Evidence for an oxygen-sensitive iron-sulfur cluster in an immature large subunit species of Escherichia coli [NiFe]-hydrogenase 2

Biochemical and Biophysical Research Communications

Volumn 424, Issue 1, 2012, Pages 158-163

  Soboh, Basem ^a   Kuhns, Martin ^a,c   Braussemann, Mario ^a   Waclawek, Mandy ^a   Muhr, Enrico ^a   Pierik, Antonio J ^b   Sawers, R Gary ^a  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

^b PHILIPPS UNIVERSITY MARBURG   (Germany)

^c GOETHE UNIVERSITY   (Germany)

Author keywords

NiFe hydrogenase; Iron sulfur cluster; Large subunit; Maturation; Processing

Indexed keywords

HYDROGENASE; IRON SULFUR PROTEIN; PENTAPEPTIDE; POLYPEPTIDE;

ARTICLE; BINDING SITE; CONFORMATIONAL TRANSITION; ESCHERICHIA COLI; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN ISOLATION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; STOICHIOMETRY; SYNTHESIS;

CARBOXYL AND CARBAMOYL TRANSFERASES; CARRIER PROTEINS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HYDROGENASE; IRON-SULFUR PROTEINS; MUTATION; OLIGOPEPTIDES; OXIDOREDUCTASES; OXYGEN;

ESCHERICHIA COLI;

EID: 84864093074     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2012.06.096     Document Type: Article

References (30)

