbiol. 2011, 148(2):75-79.
14. Sreevatsan S., Bookout J.B., Ringpis F., Perumaalla V.S., Ficht T.A., Adams L.G., Hagius S.D., Elzer P.H., Bricker B.J., Kumar G.K., Rajasekhar M., Isloor S., Barathur R.R. A multiplex approach to molecular detection of Brucella abortus and/or Mycobacterium bovis infection in cattle. J. Clin. Microbiol. 2000, 38(7):2602-2610.
15. Tang L.J., Chen C.F., Wang Y.Z. A study on rapid diagnosis of brucellosis in livestock products using nested PCR. Shanghai J. Anim. Husb. Vet. Med. 2008, 4:39-41.
16. Wang J., Xu W.M. Progress in studies on the serum diagnosis of brucellosis. Chin. J. Pathog. Biol. 2008, 3(2):149-152.
17. Wilkinson L. Brucellosis. The Cambridge World History of Human Disease 1993, Cambridge University Press. K.F. Kiple (Ed.).
18. Yoshikawa T., Ihira M., Akimoto S., Usui C., Miyake F., Suga S., Enomoto Y., Suzuki R., Nishiyama Y., Asano Y. Detection of human herpesvirus 7 DNA by loop-mediated isothermal amplification. J. Clin. Microbiol. 2004, 42(3):1348-1352.