1. Vignais P.M., Billoud B., Meyer J. Classification and phylogeny of hydrogenases. FEMS Microbiol. Rev. 2001, 25:455-501.
2. Volbeda A., Charon M.H., Piras C., Hatchikian E.C., Frey M., Fontecilla-Camps J.C. Crystal structure of the nickel--iron hydrogenase from Desulfovibrio gigas. Nature 1995, 373:580-587.
3. Forzi L., Sawers R.G. Maturation of [NiFe]-hydrogenases in Escherichia coli. Biometals 2007, 20:565-578.
4. Böck A., King P., Blokesch M., Posewitz M. Maturation of hydrogenases. Adv. Microb. Physiol. 2006, 51:1-71.
5. Magalon A., Blokesch M., Zehelein E., Böck A. Fidelity of metal insertion into hydrogenases. FEBS Lett. 2001, 499:73-76.
6. Leach M.R., Zamble D.B. Metallocenter assembly of the hydrogenase enzymes. Curr. Opin. Chem. Biol. 2007, 11:159-165.
7. Rossmann R., Sauter M., Lottspeich F., Böck A. Maturation of the large subunit (HYCE) of Escherichia coli hydrogenase 3 requires nickel incorporation followed by C-terminal processing at Arg537. Eur. J. Biochem. 1994, 220:377-384.
8. Magalon A., Böck A. Dissection of the maturation reactions of the [NiFe]-hydrogenase 3 from Escherichia coli taking place after nickel incorporation. FEBS Lett. 2000, 473:254-258.
9. Casadaban M.J. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. J. Mol. Biol. 1976, 104:541-555.
10. Redwood M., Mikheenko I., Sargent F., Macaskie L. Dissecting the roles of Escherichia coli hydrogenases in biohydrogen production. FEMS Microbiol. Lett. 2007, 278:48-55.
11. Paschos A., Bauer A., Zimmermann A., Zehelein E., Böck A. HypF, a carbamoyl phosphate-converting enzyme involved in [NiFe]-hydrogenase maturation. J. Biol. Chem. 2002, 277:49945-49951.
12. Pinske C., Sawers R.G. The A-type carrier protein ErpA is essential for formation of an active formate-nitrate respiratory pathway in Escherichia coli K-12. J. Bacteriol. 2012, 194:346-353.
13. Pinske C., Krüger S., Soboh B., Ihling C., Kuhns M., Braussemann M., Jaroschinsky M., Sauer C., Sargent F., Sinz A., Sawers R.G. Efficient electron transfer from hydrogen to benzyl viologen by the [NiFe]-hydrogenases of Escherichia coli is dependent on the coexpression of the Iron-sulfur cluster-containing small subunit. Arch. Microbiol. 2011, 193:893-903.
14. Kitagawa M., Ara T., Arifuzzaman M., Ioka-Nakamichi T., Inamoto E., Toyonaga H., Mori H. Complete set of ORF clones of Escherichia coli ASKA library (A complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 2005, 12:291-299.
15. Begg Y., Whyte J., Haddock B. The identification of mutants of Escherichia coli deficient in formate dehydrogenase and nitrate reductase activities using dye indicator plates. FEMS Microbiol. Lett. 1977, 2:47-50.
16. Soboh B., Pinske C., Kuhns M., Waclawek M., Ihling C., Trchounian K., Trchounian A., Sinz A., Sawers R.G. The respiratory molybdo-selenoprotein formate dehydrogenases of Escherichia coli have hydrogen: benzyl viologen oxidoreductase activity. BMC Microbiol. 2011, 11:173.
17. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685.
18. Lowry O., Rosebrough N., Farr A., Randall R. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 1951, 193:265-275.
19. Netz D.J.A., Stith C.M., Stümpfig M., Köpf G., Vogel D., Genau H.M., Stodola J.L., Lill R., Burgers P.M.J., Pierik A.J. Eukaryotic DNA polymerases require an Iron-sulfur cluster for the formation of active complexes. Nat. Chem. Biol. 2012, 8:125-132.
20. Fish W.W. Rapid colorimetric micromethod for the quantitation of complexed iron in biological samples. Meth. Enzymol. 1988, 158:357-364.
21. Cline J.D. Spectrophotometric determination of hydrogen sulfide in natural waters. Limnol. Oceanogr. 1969, 14:454-458.
22. Paul M., McSheehy S. Elementspezies bestimmen mit ICP-MS. Nachr. Chem. 2007, 55:766-769.
23. Menon N.K., Chatelus C.Y., Dervartanian M., Wendt J.C., Shanmugam K.T., Peck H.D., Przybyla A.E. Cloning, sequencing, and mutational analysis of the hyb operon encoding Escherichia coli hydrogenase 2. J. Bacteriol. 1994, 176:4416-4423.
24. Pinske C., Sawers R.G. Delivery of Iron-sulfur clusters to the hydrogen-oxidizing [NiFe]-hydrogenase in Escherichia coli requires the A-type carrier proteins ErpA and IscA. PLoS One 2012, 7:e31755.
25. Py B., Moreau P.L., Barras F. Fe-S cluster, fragile sentinels of the cell. Curr. Opin. Microbiol. 2011, 14:218-223.
26. Theodoratou E., Paschos A., Mintz-Weber S., Böck A. Analysis of the cleavage specificity of the endopeptidase involved in the maturation of the large subunit of hydroenase 3 from Escherichia coli. Arch. Microbiol. 2000, 173:110-116.
27. Zhang M., Pradel N., Mandrand-Berthelot M.A., Yu Z., Wu L.F. Effect of alteration of the C-terminal extension on the maturation and folding of the large subunit of the Escherichia coli hydrogenase-2. Biochemie 2003, 85:575-579.
28. 2 sensitivity of the transcription factor FNR is controlled by Ser24 modulating the kinetics of [4Fe-4S] to [2Fe-2S] conversion. Proc. Natl. Acad. Sci. USA 2009, 106:4659-4664.
29. 2 on the Fe-S cluster biogenesis requirements of Escherichia coli FNR. J. Biol. Chem. 2008, 384:798-811.
30. Sasaki D., Watanabe S., Kanai T., Atomi H., Imanaka T., Miki K. Characterization and in vitro interaction study of a [NiFe] hydrogenase large subunit from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1. Biochem. Biophys. Res. Commun. 2012, 417:192-196.